Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water

Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from Thermomyces lanuginosus is used to hydrolyze xylan...

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Detalles Bibliográficos
Autores principales: Ostadjoo, Shaghayegh, Hammerer, Fabien, Dietrich, Karolin, Dumont, Marie-Josée, Friscic, Tomislav, Auclair, Karine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930478/
https://www.ncbi.nlm.nih.gov/pubmed/31756935
http://dx.doi.org/10.3390/molecules24234206
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author Ostadjoo, Shaghayegh
Hammerer, Fabien
Dietrich, Karolin
Dumont, Marie-Josée
Friscic, Tomislav
Auclair, Karine
author_facet Ostadjoo, Shaghayegh
Hammerer, Fabien
Dietrich, Karolin
Dumont, Marie-Josée
Friscic, Tomislav
Auclair, Karine
author_sort Ostadjoo, Shaghayegh
collection PubMed
description Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from Thermomyces lanuginosus is used to hydrolyze xylans from different sources. We report an innovative enzymatic process which avoids the use of bulk aqueous, organic or inorganic solvent, and enables hydrolysis of hemicellulose directly from chemically untreated biomass, to low-weight, soluble oligoxylosaccharides in >70% yields.
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spelling pubmed-69304782019-12-26 Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water Ostadjoo, Shaghayegh Hammerer, Fabien Dietrich, Karolin Dumont, Marie-Josée Friscic, Tomislav Auclair, Karine Molecules Article Current enzymatic methods for hemicellulosic biomass depolymerization are solution-based, typically require a harsh chemical pre-treatment of the material and large volumes of water, yet lack in efficiency. In our study, xylanase (E.C. 3.2.1.8) from Thermomyces lanuginosus is used to hydrolyze xylans from different sources. We report an innovative enzymatic process which avoids the use of bulk aqueous, organic or inorganic solvent, and enables hydrolysis of hemicellulose directly from chemically untreated biomass, to low-weight, soluble oligoxylosaccharides in >70% yields. MDPI 2019-11-20 /pmc/articles/PMC6930478/ /pubmed/31756935 http://dx.doi.org/10.3390/molecules24234206 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ostadjoo, Shaghayegh
Hammerer, Fabien
Dietrich, Karolin
Dumont, Marie-Josée
Friscic, Tomislav
Auclair, Karine
Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water
title Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water
title_full Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water
title_fullStr Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water
title_full_unstemmed Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water
title_short Efficient Enzymatic Hydrolysis of Biomass Hemicellulose in the Absence of Bulk Water
title_sort efficient enzymatic hydrolysis of biomass hemicellulose in the absence of bulk water
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930478/
https://www.ncbi.nlm.nih.gov/pubmed/31756935
http://dx.doi.org/10.3390/molecules24234206
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