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Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion

The data presented in this article are related to the research article entitled as “Targeted deletion of the BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta-thalassemia disease " [1]. BCL11A is a master regulator of γ-globin gen...

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Autores principales: Khosravi, Mohammad Ali, Abbasalipour, Maryam, Concordet, Jean-Paul, Berg, Johannes vom, Zeinali, Sirous, Arashkia, Arash, Buch, Thorsten, Karimipoor, Morteza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933148/
https://www.ncbi.nlm.nih.gov/pubmed/31890812
http://dx.doi.org/10.1016/j.dib.2019.104974
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author Khosravi, Mohammad Ali
Abbasalipour, Maryam
Concordet, Jean-Paul
Berg, Johannes vom
Zeinali, Sirous
Arashkia, Arash
Buch, Thorsten
Karimipoor, Morteza
author_facet Khosravi, Mohammad Ali
Abbasalipour, Maryam
Concordet, Jean-Paul
Berg, Johannes vom
Zeinali, Sirous
Arashkia, Arash
Buch, Thorsten
Karimipoor, Morteza
author_sort Khosravi, Mohammad Ali
collection PubMed
description The data presented in this article are related to the research article entitled as “Targeted deletion of the BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta-thalassemia disease " [1]. BCL11A is a master regulator of γ-globin gene silencing, and suppresses fetal hemoglobin expression by association with other γ-globin suppressors, and also interacts with human beta-globin locus control region as well as intergenic region between the Aγ and δ-globin genes to reconfigure beta-globin cluster. Thus, HbF reactivation has been proposed to be an approach for the treatment of β-thalassemia through knockout of BCL11A. Accordingly, an erythroid enhancer sequence was identified that, when inactivated, led to repression of BCL11A and induction of γ-globin in the erythroid lineage [2–7]. This article describes data that obtained from BCL11A gene enhancer modification in KU812 and KG-1 cell lines using the CRISPR-Cas9 genome editing system in order to reactivate γ-globin gene expression.
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spelling pubmed-69331482019-12-30 Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion Khosravi, Mohammad Ali Abbasalipour, Maryam Concordet, Jean-Paul Berg, Johannes vom Zeinali, Sirous Arashkia, Arash Buch, Thorsten Karimipoor, Morteza Data Brief Biochemistry, Genetics and Molecular Biology The data presented in this article are related to the research article entitled as “Targeted deletion of the BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta-thalassemia disease " [1]. BCL11A is a master regulator of γ-globin gene silencing, and suppresses fetal hemoglobin expression by association with other γ-globin suppressors, and also interacts with human beta-globin locus control region as well as intergenic region between the Aγ and δ-globin genes to reconfigure beta-globin cluster. Thus, HbF reactivation has been proposed to be an approach for the treatment of β-thalassemia through knockout of BCL11A. Accordingly, an erythroid enhancer sequence was identified that, when inactivated, led to repression of BCL11A and induction of γ-globin in the erythroid lineage [2–7]. This article describes data that obtained from BCL11A gene enhancer modification in KU812 and KG-1 cell lines using the CRISPR-Cas9 genome editing system in order to reactivate γ-globin gene expression. Elsevier 2019-12-11 /pmc/articles/PMC6933148/ /pubmed/31890812 http://dx.doi.org/10.1016/j.dib.2019.104974 Text en © 2019 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Biochemistry, Genetics and Molecular Biology
Khosravi, Mohammad Ali
Abbasalipour, Maryam
Concordet, Jean-Paul
Berg, Johannes vom
Zeinali, Sirous
Arashkia, Arash
Buch, Thorsten
Karimipoor, Morteza
Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion
title Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion
title_full Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion
title_fullStr Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion
title_full_unstemmed Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion
title_short Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion
title_sort expression analysis data of bcl11a and γ-globin genes in ku812 and kg-1 cell lines after crispr/cas9-mediated bcl11a enhancer deletion
topic Biochemistry, Genetics and Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933148/
https://www.ncbi.nlm.nih.gov/pubmed/31890812
http://dx.doi.org/10.1016/j.dib.2019.104974
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