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Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MS(e)
Boswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties. A chromatographic method was developed for the analysis and quantification of six boswellic acid marker compounds, i.e., keto boswellic acid (1), 3-O-Acetyl 11-keto β-boswe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Xi'an Jiaotong University
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6931071/ https://www.ncbi.nlm.nih.gov/pubmed/31890341 http://dx.doi.org/10.1016/j.jpha.2019.09.007 |
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author | Katragunta, Kumar Siva, Bandi Kondepudi, Niharika Vadaparthi, P.R. Rao Rama Rao, Nadendla Tiwari, Ashok Kumar Suresh Babu, Katragadda |
author_facet | Katragunta, Kumar Siva, Bandi Kondepudi, Niharika Vadaparthi, P.R. Rao Rama Rao, Nadendla Tiwari, Ashok Kumar Suresh Babu, Katragadda |
author_sort | Katragunta, Kumar |
collection | PubMed |
description | Boswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties. A chromatographic method was developed for the analysis and quantification of six boswellic acid marker compounds, i.e., keto boswellic acid (1), 3-O-Acetyl 11-keto β-boswellic acid (2), ɑ-Boswellic acid (3), β-Boswellic acid (4), 3-O-Acetyl-ɑ-boswellic acid (5) and 3-O-Acetyl-β-boswellic acid (6) in commercial herbal products containing B. serrata as an ingredient. Combining UPLC with Q-Tof-MS/MS makes the better identification of secondary metabolites and adulterants in the herbal formulations containing B. serrata in rapid time using fragmentation approach than the traditional approaches. In this study quantification of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guidelines. Furthermore, minor phytochemical constituents were identified and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in B. serrata oleo-gum-resin extract were identified. |
format | Online Article Text |
id | pubmed-6931071 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Xi'an Jiaotong University |
record_format | MEDLINE/PubMed |
spelling | pubmed-69310712019-12-30 Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MS(e) Katragunta, Kumar Siva, Bandi Kondepudi, Niharika Vadaparthi, P.R. Rao Rama Rao, Nadendla Tiwari, Ashok Kumar Suresh Babu, Katragadda J Pharm Anal Original Article Boswellia serrata is a widely used herb in Indian systems of medicine and is well known for its potential medicinal properties. A chromatographic method was developed for the analysis and quantification of six boswellic acid marker compounds, i.e., keto boswellic acid (1), 3-O-Acetyl 11-keto β-boswellic acid (2), ɑ-Boswellic acid (3), β-Boswellic acid (4), 3-O-Acetyl-ɑ-boswellic acid (5) and 3-O-Acetyl-β-boswellic acid (6) in commercial herbal products containing B. serrata as an ingredient. Combining UPLC with Q-Tof-MS/MS makes the better identification of secondary metabolites and adulterants in the herbal formulations containing B. serrata in rapid time using fragmentation approach than the traditional approaches. In this study quantification of boswellic acids with UPLC-PDA method was performed as per the pharmacopeia guidelines. Furthermore, minor phytochemical constituents were identified and characterized with the help of LC-Q-Tof-MS/MS fragmentation data and various isoforms of boswellic acids and tirucallic acids in B. serrata oleo-gum-resin extract were identified. Xi'an Jiaotong University 2019-12 2019-10-01 /pmc/articles/PMC6931071/ /pubmed/31890341 http://dx.doi.org/10.1016/j.jpha.2019.09.007 Text en © 2019 Xi'an Jiaotong University. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Katragunta, Kumar Siva, Bandi Kondepudi, Niharika Vadaparthi, P.R. Rao Rama Rao, Nadendla Tiwari, Ashok Kumar Suresh Babu, Katragadda Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MS(e) |
title | Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MS(e) |
title_full | Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MS(e) |
title_fullStr | Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MS(e) |
title_full_unstemmed | Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MS(e) |
title_short | Estimation of boswellic acids in herbal formulations containing Boswellia serrata extract and comprehensive characterization of secondary metabolites using UPLC-Q-Tof-MS(e) |
title_sort | estimation of boswellic acids in herbal formulations containing boswellia serrata extract and comprehensive characterization of secondary metabolites using uplc-q-tof-ms(e) |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6931071/ https://www.ncbi.nlm.nih.gov/pubmed/31890341 http://dx.doi.org/10.1016/j.jpha.2019.09.007 |
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