Long Noncoding RNA (lncRNA) FOXD2-AS1 Promotes Cell Proliferation and Metastasis in Hepatocellular Carcinoma by Regulating MiR-185/AKT Axis

BACKGROUND: The aim of this study was to investigate the effects and mechanisms of long noncoding (lnc) RNA FOXD2-AS1 in hepatocellular carcinoma development. MATERIAL/METHODS: Collecting the 3 pairs of adjacent and hepatocellular carcinoma tissue and analysis by gene chip. Evaluating the FOXD2-AS1...

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Autores principales: Chen, Zheng, Zhang, Zhen, Zhao, Dongbo, Feng, Wei, Meng, Fanlai, Han, Shihui, Lin, Bin, Shi, Xin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scientific Literature, Inc. 2019
Materias:
Lab/In Vitro Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929557/
https://www.ncbi.nlm.nih.gov/pubmed/31841454
http://dx.doi.org/10.12659/MSM.918230
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author Chen, Zheng
Zhang, Zhen
Zhao, Dongbo
Feng, Wei
Meng, Fanlai
Han, Shihui
Lin, Bin
Shi, Xin
author_facet Chen, Zheng
Zhang, Zhen
Zhao, Dongbo
Feng, Wei
Meng, Fanlai
Han, Shihui
Lin, Bin
Shi, Xin
author_sort Chen, Zheng
collection PubMed
description BACKGROUND: The aim of this study was to investigate the effects and mechanisms of long noncoding (lnc) RNA FOXD2-AS1 in hepatocellular carcinoma development. MATERIAL/METHODS: Collecting the 3 pairs of adjacent and hepatocellular carcinoma tissue and analysis by gene chip. Evaluating the FOXD2-AS1 expression by in situ hybridization assay. Evaluating the FOXD2-AS1 to Bel-7402 biological activity in vitro study by Cell Counting Kit-8, flow cytometry, Transwell and wound healing assay and correlation between miR-185 by dual-luciferase reporter assay. The relative proteins expressions were evaluated by western blot assay. RESULTS: FOXD2-AS1 was significantly upregulation in hepatocellular carcinoma tissues. FOXD2-AS1 knockdown suppressed Bel-7401 cell biological activities (proliferation, invasion, and migration) with miR-185 overexpression and AKT depressing in cell expression. CONCLUSIONS: LncRNA FOXD2-AS1 promoted hepatocellular carcinoma development by regulation miR-185/AKT axis.
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spelling pubmed-69295572019-12-26 Long Noncoding RNA (lncRNA) FOXD2-AS1 Promotes Cell Proliferation and Metastasis in Hepatocellular Carcinoma by Regulating MiR-185/AKT Axis Chen, Zheng Zhang, Zhen Zhao, Dongbo Feng, Wei Meng, Fanlai Han, Shihui Lin, Bin Shi, Xin Med Sci Monit Lab/In Vitro Research BACKGROUND: The aim of this study was to investigate the effects and mechanisms of long noncoding (lnc) RNA FOXD2-AS1 in hepatocellular carcinoma development. MATERIAL/METHODS: Collecting the 3 pairs of adjacent and hepatocellular carcinoma tissue and analysis by gene chip. Evaluating the FOXD2-AS1 expression by in situ hybridization assay. Evaluating the FOXD2-AS1 to Bel-7402 biological activity in vitro study by Cell Counting Kit-8, flow cytometry, Transwell and wound healing assay and correlation between miR-185 by dual-luciferase reporter assay. The relative proteins expressions were evaluated by western blot assay. RESULTS: FOXD2-AS1 was significantly upregulation in hepatocellular carcinoma tissues. FOXD2-AS1 knockdown suppressed Bel-7401 cell biological activities (proliferation, invasion, and migration) with miR-185 overexpression and AKT depressing in cell expression. CONCLUSIONS: LncRNA FOXD2-AS1 promoted hepatocellular carcinoma development by regulation miR-185/AKT axis. International Scientific Literature, Inc. 2019-12-16 /pmc/articles/PMC6929557/ /pubmed/31841454 http://dx.doi.org/10.12659/MSM.918230 Text en © Med Sci Monit, 2019 This work is licensed under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0 (https://creativecommons.org/licenses/by-nc-nd/4.0/) )
spellingShingle Lab/In Vitro Research
Chen, Zheng
Zhang, Zhen
Zhao, Dongbo
Feng, Wei
Meng, Fanlai
Han, Shihui
Lin, Bin
Shi, Xin
Long Noncoding RNA (lncRNA) FOXD2-AS1 Promotes Cell Proliferation and Metastasis in Hepatocellular Carcinoma by Regulating MiR-185/AKT Axis
title Long Noncoding RNA (lncRNA) FOXD2-AS1 Promotes Cell Proliferation and Metastasis in Hepatocellular Carcinoma by Regulating MiR-185/AKT Axis
title_full Long Noncoding RNA (lncRNA) FOXD2-AS1 Promotes Cell Proliferation and Metastasis in Hepatocellular Carcinoma by Regulating MiR-185/AKT Axis
title_fullStr Long Noncoding RNA (lncRNA) FOXD2-AS1 Promotes Cell Proliferation and Metastasis in Hepatocellular Carcinoma by Regulating MiR-185/AKT Axis
title_full_unstemmed Long Noncoding RNA (lncRNA) FOXD2-AS1 Promotes Cell Proliferation and Metastasis in Hepatocellular Carcinoma by Regulating MiR-185/AKT Axis
title_short Long Noncoding RNA (lncRNA) FOXD2-AS1 Promotes Cell Proliferation and Metastasis in Hepatocellular Carcinoma by Regulating MiR-185/AKT Axis
title_sort long noncoding rna (lncrna) foxd2-as1 promotes cell proliferation and metastasis in hepatocellular carcinoma by regulating mir-185/akt axis
topic Lab/In Vitro Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929557/
https://www.ncbi.nlm.nih.gov/pubmed/31841454
http://dx.doi.org/10.12659/MSM.918230
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