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Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows

BACKGROUND: As a newly characterized type of noncoding RNA, circular RNA (circRNA) has been shown to have functions in diverse biological processes of animals. It has been reported that several noncoding RNAs may regulate animals’ response to heat stress which can be easily induced by hyperthermia i...

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Autores principales: Zhang, Haojie, Hu, Baoyu, Xiong, Jiali, Chen, Ting, Xi, Qianyun, Luo, Junyi, Jiang, Qingyan, Sun, Jiajie, Zhang, Yongliang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929353/
https://www.ncbi.nlm.nih.gov/pubmed/31870281
http://dx.doi.org/10.1186/s12864-019-6377-7
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author Zhang, Haojie
Hu, Baoyu
Xiong, Jiali
Chen, Ting
Xi, Qianyun
Luo, Junyi
Jiang, Qingyan
Sun, Jiajie
Zhang, Yongliang
author_facet Zhang, Haojie
Hu, Baoyu
Xiong, Jiali
Chen, Ting
Xi, Qianyun
Luo, Junyi
Jiang, Qingyan
Sun, Jiajie
Zhang, Yongliang
author_sort Zhang, Haojie
collection PubMed
description BACKGROUND: As a newly characterized type of noncoding RNA, circular RNA (circRNA) has been shown to have functions in diverse biological processes of animals. It has been reported that several noncoding RNAs may regulate animals’ response to heat stress which can be easily induced by hyperthermia in summer. However, the expression and functions of circRNAs in the pituitary of sows and whether they participate in heat stress adaption are still unclear. RESULTS: In this study, we found that high temperature over the thermoneutral zone of sows during the summer increased the serum heat shock protein 70 (HSP70) level, decreased the superoxide dismutase (SOD) vitality and prolactin (PRL) concentration, and induced heat stress in sows. Then, we explored circRNA in the pituitary of heat-stressed and normal sows using RNA sequencing and bioinformatics analysis. In total, 12,035 circRNAs were detected, with 59 circRNAs differentially expressed, including 42 up-regulated and 17 down-regulated circRNAs in pituitaries of the heat-stressed sows. Six randomly selected circRNAs were identified through reverse transcription PCR followed by DNA sequencing and other 7 randomly selected differentially expressed circRNAs were verified by quantitative real-time PCR analysis. The predicted target genes regulated by circRNAs through sponging microRNAs (miRNAs) were enriched in metabolic pathway. Furthermore, the predicted circRNA–miRNA–mRNA interactions showed that some circRNAs might sponge miRNAs to regulate pituitary-specific genes and heat shock protein family members, indicating circRNA’s roles in pituitary hormone secretion and heat stress response. CONCLUSIONS: Our results provided a meaningful reference to understand the functions of circRNA in the porcine pituitary and the mechanisms by which circRNA may participate in animals’ response to heat stress.
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spelling pubmed-69293532019-12-30 Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows Zhang, Haojie Hu, Baoyu Xiong, Jiali Chen, Ting Xi, Qianyun Luo, Junyi Jiang, Qingyan Sun, Jiajie Zhang, Yongliang BMC Genomics Research Article BACKGROUND: As a newly characterized type of noncoding RNA, circular RNA (circRNA) has been shown to have functions in diverse biological processes of animals. It has been reported that several noncoding RNAs may regulate animals’ response to heat stress which can be easily induced by hyperthermia in summer. However, the expression and functions of circRNAs in the pituitary of sows and whether they participate in heat stress adaption are still unclear. RESULTS: In this study, we found that high temperature over the thermoneutral zone of sows during the summer increased the serum heat shock protein 70 (HSP70) level, decreased the superoxide dismutase (SOD) vitality and prolactin (PRL) concentration, and induced heat stress in sows. Then, we explored circRNA in the pituitary of heat-stressed and normal sows using RNA sequencing and bioinformatics analysis. In total, 12,035 circRNAs were detected, with 59 circRNAs differentially expressed, including 42 up-regulated and 17 down-regulated circRNAs in pituitaries of the heat-stressed sows. Six randomly selected circRNAs were identified through reverse transcription PCR followed by DNA sequencing and other 7 randomly selected differentially expressed circRNAs were verified by quantitative real-time PCR analysis. The predicted target genes regulated by circRNAs through sponging microRNAs (miRNAs) were enriched in metabolic pathway. Furthermore, the predicted circRNA–miRNA–mRNA interactions showed that some circRNAs might sponge miRNAs to regulate pituitary-specific genes and heat shock protein family members, indicating circRNA’s roles in pituitary hormone secretion and heat stress response. CONCLUSIONS: Our results provided a meaningful reference to understand the functions of circRNA in the porcine pituitary and the mechanisms by which circRNA may participate in animals’ response to heat stress. BioMed Central 2019-12-23 /pmc/articles/PMC6929353/ /pubmed/31870281 http://dx.doi.org/10.1186/s12864-019-6377-7 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Haojie
Hu, Baoyu
Xiong, Jiali
Chen, Ting
Xi, Qianyun
Luo, Junyi
Jiang, Qingyan
Sun, Jiajie
Zhang, Yongliang
Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows
title Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows
title_full Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows
title_fullStr Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows
title_full_unstemmed Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows
title_short Genomewide analysis of circular RNA in pituitaries of normal and heat-stressed sows
title_sort genomewide analysis of circular rna in pituitaries of normal and heat-stressed sows
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929353/
https://www.ncbi.nlm.nih.gov/pubmed/31870281
http://dx.doi.org/10.1186/s12864-019-6377-7
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