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In vivo and in vitro knockout system labelled using fluorescent protein via microhomology-mediated end joining
Gene knockout is important for understanding gene function and genetic disorders. The CRISPR/Cas9 system has great potential to achieve this purpose. However, we cannot distinguish visually whether a gene is knocked out and in how many cells it is knocked out among a population of cells. Here, we de...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Life Science Alliance LLC
2019
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6932181/ https://www.ncbi.nlm.nih.gov/pubmed/31874862 http://dx.doi.org/10.26508/lsa.201900528 |