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Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization

Poly (lactic acid) (PLA) has been increasingly used in cutaneous tissue engineering due to its low cost, ease of handling, biodegradability, and biocompatibility, as well as its ability to form composites. However, these polymers possess a structure with nanoporous that mimic the cellular environmen...

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Autores principales: Marsi, Teresa C. O., Ricci, Ritchelli, Toniato, Tatiane V., Vasconcellos, Luana M. R., Elias, Conceição de Maria Vaz, Silva, Andre D. R., Furtado, Andre S. A., Magalhães, Leila S. S. M., Silva-Filho, Edson C., Marciano, Fernanda R., Zille, Andrea, Webster, Thomas J., Lobo, Anderson O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6932955/
https://www.ncbi.nlm.nih.gov/pubmed/31921824
http://dx.doi.org/10.3389/fbioe.2019.00421
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author Marsi, Teresa C. O.
Ricci, Ritchelli
Toniato, Tatiane V.
Vasconcellos, Luana M. R.
Elias, Conceição de Maria Vaz
Silva, Andre D. R.
Furtado, Andre S. A.
Magalhães, Leila S. S. M.
Silva-Filho, Edson C.
Marciano, Fernanda R.
Zille, Andrea
Webster, Thomas J.
Lobo, Anderson O.
author_facet Marsi, Teresa C. O.
Ricci, Ritchelli
Toniato, Tatiane V.
Vasconcellos, Luana M. R.
Elias, Conceição de Maria Vaz
Silva, Andre D. R.
Furtado, Andre S. A.
Magalhães, Leila S. S. M.
Silva-Filho, Edson C.
Marciano, Fernanda R.
Zille, Andrea
Webster, Thomas J.
Lobo, Anderson O.
author_sort Marsi, Teresa C. O.
collection PubMed
description Poly (lactic acid) (PLA) has been increasingly used in cutaneous tissue engineering due to its low cost, ease of handling, biodegradability, and biocompatibility, as well as its ability to form composites. However, these polymers possess a structure with nanoporous that mimic the cellular environment. In this study, nanocomposites are prepared using PLA and titanium dioxide (TiO(2)) (10 and 35%—w/w) nanoparticles that also function as an active anti-scarring agent. The nanocomposites were prepared using an electrospinning technique. Three different solutions were prepared as follows: PLA, 10% PLA/TiO(2), and 35% PLA/TiO(2) (w/w%). Electrospun PLA and PLA/TiO(2) nanocomposites were characterized morphologically, structurally, and chemically using electron scanning microscopy, transmission electron microscopy, goniometry, and X-ray diffraction. L929 fibroblast cells were used for in vitro tests. The cytotoxic effect was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Versicam (VCAN), biglicam (BIG), interleukin-6 (IL6), interleukin-10 (IL-10), and type-1 collagen (COL1A1) genes were evaluated by RT-qPCR. In vivo tests using Wistar rats were conducted for up to 15 days. Nanofibrous fibers were obtained for all groups that did not contain residual solvents. No cytotoxic effects were observed for up to 168 h. The genes expressed showed the highest values of versican and collagen-1 (p < 0.05) for PLA/TiO(2) nanocomposite scaffolds when compared to the control group (cells). Histological images showed that PLA at 10 and 35% w/w led to a discrete inflammatory infiltration and expression of many newly formed vessels, indicating increased metabolic activity of this tissue. To summarize, this study supported the potential of PLA/TiO(2) nanocomposites ability to reduce cutaneous scarring in scaffolds.
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spelling pubmed-69329552020-01-09 Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization Marsi, Teresa C. O. Ricci, Ritchelli Toniato, Tatiane V. Vasconcellos, Luana M. R. Elias, Conceição de Maria Vaz Silva, Andre D. R. Furtado, Andre S. A. Magalhães, Leila S. S. M. Silva-Filho, Edson C. Marciano, Fernanda R. Zille, Andrea Webster, Thomas J. Lobo, Anderson O. Front Bioeng Biotechnol Bioengineering and Biotechnology Poly (lactic acid) (PLA) has been increasingly used in cutaneous tissue engineering due to its low cost, ease of handling, biodegradability, and biocompatibility, as well as its ability to form composites. However, these polymers possess a structure with nanoporous that mimic the cellular environment. In this study, nanocomposites are prepared using PLA and titanium dioxide (TiO(2)) (10 and 35%—w/w) nanoparticles that also function as an active anti-scarring agent. The nanocomposites were prepared using an electrospinning technique. Three different solutions were prepared as follows: PLA, 10% PLA/TiO(2), and 35% PLA/TiO(2) (w/w%). Electrospun PLA and PLA/TiO(2) nanocomposites were characterized morphologically, structurally, and chemically using electron scanning microscopy, transmission electron microscopy, goniometry, and X-ray diffraction. L929 fibroblast cells were used for in vitro tests. The cytotoxic effect was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Versicam (VCAN), biglicam (BIG), interleukin-6 (IL6), interleukin-10 (IL-10), and type-1 collagen (COL1A1) genes were evaluated by RT-qPCR. In vivo tests using Wistar rats were conducted for up to 15 days. Nanofibrous fibers were obtained for all groups that did not contain residual solvents. No cytotoxic effects were observed for up to 168 h. The genes expressed showed the highest values of versican and collagen-1 (p < 0.05) for PLA/TiO(2) nanocomposite scaffolds when compared to the control group (cells). Histological images showed that PLA at 10 and 35% w/w led to a discrete inflammatory infiltration and expression of many newly formed vessels, indicating increased metabolic activity of this tissue. To summarize, this study supported the potential of PLA/TiO(2) nanocomposites ability to reduce cutaneous scarring in scaffolds. Frontiers Media S.A. 2019-12-20 /pmc/articles/PMC6932955/ /pubmed/31921824 http://dx.doi.org/10.3389/fbioe.2019.00421 Text en Copyright © 2019 Marsi, Ricci, Toniato, Vasconcellos, Elias, Silva, Furtado, Magalhães, Silva-Filho, Marciano, Zille, Webster and Lobo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Marsi, Teresa C. O.
Ricci, Ritchelli
Toniato, Tatiane V.
Vasconcellos, Luana M. R.
Elias, Conceição de Maria Vaz
Silva, Andre D. R.
Furtado, Andre S. A.
Magalhães, Leila S. S. M.
Silva-Filho, Edson C.
Marciano, Fernanda R.
Zille, Andrea
Webster, Thomas J.
Lobo, Anderson O.
Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization
title Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization
title_full Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization
title_fullStr Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization
title_full_unstemmed Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization
title_short Electrospun Nanofibrous Poly (Lactic Acid)/Titanium Dioxide Nanocomposite Membranes for Cutaneous Scar Minimization
title_sort electrospun nanofibrous poly (lactic acid)/titanium dioxide nanocomposite membranes for cutaneous scar minimization
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6932955/
https://www.ncbi.nlm.nih.gov/pubmed/31921824
http://dx.doi.org/10.3389/fbioe.2019.00421
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