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LINC00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma

Background: Proverbially, the incidence rate of papillary thyroid carcinoma (PTC) has increased year by year. Many long noncoding RNAs (lncRNAs) have been discovered having a relationship with tumor genesis tightly recently. Thanks to the previous researches, we found long intergenic noncoding RNA 0...

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Autores principales: Xiang, Jingjing, Guan, Yaoyao, Bhandari, Adheesh, Xia, Erjie, Wen, Jialiang, Wang, Ouchen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930424/
https://www.ncbi.nlm.nih.gov/pubmed/31897240
http://dx.doi.org/10.7150/jca.35364
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author Xiang, Jingjing
Guan, Yaoyao
Bhandari, Adheesh
Xia, Erjie
Wen, Jialiang
Wang, Ouchen
author_facet Xiang, Jingjing
Guan, Yaoyao
Bhandari, Adheesh
Xia, Erjie
Wen, Jialiang
Wang, Ouchen
author_sort Xiang, Jingjing
collection PubMed
description Background: Proverbially, the incidence rate of papillary thyroid carcinoma (PTC) has increased year by year. Many long noncoding RNAs (lncRNAs) have been discovered having a relationship with tumor genesis tightly recently. Thanks to the previous researches, we found long intergenic noncoding RNA 00511 (LINC00511) is overexpressed and acts as an oncogene in non-small-cell lung cancer and breast cancer. However, the biological role and function of LINC00511 are still unclear in PTC. Methods: We got the expression of LINC00511 in PTC tissues and matched adjacent tissues, as well the cell lines (B-CPAP, KTC-1, and KTC-1) by way of quantitative real-time polymerase chain reaction (qRT-PCR). In vitro, we knocked down the LINC00511 with small interfering RNA in PTC cell lines and demonstrated the function of LINC00511 by Cell Counting Kit-8, cell colony formation, Transwell migration, Transwell invasion, apoptosis assays, and cell cycle assays. Then, we discovered several downstream proteins of LINC00511 using Western blotting. Results: We proved that LINC00511's expression in PTC tissues and cell lines is higher than the control. LINC00511 promoted cellular proliferation, migration, invasion, G1/S transition and reduced apoptosis in vitro experiment. Knocked-down of LINC00511 resulted in the reduction of histone methyltransferase enhancer of zeste homolog 2 (EZH2), cyclin-dependent kinase 2 (CDK2) and cyclin-dependent kinase 4 (CDK4). Conclusions: Our results certified the role and function of LINC00511 in PTC, and it could become a novel tumor therapeutic target.
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spelling pubmed-69304242020-01-03 LINC00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma Xiang, Jingjing Guan, Yaoyao Bhandari, Adheesh Xia, Erjie Wen, Jialiang Wang, Ouchen J Cancer Research Paper Background: Proverbially, the incidence rate of papillary thyroid carcinoma (PTC) has increased year by year. Many long noncoding RNAs (lncRNAs) have been discovered having a relationship with tumor genesis tightly recently. Thanks to the previous researches, we found long intergenic noncoding RNA 00511 (LINC00511) is overexpressed and acts as an oncogene in non-small-cell lung cancer and breast cancer. However, the biological role and function of LINC00511 are still unclear in PTC. Methods: We got the expression of LINC00511 in PTC tissues and matched adjacent tissues, as well the cell lines (B-CPAP, KTC-1, and KTC-1) by way of quantitative real-time polymerase chain reaction (qRT-PCR). In vitro, we knocked down the LINC00511 with small interfering RNA in PTC cell lines and demonstrated the function of LINC00511 by Cell Counting Kit-8, cell colony formation, Transwell migration, Transwell invasion, apoptosis assays, and cell cycle assays. Then, we discovered several downstream proteins of LINC00511 using Western blotting. Results: We proved that LINC00511's expression in PTC tissues and cell lines is higher than the control. LINC00511 promoted cellular proliferation, migration, invasion, G1/S transition and reduced apoptosis in vitro experiment. Knocked-down of LINC00511 resulted in the reduction of histone methyltransferase enhancer of zeste homolog 2 (EZH2), cyclin-dependent kinase 2 (CDK2) and cyclin-dependent kinase 4 (CDK4). Conclusions: Our results certified the role and function of LINC00511 in PTC, and it could become a novel tumor therapeutic target. Ivyspring International Publisher 2020-01-01 /pmc/articles/PMC6930424/ /pubmed/31897240 http://dx.doi.org/10.7150/jca.35364 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Xiang, Jingjing
Guan, Yaoyao
Bhandari, Adheesh
Xia, Erjie
Wen, Jialiang
Wang, Ouchen
LINC00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma
title LINC00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma
title_full LINC00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma
title_fullStr LINC00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma
title_full_unstemmed LINC00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma
title_short LINC00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma
title_sort linc00511 influences cellular proliferation through cyclin-dependent kinases in papillary thyroid carcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930424/
https://www.ncbi.nlm.nih.gov/pubmed/31897240
http://dx.doi.org/10.7150/jca.35364
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