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Detection of white head symptoms of panicle blast caused by Pyricularia oryzae using cut-flower dye

BACKGROUND: Breeding of rice with panicle resistance to rice blast disease caused by Pyricularia oryzae is a challenge towards sustainable rice production. Methods for accurate estimation of disease severity can support breeding. White head symptoms are a commonly used index of panicle blast in the...

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Autores principales: Hayashi, Keiko, Yoshida, Tomofumi, Hayano-Saito, Yuriko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6931245/
https://www.ncbi.nlm.nih.gov/pubmed/31889983
http://dx.doi.org/10.1186/s13007-019-0548-z
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author Hayashi, Keiko
Yoshida, Tomofumi
Hayano-Saito, Yuriko
author_facet Hayashi, Keiko
Yoshida, Tomofumi
Hayano-Saito, Yuriko
author_sort Hayashi, Keiko
collection PubMed
description BACKGROUND: Breeding of rice with panicle resistance to rice blast disease caused by Pyricularia oryzae is a challenge towards sustainable rice production. Methods for accurate estimation of disease severity can support breeding. White head symptoms are a commonly used index of panicle blast in the field. As the development mechanism of this symptom remains unclear, we used cut-flower dye (CFD) solution to visualize the infected panicle tissues. RESULTS: CFD delineated the edge of white head symptoms in rice panicles artificially infected with P. oryzae. Hyphae within the tissues were confirmed through staining with a fluorescent wheat germ agglutinin conjugate. Hyphal density was obviously diminished at the dye edge. Growing hyphae preferred to move along the vascular bundles; infected tissues lost the ability to transport water, leading to white head formation. By marking the edge of the white heads, this simple dyeing technique precisely reveals the extent of infection. Further, digital imaging allowed dried samples to be stored and reassessed later. CONCLUSIONS: The CFD detection technique served as a powerful tool for estimating disease severity by color, as it clearly revealed lesions in both the panicles and leaves. Combined with reliable methods for artificial inoculation and observation of infecting hyphae, this technique will advance the research and breeding of panicle blast-resistant rice.
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spelling pubmed-69312452019-12-30 Detection of white head symptoms of panicle blast caused by Pyricularia oryzae using cut-flower dye Hayashi, Keiko Yoshida, Tomofumi Hayano-Saito, Yuriko Plant Methods Research BACKGROUND: Breeding of rice with panicle resistance to rice blast disease caused by Pyricularia oryzae is a challenge towards sustainable rice production. Methods for accurate estimation of disease severity can support breeding. White head symptoms are a commonly used index of panicle blast in the field. As the development mechanism of this symptom remains unclear, we used cut-flower dye (CFD) solution to visualize the infected panicle tissues. RESULTS: CFD delineated the edge of white head symptoms in rice panicles artificially infected with P. oryzae. Hyphae within the tissues were confirmed through staining with a fluorescent wheat germ agglutinin conjugate. Hyphal density was obviously diminished at the dye edge. Growing hyphae preferred to move along the vascular bundles; infected tissues lost the ability to transport water, leading to white head formation. By marking the edge of the white heads, this simple dyeing technique precisely reveals the extent of infection. Further, digital imaging allowed dried samples to be stored and reassessed later. CONCLUSIONS: The CFD detection technique served as a powerful tool for estimating disease severity by color, as it clearly revealed lesions in both the panicles and leaves. Combined with reliable methods for artificial inoculation and observation of infecting hyphae, this technique will advance the research and breeding of panicle blast-resistant rice. BioMed Central 2019-12-26 /pmc/articles/PMC6931245/ /pubmed/31889983 http://dx.doi.org/10.1186/s13007-019-0548-z Text en © The Author(s) 2019 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Hayashi, Keiko
Yoshida, Tomofumi
Hayano-Saito, Yuriko
Detection of white head symptoms of panicle blast caused by Pyricularia oryzae using cut-flower dye
title Detection of white head symptoms of panicle blast caused by Pyricularia oryzae using cut-flower dye
title_full Detection of white head symptoms of panicle blast caused by Pyricularia oryzae using cut-flower dye
title_fullStr Detection of white head symptoms of panicle blast caused by Pyricularia oryzae using cut-flower dye
title_full_unstemmed Detection of white head symptoms of panicle blast caused by Pyricularia oryzae using cut-flower dye
title_short Detection of white head symptoms of panicle blast caused by Pyricularia oryzae using cut-flower dye
title_sort detection of white head symptoms of panicle blast caused by pyricularia oryzae using cut-flower dye
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6931245/
https://www.ncbi.nlm.nih.gov/pubmed/31889983
http://dx.doi.org/10.1186/s13007-019-0548-z
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