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Adaptive amino acid substitutions enable transmission of an H9N2 avian influenza virus in guinea pigs

H9N2 is the most prevalent low pathogenic avian influenza virus (LPAIV) in domestic poultry in the world. Two distinct H9N2 poultry lineages, G1-like (A/quail/Hong Kong/G1/97) and Y280-like (A/Duck/Hong Kong/Y280/1997) viruses, are usually associated with binding affinity for both α 2,3 and α 2,6 si...

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Autores principales: Lina, Liu, Saijuan, Chen, Chengyu, Wang, Yuefeng, Lu, Shishan, Dong, Ligong, Chen, Kangkang, Guo, Zhendong, Guo, Jiakai, Li, Jianhui, Zhang, Qingping, Luo, Wenting, Zhang, Yu, Shang, Honglin, Wang, Tengfei, Zhang, Guoyuan, Wen, Jiping, Zhu, Chunmao, Zhang, Meilin, Jin, Yuwei, Gao, Huabin, Shao, Zongzheng, Zhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930279/
https://www.ncbi.nlm.nih.gov/pubmed/31875046
http://dx.doi.org/10.1038/s41598-019-56122-6
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author Lina, Liu
Saijuan, Chen
Chengyu, Wang
Yuefeng, Lu
Shishan, Dong
Ligong, Chen
Kangkang, Guo
Zhendong, Guo
Jiakai, Li
Jianhui, Zhang
Qingping, Luo
Wenting, Zhang
Yu, Shang
Honglin, Wang
Tengfei, Zhang
Guoyuan, Wen
Jiping, Zhu
Chunmao, Zhang
Meilin, Jin
Yuwei, Gao
Huabin, Shao
Zongzheng, Zhao
author_facet Lina, Liu
Saijuan, Chen
Chengyu, Wang
Yuefeng, Lu
Shishan, Dong
Ligong, Chen
Kangkang, Guo
Zhendong, Guo
Jiakai, Li
Jianhui, Zhang
Qingping, Luo
Wenting, Zhang
Yu, Shang
Honglin, Wang
Tengfei, Zhang
Guoyuan, Wen
Jiping, Zhu
Chunmao, Zhang
Meilin, Jin
Yuwei, Gao
Huabin, Shao
Zongzheng, Zhao
author_sort Lina, Liu
collection PubMed
description H9N2 is the most prevalent low pathogenic avian influenza virus (LPAIV) in domestic poultry in the world. Two distinct H9N2 poultry lineages, G1-like (A/quail/Hong Kong/G1/97) and Y280-like (A/Duck/Hong Kong/Y280/1997) viruses, are usually associated with binding affinity for both α 2,3 and α 2,6 sialic acid receptors (avian and human receptors), raising concern whether these viruses possess pandemic potential. To explore the impact of mouse adaptation on the transmissibility of a Y280-like virus A/Chicken/Hubei/214/2017(H9N2) (abbreviated as WT), we performed serial lung-to-lung passages of the WT virus in mice. The mouse-adapted variant (MA) exhibited enhanced pathogenicity and advantaged transmissibility after passaging in mice. Sequence analysis of the complete genomes of the MA virus revealed a total of 16 amino acid substitutions. These mutations distributed across 7 segments including PB2, PB1, PA, NP, HA, NA and NS1 genes. Furthermore, we generated a panel of recombinant or mutant H9N2 viruses using reverse genetics technology and confirmed that the PB2 gene governing the increased pathogenicity and transmissibility. The combinations of 340 K and 588 V in PB2 were important in determining the altered features. Our findings elucidate the specific mutations in PB2 contribute to the phenotype differences and emphasize the importance of monitoring the identified amino acid substitutions due to their potential threat to human health.
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spelling pubmed-69302792019-12-27 Adaptive amino acid substitutions enable transmission of an H9N2 avian influenza virus in guinea pigs Lina, Liu Saijuan, Chen Chengyu, Wang Yuefeng, Lu Shishan, Dong Ligong, Chen Kangkang, Guo Zhendong, Guo Jiakai, Li Jianhui, Zhang Qingping, Luo Wenting, Zhang Yu, Shang Honglin, Wang Tengfei, Zhang Guoyuan, Wen Jiping, Zhu Chunmao, Zhang Meilin, Jin Yuwei, Gao Huabin, Shao Zongzheng, Zhao Sci Rep Article H9N2 is the most prevalent low pathogenic avian influenza virus (LPAIV) in domestic poultry in the world. Two distinct H9N2 poultry lineages, G1-like (A/quail/Hong Kong/G1/97) and Y280-like (A/Duck/Hong Kong/Y280/1997) viruses, are usually associated with binding affinity for both α 2,3 and α 2,6 sialic acid receptors (avian and human receptors), raising concern whether these viruses possess pandemic potential. To explore the impact of mouse adaptation on the transmissibility of a Y280-like virus A/Chicken/Hubei/214/2017(H9N2) (abbreviated as WT), we performed serial lung-to-lung passages of the WT virus in mice. The mouse-adapted variant (MA) exhibited enhanced pathogenicity and advantaged transmissibility after passaging in mice. Sequence analysis of the complete genomes of the MA virus revealed a total of 16 amino acid substitutions. These mutations distributed across 7 segments including PB2, PB1, PA, NP, HA, NA and NS1 genes. Furthermore, we generated a panel of recombinant or mutant H9N2 viruses using reverse genetics technology and confirmed that the PB2 gene governing the increased pathogenicity and transmissibility. The combinations of 340 K and 588 V in PB2 were important in determining the altered features. Our findings elucidate the specific mutations in PB2 contribute to the phenotype differences and emphasize the importance of monitoring the identified amino acid substitutions due to their potential threat to human health. Nature Publishing Group UK 2019-12-24 /pmc/articles/PMC6930279/ /pubmed/31875046 http://dx.doi.org/10.1038/s41598-019-56122-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lina, Liu
Saijuan, Chen
Chengyu, Wang
Yuefeng, Lu
Shishan, Dong
Ligong, Chen
Kangkang, Guo
Zhendong, Guo
Jiakai, Li
Jianhui, Zhang
Qingping, Luo
Wenting, Zhang
Yu, Shang
Honglin, Wang
Tengfei, Zhang
Guoyuan, Wen
Jiping, Zhu
Chunmao, Zhang
Meilin, Jin
Yuwei, Gao
Huabin, Shao
Zongzheng, Zhao
Adaptive amino acid substitutions enable transmission of an H9N2 avian influenza virus in guinea pigs
title Adaptive amino acid substitutions enable transmission of an H9N2 avian influenza virus in guinea pigs
title_full Adaptive amino acid substitutions enable transmission of an H9N2 avian influenza virus in guinea pigs
title_fullStr Adaptive amino acid substitutions enable transmission of an H9N2 avian influenza virus in guinea pigs
title_full_unstemmed Adaptive amino acid substitutions enable transmission of an H9N2 avian influenza virus in guinea pigs
title_short Adaptive amino acid substitutions enable transmission of an H9N2 avian influenza virus in guinea pigs
title_sort adaptive amino acid substitutions enable transmission of an h9n2 avian influenza virus in guinea pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930279/
https://www.ncbi.nlm.nih.gov/pubmed/31875046
http://dx.doi.org/10.1038/s41598-019-56122-6
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