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Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes

BACKGROUND: Type 2 diabetes, or T2D, is a metabolic disease that results in insulin resistance. In the present study, we hypothesize that metabolomic analysis in blood samples of T2D patients sharing the same ethnic background can recover new metabolic biomarkers and pathways that elucidate early di...

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Autores principales: Al-Aama, Jumana Y, Al Mahdi, Hadiah B, Salama, Mohammed A, Bakur, Khadija H, Alhozali, Amani, Mosli, Hala H, Bahijri, Suhad M, Bahieldin, Ahmed, Willmitzer, Lothar, Edris, Sherif
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930579/
https://www.ncbi.nlm.nih.gov/pubmed/31908508
http://dx.doi.org/10.2147/DMSO.S215528
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author Al-Aama, Jumana Y
Al Mahdi, Hadiah B
Salama, Mohammed A
Bakur, Khadija H
Alhozali, Amani
Mosli, Hala H
Bahijri, Suhad M
Bahieldin, Ahmed
Willmitzer, Lothar
Edris, Sherif
author_facet Al-Aama, Jumana Y
Al Mahdi, Hadiah B
Salama, Mohammed A
Bakur, Khadija H
Alhozali, Amani
Mosli, Hala H
Bahijri, Suhad M
Bahieldin, Ahmed
Willmitzer, Lothar
Edris, Sherif
author_sort Al-Aama, Jumana Y
collection PubMed
description BACKGROUND: Type 2 diabetes, or T2D, is a metabolic disease that results in insulin resistance. In the present study, we hypothesize that metabolomic analysis in blood samples of T2D patients sharing the same ethnic background can recover new metabolic biomarkers and pathways that elucidate early diagnosis and predict the incidence of T2D. METHODS: The study included 34 T2D patients and 33 healthy volunteers recruited between the years 2012 and 2013; the secondary metabolites were extracted from blood samples and analyzed using HPLC. RESULTS: Principal coordinate analysis and hierarchical clustering patterns for the uncharacterized negatively and positively charged metabolites indicated that samples from healthy individuals and T2D patients were largely separated with only a few exceptions. The inspection of the top 10% secondary metabolites indicated an increase in fucose, tryptophan and choline levels in the T2D patients, while there was a reduction in carnitine, homoserine, allothreonine, serine and betaine as compared to healthy individuals. These metabolites participate mainly in three cross-talking pathways, namely “glucagon signaling”, “glycine, serine and threonine” and “bile secretion”. Reduced level of carnitine in T2D patients is known to participate in the impaired insulin-stimulated glucose utilization, while reduced betaine level in T2D patients is known as a common feature of this metabolic syndrome and can result in the reduced glycine production and the occurrence of insulin resistance. However, reduced levels of serine, homoserine and allothrionine, substrates for glycine production, indicate the depletion of glycine, thus possibly impair insulin sensitivity in T2D patients of the present study. CONCLUSION: We introduce serine, homoserine and allothrionine as new potential biomarkers of T2D.
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spelling pubmed-69305792020-01-06 Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes Al-Aama, Jumana Y Al Mahdi, Hadiah B Salama, Mohammed A Bakur, Khadija H Alhozali, Amani Mosli, Hala H Bahijri, Suhad M Bahieldin, Ahmed Willmitzer, Lothar Edris, Sherif Diabetes Metab Syndr Obes Original Research BACKGROUND: Type 2 diabetes, or T2D, is a metabolic disease that results in insulin resistance. In the present study, we hypothesize that metabolomic analysis in blood samples of T2D patients sharing the same ethnic background can recover new metabolic biomarkers and pathways that elucidate early diagnosis and predict the incidence of T2D. METHODS: The study included 34 T2D patients and 33 healthy volunteers recruited between the years 2012 and 2013; the secondary metabolites were extracted from blood samples and analyzed using HPLC. RESULTS: Principal coordinate analysis and hierarchical clustering patterns for the uncharacterized negatively and positively charged metabolites indicated that samples from healthy individuals and T2D patients were largely separated with only a few exceptions. The inspection of the top 10% secondary metabolites indicated an increase in fucose, tryptophan and choline levels in the T2D patients, while there was a reduction in carnitine, homoserine, allothreonine, serine and betaine as compared to healthy individuals. These metabolites participate mainly in three cross-talking pathways, namely “glucagon signaling”, “glycine, serine and threonine” and “bile secretion”. Reduced level of carnitine in T2D patients is known to participate in the impaired insulin-stimulated glucose utilization, while reduced betaine level in T2D patients is known as a common feature of this metabolic syndrome and can result in the reduced glycine production and the occurrence of insulin resistance. However, reduced levels of serine, homoserine and allothrionine, substrates for glycine production, indicate the depletion of glycine, thus possibly impair insulin sensitivity in T2D patients of the present study. CONCLUSION: We introduce serine, homoserine and allothrionine as new potential biomarkers of T2D. Dove 2019-12-17 /pmc/articles/PMC6930579/ /pubmed/31908508 http://dx.doi.org/10.2147/DMSO.S215528 Text en © 2019 Al-Aama et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Al-Aama, Jumana Y
Al Mahdi, Hadiah B
Salama, Mohammed A
Bakur, Khadija H
Alhozali, Amani
Mosli, Hala H
Bahijri, Suhad M
Bahieldin, Ahmed
Willmitzer, Lothar
Edris, Sherif
Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title_full Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title_fullStr Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title_full_unstemmed Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title_short Detection of Secondary Metabolites as Biomarkers for the Early Diagnosis and Prevention of Type 2 Diabetes
title_sort detection of secondary metabolites as biomarkers for the early diagnosis and prevention of type 2 diabetes
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930579/
https://www.ncbi.nlm.nih.gov/pubmed/31908508
http://dx.doi.org/10.2147/DMSO.S215528
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