Cargando…
Quantitative examination of the inhibitory activation of molecular targeting agents in hepatocellular carcinoma patient‐derived cell invasion via a novel in vivo tumor model
BACKGROUND: The outcomes for patients with advanced hepatocellular carcinoma (HCC) receiving sorafenib are far from satisfactory because of treatment resistance to sorafenib. However, the exact mechanism of resistance to sorafenib remains unclear and it is valuable to establish a novel mouse model t...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930997/ https://www.ncbi.nlm.nih.gov/pubmed/31942558 http://dx.doi.org/10.1002/ame2.12085 |
_version_ | 1783483015300644864 |
---|---|
author | Sun, Huiwei Feng, Fan Xie, Hui Li, Xiaojuan Jiang, Qiyu Chai, Yantao Wang, Zhijie Yang, Ruichuang Li, Ruisheng Hou, Jun |
author_facet | Sun, Huiwei Feng, Fan Xie, Hui Li, Xiaojuan Jiang, Qiyu Chai, Yantao Wang, Zhijie Yang, Ruichuang Li, Ruisheng Hou, Jun |
author_sort | Sun, Huiwei |
collection | PubMed |
description | BACKGROUND: The outcomes for patients with advanced hepatocellular carcinoma (HCC) receiving sorafenib are far from satisfactory because of treatment resistance to sorafenib. However, the exact mechanism of resistance to sorafenib remains unclear and it is valuable to establish a novel mouse model to quantitatively analyze the inhibition rates of sorafenib on the invasive growth of HCC cells in the liver. METHODS: HCC tissue microblocks derived from patients were cultured and mixed with hydrogel drops. Then, hydrogel drops containing microblocks of HCC tissue were attached onto the surface of the livers of nude mice to form lesions or nodules of HCC. The mice received molecular targeting agents through oral administration. Livers with tumor nodules were harvested for H&E staining (hematoxylin‐eosin staining) analysis and H&E staining images were quantitatively analyzed using image J software. The invasive growth of HCC cells into the liver was calculated using the depth of the lesions compared with the total thickness of the liver. RESULTS: Microblocks containing cells derived from HCC patients can form lesions in the liver of nude mice. Oral administration of molecular targeting agents inhibited the invasive growth of HCC cells in the liver of nude mice. CONCLUSIONS: The model established in this study involves the invasive growth of HCC cells in the liver of nude mice, and the model allows for the quantitative analysis of the inhibitory effect of molecular targeting agents on the invasion of HCC cells in vivo. |
format | Online Article Text |
id | pubmed-6930997 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69309972020-01-15 Quantitative examination of the inhibitory activation of molecular targeting agents in hepatocellular carcinoma patient‐derived cell invasion via a novel in vivo tumor model Sun, Huiwei Feng, Fan Xie, Hui Li, Xiaojuan Jiang, Qiyu Chai, Yantao Wang, Zhijie Yang, Ruichuang Li, Ruisheng Hou, Jun Animal Model Exp Med Original Articles BACKGROUND: The outcomes for patients with advanced hepatocellular carcinoma (HCC) receiving sorafenib are far from satisfactory because of treatment resistance to sorafenib. However, the exact mechanism of resistance to sorafenib remains unclear and it is valuable to establish a novel mouse model to quantitatively analyze the inhibition rates of sorafenib on the invasive growth of HCC cells in the liver. METHODS: HCC tissue microblocks derived from patients were cultured and mixed with hydrogel drops. Then, hydrogel drops containing microblocks of HCC tissue were attached onto the surface of the livers of nude mice to form lesions or nodules of HCC. The mice received molecular targeting agents through oral administration. Livers with tumor nodules were harvested for H&E staining (hematoxylin‐eosin staining) analysis and H&E staining images were quantitatively analyzed using image J software. The invasive growth of HCC cells into the liver was calculated using the depth of the lesions compared with the total thickness of the liver. RESULTS: Microblocks containing cells derived from HCC patients can form lesions in the liver of nude mice. Oral administration of molecular targeting agents inhibited the invasive growth of HCC cells in the liver of nude mice. CONCLUSIONS: The model established in this study involves the invasive growth of HCC cells in the liver of nude mice, and the model allows for the quantitative analysis of the inhibitory effect of molecular targeting agents on the invasion of HCC cells in vivo. John Wiley and Sons Inc. 2019-09-27 /pmc/articles/PMC6930997/ /pubmed/31942558 http://dx.doi.org/10.1002/ame2.12085 Text en © 2019 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Sun, Huiwei Feng, Fan Xie, Hui Li, Xiaojuan Jiang, Qiyu Chai, Yantao Wang, Zhijie Yang, Ruichuang Li, Ruisheng Hou, Jun Quantitative examination of the inhibitory activation of molecular targeting agents in hepatocellular carcinoma patient‐derived cell invasion via a novel in vivo tumor model |
title | Quantitative examination of the inhibitory activation of molecular targeting agents in hepatocellular carcinoma patient‐derived cell invasion via a novel in vivo tumor model |
title_full | Quantitative examination of the inhibitory activation of molecular targeting agents in hepatocellular carcinoma patient‐derived cell invasion via a novel in vivo tumor model |
title_fullStr | Quantitative examination of the inhibitory activation of molecular targeting agents in hepatocellular carcinoma patient‐derived cell invasion via a novel in vivo tumor model |
title_full_unstemmed | Quantitative examination of the inhibitory activation of molecular targeting agents in hepatocellular carcinoma patient‐derived cell invasion via a novel in vivo tumor model |
title_short | Quantitative examination of the inhibitory activation of molecular targeting agents in hepatocellular carcinoma patient‐derived cell invasion via a novel in vivo tumor model |
title_sort | quantitative examination of the inhibitory activation of molecular targeting agents in hepatocellular carcinoma patient‐derived cell invasion via a novel in vivo tumor model |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930997/ https://www.ncbi.nlm.nih.gov/pubmed/31942558 http://dx.doi.org/10.1002/ame2.12085 |
work_keys_str_mv | AT sunhuiwei quantitativeexaminationoftheinhibitoryactivationofmoleculartargetingagentsinhepatocellularcarcinomapatientderivedcellinvasionviaanovelinvivotumormodel AT fengfan quantitativeexaminationoftheinhibitoryactivationofmoleculartargetingagentsinhepatocellularcarcinomapatientderivedcellinvasionviaanovelinvivotumormodel AT xiehui quantitativeexaminationoftheinhibitoryactivationofmoleculartargetingagentsinhepatocellularcarcinomapatientderivedcellinvasionviaanovelinvivotumormodel AT lixiaojuan quantitativeexaminationoftheinhibitoryactivationofmoleculartargetingagentsinhepatocellularcarcinomapatientderivedcellinvasionviaanovelinvivotumormodel AT jiangqiyu quantitativeexaminationoftheinhibitoryactivationofmoleculartargetingagentsinhepatocellularcarcinomapatientderivedcellinvasionviaanovelinvivotumormodel AT chaiyantao quantitativeexaminationoftheinhibitoryactivationofmoleculartargetingagentsinhepatocellularcarcinomapatientderivedcellinvasionviaanovelinvivotumormodel AT wangzhijie quantitativeexaminationoftheinhibitoryactivationofmoleculartargetingagentsinhepatocellularcarcinomapatientderivedcellinvasionviaanovelinvivotumormodel AT yangruichuang quantitativeexaminationoftheinhibitoryactivationofmoleculartargetingagentsinhepatocellularcarcinomapatientderivedcellinvasionviaanovelinvivotumormodel AT liruisheng quantitativeexaminationoftheinhibitoryactivationofmoleculartargetingagentsinhepatocellularcarcinomapatientderivedcellinvasionviaanovelinvivotumormodel AT houjun quantitativeexaminationoftheinhibitoryactivationofmoleculartargetingagentsinhepatocellularcarcinomapatientderivedcellinvasionviaanovelinvivotumormodel |