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Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease

Aims: Kawasaki disease is an acute pediatric vasculitis whose etiology remains unknown but epidemiology and clinical presentation suggest a viral etiology. We performed unbiased high-throughput-sequencing on blood of patients with Kawasaki Disease (KD). Materials and Methods: High-throughput-sequenc...

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Autores principales: L'Huillier, Arnaud G., Brito, Francisco, Wagner, Noemie, Cordey, Samuel, Zdobnov, Evgeny, Posfay-Barbe, Klara M., Kaiser, Laurent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930886/
https://www.ncbi.nlm.nih.gov/pubmed/31921732
http://dx.doi.org/10.3389/fped.2019.00524
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author L'Huillier, Arnaud G.
Brito, Francisco
Wagner, Noemie
Cordey, Samuel
Zdobnov, Evgeny
Posfay-Barbe, Klara M.
Kaiser, Laurent
author_facet L'Huillier, Arnaud G.
Brito, Francisco
Wagner, Noemie
Cordey, Samuel
Zdobnov, Evgeny
Posfay-Barbe, Klara M.
Kaiser, Laurent
author_sort L'Huillier, Arnaud G.
collection PubMed
description Aims: Kawasaki disease is an acute pediatric vasculitis whose etiology remains unknown but epidemiology and clinical presentation suggest a viral etiology. We performed unbiased high-throughput-sequencing on blood of patients with Kawasaki Disease (KD). Materials and Methods: High-throughput-sequencing was performed directly on blood of children with typical KD. Sequences were aligned against a database of clinically relevant viruses. Results: Four patients were acutely infected in the blood, with respectively, poliovirus (vaccine strain), measles (vaccine strain), rhinovirus and bocavirus. Patients with poliovirus and measles had received oral polio and measles vaccines, respectively, twelve and 2 weeks prior. Conclusion: Viral signatures were identified in more than half of the patients, including some corresponding to their vaccinal history. This could suggest a temporal association with KD.
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spelling pubmed-69308862020-01-09 Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease L'Huillier, Arnaud G. Brito, Francisco Wagner, Noemie Cordey, Samuel Zdobnov, Evgeny Posfay-Barbe, Klara M. Kaiser, Laurent Front Pediatr Pediatrics Aims: Kawasaki disease is an acute pediatric vasculitis whose etiology remains unknown but epidemiology and clinical presentation suggest a viral etiology. We performed unbiased high-throughput-sequencing on blood of patients with Kawasaki Disease (KD). Materials and Methods: High-throughput-sequencing was performed directly on blood of children with typical KD. Sequences were aligned against a database of clinically relevant viruses. Results: Four patients were acutely infected in the blood, with respectively, poliovirus (vaccine strain), measles (vaccine strain), rhinovirus and bocavirus. Patients with poliovirus and measles had received oral polio and measles vaccines, respectively, twelve and 2 weeks prior. Conclusion: Viral signatures were identified in more than half of the patients, including some corresponding to their vaccinal history. This could suggest a temporal association with KD. Frontiers Media S.A. 2019-12-19 /pmc/articles/PMC6930886/ /pubmed/31921732 http://dx.doi.org/10.3389/fped.2019.00524 Text en Copyright © 2019 L'Huillier, Brito, Wagner, Cordey, Zdobnov, Posfay-Barbe and Kaiser. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pediatrics
L'Huillier, Arnaud G.
Brito, Francisco
Wagner, Noemie
Cordey, Samuel
Zdobnov, Evgeny
Posfay-Barbe, Klara M.
Kaiser, Laurent
Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease
title Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease
title_full Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease
title_fullStr Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease
title_full_unstemmed Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease
title_short Identification of Viral Signatures Using High-Throughput Sequencing on Blood of Patients With Kawasaki Disease
title_sort identification of viral signatures using high-throughput sequencing on blood of patients with kawasaki disease
topic Pediatrics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930886/
https://www.ncbi.nlm.nih.gov/pubmed/31921732
http://dx.doi.org/10.3389/fped.2019.00524
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