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Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development

Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind. Recent progress in the area of recombinant DNA technol...

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Autores principales: Tripathi, Nagesh K., Shrivastava, Ambuj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6932962/
https://www.ncbi.nlm.nih.gov/pubmed/31921823
http://dx.doi.org/10.3389/fbioe.2019.00420
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author Tripathi, Nagesh K.
Shrivastava, Ambuj
author_facet Tripathi, Nagesh K.
Shrivastava, Ambuj
author_sort Tripathi, Nagesh K.
collection PubMed
description Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind. Recent progress in the area of recombinant DNA technologies has paved the way to producing recombinant proteins that can be used as therapeutics, vaccines, and diagnostic reagents. Recombinant proteins for these applications are mainly produced using prokaryotic and eukaryotic expression host systems such as mammalian cells, bacteria, yeast, insect cells, and transgenic plants at laboratory scale as well as in large-scale settings. The development of efficient bioprocessing strategies is crucial for industrial production of recombinant proteins of therapeutic and prophylactic importance. Recently, advances have been made in the various areas of bioprocessing and are being utilized to develop effective processes for producing recombinant proteins. These include the use of high-throughput devices for effective bioprocess optimization and of disposable systems, continuous upstream processing, continuous chromatography, integrated continuous bioprocessing, Quality by Design, and process analytical technologies to achieve quality product with higher yield. This review summarizes recent developments in the bioprocessing of recombinant proteins, including in various expression systems, bioprocess development, and the upstream and downstream processing of recombinant proteins.
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spelling pubmed-69329622020-01-09 Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development Tripathi, Nagesh K. Shrivastava, Ambuj Front Bioeng Biotechnol Bioengineering and Biotechnology Infectious diseases, along with cancers, are among the main causes of death among humans worldwide. The production of therapeutic proteins for treating diseases at large scale for millions of individuals is one of the essential needs of mankind. Recent progress in the area of recombinant DNA technologies has paved the way to producing recombinant proteins that can be used as therapeutics, vaccines, and diagnostic reagents. Recombinant proteins for these applications are mainly produced using prokaryotic and eukaryotic expression host systems such as mammalian cells, bacteria, yeast, insect cells, and transgenic plants at laboratory scale as well as in large-scale settings. The development of efficient bioprocessing strategies is crucial for industrial production of recombinant proteins of therapeutic and prophylactic importance. Recently, advances have been made in the various areas of bioprocessing and are being utilized to develop effective processes for producing recombinant proteins. These include the use of high-throughput devices for effective bioprocess optimization and of disposable systems, continuous upstream processing, continuous chromatography, integrated continuous bioprocessing, Quality by Design, and process analytical technologies to achieve quality product with higher yield. This review summarizes recent developments in the bioprocessing of recombinant proteins, including in various expression systems, bioprocess development, and the upstream and downstream processing of recombinant proteins. Frontiers Media S.A. 2019-12-20 /pmc/articles/PMC6932962/ /pubmed/31921823 http://dx.doi.org/10.3389/fbioe.2019.00420 Text en Copyright © 2019 Tripathi and Shrivastava. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Tripathi, Nagesh K.
Shrivastava, Ambuj
Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development
title Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development
title_full Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development
title_fullStr Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development
title_full_unstemmed Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development
title_short Recent Developments in Bioprocessing of Recombinant Proteins: Expression Hosts and Process Development
title_sort recent developments in bioprocessing of recombinant proteins: expression hosts and process development
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6932962/
https://www.ncbi.nlm.nih.gov/pubmed/31921823
http://dx.doi.org/10.3389/fbioe.2019.00420
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