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Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion
The data presented in this article are related to the research article entitled as “Targeted deletion of the BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta-thalassemia disease " [1]. BCL11A is a master regulator of γ-globin gen...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933148/ https://www.ncbi.nlm.nih.gov/pubmed/31890812 http://dx.doi.org/10.1016/j.dib.2019.104974 |
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author | Khosravi, Mohammad Ali Abbasalipour, Maryam Concordet, Jean-Paul Berg, Johannes vom Zeinali, Sirous Arashkia, Arash Buch, Thorsten Karimipoor, Morteza |
author_facet | Khosravi, Mohammad Ali Abbasalipour, Maryam Concordet, Jean-Paul Berg, Johannes vom Zeinali, Sirous Arashkia, Arash Buch, Thorsten Karimipoor, Morteza |
author_sort | Khosravi, Mohammad Ali |
collection | PubMed |
description | The data presented in this article are related to the research article entitled as “Targeted deletion of the BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta-thalassemia disease " [1]. BCL11A is a master regulator of γ-globin gene silencing, and suppresses fetal hemoglobin expression by association with other γ-globin suppressors, and also interacts with human beta-globin locus control region as well as intergenic region between the Aγ and δ-globin genes to reconfigure beta-globin cluster. Thus, HbF reactivation has been proposed to be an approach for the treatment of β-thalassemia through knockout of BCL11A. Accordingly, an erythroid enhancer sequence was identified that, when inactivated, led to repression of BCL11A and induction of γ-globin in the erythroid lineage [2–7]. This article describes data that obtained from BCL11A gene enhancer modification in KU812 and KG-1 cell lines using the CRISPR-Cas9 genome editing system in order to reactivate γ-globin gene expression. |
format | Online Article Text |
id | pubmed-6933148 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-69331482019-12-30 Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion Khosravi, Mohammad Ali Abbasalipour, Maryam Concordet, Jean-Paul Berg, Johannes vom Zeinali, Sirous Arashkia, Arash Buch, Thorsten Karimipoor, Morteza Data Brief Biochemistry, Genetics and Molecular Biology The data presented in this article are related to the research article entitled as “Targeted deletion of the BCL11A gene by CRISPR-Cas9 system for fetal hemoglobin reactivation: A promising approach for gene therapy of beta-thalassemia disease " [1]. BCL11A is a master regulator of γ-globin gene silencing, and suppresses fetal hemoglobin expression by association with other γ-globin suppressors, and also interacts with human beta-globin locus control region as well as intergenic region between the Aγ and δ-globin genes to reconfigure beta-globin cluster. Thus, HbF reactivation has been proposed to be an approach for the treatment of β-thalassemia through knockout of BCL11A. Accordingly, an erythroid enhancer sequence was identified that, when inactivated, led to repression of BCL11A and induction of γ-globin in the erythroid lineage [2–7]. This article describes data that obtained from BCL11A gene enhancer modification in KU812 and KG-1 cell lines using the CRISPR-Cas9 genome editing system in order to reactivate γ-globin gene expression. Elsevier 2019-12-11 /pmc/articles/PMC6933148/ /pubmed/31890812 http://dx.doi.org/10.1016/j.dib.2019.104974 Text en © 2019 Published by Elsevier Inc. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Biochemistry, Genetics and Molecular Biology Khosravi, Mohammad Ali Abbasalipour, Maryam Concordet, Jean-Paul Berg, Johannes vom Zeinali, Sirous Arashkia, Arash Buch, Thorsten Karimipoor, Morteza Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion |
title | Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion |
title_full | Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion |
title_fullStr | Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion |
title_full_unstemmed | Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion |
title_short | Expression analysis data of BCL11A and γ-globin genes in KU812 and KG-1 cell lines after CRISPR/Cas9-mediated BCL11A enhancer deletion |
title_sort | expression analysis data of bcl11a and γ-globin genes in ku812 and kg-1 cell lines after crispr/cas9-mediated bcl11a enhancer deletion |
topic | Biochemistry, Genetics and Molecular Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933148/ https://www.ncbi.nlm.nih.gov/pubmed/31890812 http://dx.doi.org/10.1016/j.dib.2019.104974 |
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