Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag

Data in this article are associated with the research article “High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag” (Li et al., 2019) [1]. Data are provided on the development of a system for high-throughput (HTP) screening of mutants through the compa...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Yaping, Chong, Huimin, Zhang, Xiang, Yang, Xiaolan, Liao, Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Biochemistry, Genetics and Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6931123/
https://www.ncbi.nlm.nih.gov/pubmed/31890819
http://dx.doi.org/10.1016/j.dib.2019.104985
_version_ 1783483038817058816
author Li, Yaping
Chong, Huimin
Zhang, Xiang
Yang, Xiaolan
Liao, Fei
author_facet Li, Yaping
Chong, Huimin
Zhang, Xiang
Yang, Xiaolan
Liao, Fei
author_sort Li, Yaping
collection PubMed
description Data in this article are associated with the research article “High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag” (Li et al., 2019) [1]. Data are provided on the development of a system for high-throughput (HTP) screening of mutants through the comparison of the activity ratios of an applicable enzyme and its mutants to a suitable tag enzyme in cell lysates of their fused forms, with Escherichia coli alkaline phosphatase (ECAP) as the tag fused to the N-terminus of Pseudomonas Aeruginosa arylsulfatase (PAAS) and its mutants via a flexible linker. Data were made publicly available for further analyses.
format Online
Article
Text
id pubmed-6931123
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-69311232019-12-30 Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag Li, Yaping Chong, Huimin Zhang, Xiang Yang, Xiaolan Liao, Fei Data Brief Biochemistry, Genetics and Molecular Biology Data in this article are associated with the research article “High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag” (Li et al., 2019) [1]. Data are provided on the development of a system for high-throughput (HTP) screening of mutants through the comparison of the activity ratios of an applicable enzyme and its mutants to a suitable tag enzyme in cell lysates of their fused forms, with Escherichia coli alkaline phosphatase (ECAP) as the tag fused to the N-terminus of Pseudomonas Aeruginosa arylsulfatase (PAAS) and its mutants via a flexible linker. Data were made publicly available for further analyses. Elsevier 2019-12-13 /pmc/articles/PMC6931123/ /pubmed/31890819 http://dx.doi.org/10.1016/j.dib.2019.104985 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Li, Yaping
Chong, Huimin
Zhang, Xiang
Yang, Xiaolan
Liao, Fei
Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag
title Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag
title_full Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag
title_fullStr Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag
title_full_unstemmed Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag
title_short Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag
title_sort data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6931123/
https://www.ncbi.nlm.nih.gov/pubmed/31890819
http://dx.doi.org/10.1016/j.dib.2019.104985
work_keys_str_mv AT liyaping dataforhighthroughputscreeningofenzymemutantsbycomparisonoftheiractivityratiostoanenzymetag
AT chonghuimin dataforhighthroughputscreeningofenzymemutantsbycomparisonoftheiractivityratiostoanenzymetag
AT zhangxiang dataforhighthroughputscreeningofenzymemutantsbycomparisonoftheiractivityratiostoanenzymetag
AT yangxiaolan dataforhighthroughputscreeningofenzymemutantsbycomparisonoftheiractivityratiostoanenzymetag
AT liaofei dataforhighthroughputscreeningofenzymemutantsbycomparisonoftheiractivityratiostoanenzymetag

Ejemplares similares