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Temporal development of the oral microbiome and prediction of early childhood caries

Human microbiomes are predicted to assemble in a reproducible and ordered manner yet there is limited knowledge on the development of the complex bacterial communities that constitute the oral microbiome. The oral microbiome plays major roles in many oral diseases including early childhood caries (E...

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Autores principales: Dashper, S. G., Mitchell, H. L., Lê Cao, K.-A., Carpenter, L., Gussy, M. G., Calache, H., Gladman, S. L., Bulach, D. M., Hoffmann, B., Catmull, D. V., Pruilh, S., Johnson, S., Gibbs, L., Amezdroz, E., Bhatnagar, U., Seemann, T., Mnatzaganian, G., Manton, D. J., Reynolds, E. C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930300/
https://www.ncbi.nlm.nih.gov/pubmed/31874981
http://dx.doi.org/10.1038/s41598-019-56233-0
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author Dashper, S. G.
Mitchell, H. L.
Lê Cao, K.-A.
Carpenter, L.
Gussy, M. G.
Calache, H.
Gladman, S. L.
Bulach, D. M.
Hoffmann, B.
Catmull, D. V.
Pruilh, S.
Johnson, S.
Gibbs, L.
Amezdroz, E.
Bhatnagar, U.
Seemann, T.
Mnatzaganian, G.
Manton, D. J.
Reynolds, E. C.
author_facet Dashper, S. G.
Mitchell, H. L.
Lê Cao, K.-A.
Carpenter, L.
Gussy, M. G.
Calache, H.
Gladman, S. L.
Bulach, D. M.
Hoffmann, B.
Catmull, D. V.
Pruilh, S.
Johnson, S.
Gibbs, L.
Amezdroz, E.
Bhatnagar, U.
Seemann, T.
Mnatzaganian, G.
Manton, D. J.
Reynolds, E. C.
author_sort Dashper, S. G.
collection PubMed
description Human microbiomes are predicted to assemble in a reproducible and ordered manner yet there is limited knowledge on the development of the complex bacterial communities that constitute the oral microbiome. The oral microbiome plays major roles in many oral diseases including early childhood caries (ECC), which afflicts up to 70% of children in some countries. Saliva contains oral bacteria that are indicative of the whole oral microbiome and may have the ability to reflect the dysbiosis in supragingival plaque communities that initiates the clinical manifestations of ECC. The aim of this study was to determine the assembly of the oral microbiome during the first four years of life and compare it with the clinical development of ECC. The oral microbiomes of 134 children enrolled in a birth cohort study were determined at six ages between two months and four years-of-age and their mother’s oral microbiome was determined at a single time point. We identified and quantified 356 operational taxonomic units (OTUs) of bacteria in saliva by sequencing the V4 region of the bacterial 16S RNA genes. Bacterial alpha diversity increased from a mean of 31 OTUs in the saliva of infants at 1.9 months-of-age to 84 OTUs at 39 months-of-age. The oral microbiome showed a distinct shift in composition as the children matured. The microbiome data were compared with the clinical development of ECC in the cohort at 39, 48, and 60 months-of-age as determined by ICDAS-II assessment. Streptococcus mutans was the most discriminatory oral bacterial species between health and current disease, with an increased abundance in disease. Overall our study demonstrates an ordered temporal development of the oral microbiome, describes a limited core oral microbiome and indicates that saliva testing of infants may help predict ECC risk.
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spelling pubmed-69303002019-12-27 Temporal development of the oral microbiome and prediction of early childhood caries Dashper, S. G. Mitchell, H. L. Lê Cao, K.-A. Carpenter, L. Gussy, M. G. Calache, H. Gladman, S. L. Bulach, D. M. Hoffmann, B. Catmull, D. V. Pruilh, S. Johnson, S. Gibbs, L. Amezdroz, E. Bhatnagar, U. Seemann, T. Mnatzaganian, G. Manton, D. J. Reynolds, E. C. Sci Rep Article Human microbiomes are predicted to assemble in a reproducible and ordered manner yet there is limited knowledge on the development of the complex bacterial communities that constitute the oral microbiome. The oral microbiome plays major roles in many oral diseases including early childhood caries (ECC), which afflicts up to 70% of children in some countries. Saliva contains oral bacteria that are indicative of the whole oral microbiome and may have the ability to reflect the dysbiosis in supragingival plaque communities that initiates the clinical manifestations of ECC. The aim of this study was to determine the assembly of the oral microbiome during the first four years of life and compare it with the clinical development of ECC. The oral microbiomes of 134 children enrolled in a birth cohort study were determined at six ages between two months and four years-of-age and their mother’s oral microbiome was determined at a single time point. We identified and quantified 356 operational taxonomic units (OTUs) of bacteria in saliva by sequencing the V4 region of the bacterial 16S RNA genes. Bacterial alpha diversity increased from a mean of 31 OTUs in the saliva of infants at 1.9 months-of-age to 84 OTUs at 39 months-of-age. The oral microbiome showed a distinct shift in composition as the children matured. The microbiome data were compared with the clinical development of ECC in the cohort at 39, 48, and 60 months-of-age as determined by ICDAS-II assessment. Streptococcus mutans was the most discriminatory oral bacterial species between health and current disease, with an increased abundance in disease. Overall our study demonstrates an ordered temporal development of the oral microbiome, describes a limited core oral microbiome and indicates that saliva testing of infants may help predict ECC risk. Nature Publishing Group UK 2019-12-24 /pmc/articles/PMC6930300/ /pubmed/31874981 http://dx.doi.org/10.1038/s41598-019-56233-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Dashper, S. G.
Mitchell, H. L.
Lê Cao, K.-A.
Carpenter, L.
Gussy, M. G.
Calache, H.
Gladman, S. L.
Bulach, D. M.
Hoffmann, B.
Catmull, D. V.
Pruilh, S.
Johnson, S.
Gibbs, L.
Amezdroz, E.
Bhatnagar, U.
Seemann, T.
Mnatzaganian, G.
Manton, D. J.
Reynolds, E. C.
Temporal development of the oral microbiome and prediction of early childhood caries
title Temporal development of the oral microbiome and prediction of early childhood caries
title_full Temporal development of the oral microbiome and prediction of early childhood caries
title_fullStr Temporal development of the oral microbiome and prediction of early childhood caries
title_full_unstemmed Temporal development of the oral microbiome and prediction of early childhood caries
title_short Temporal development of the oral microbiome and prediction of early childhood caries
title_sort temporal development of the oral microbiome and prediction of early childhood caries
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930300/
https://www.ncbi.nlm.nih.gov/pubmed/31874981
http://dx.doi.org/10.1038/s41598-019-56233-0
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