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Genotoxicity and repair capability of Mus musculus DNA following the oral exposure to Tramadol

Tramadol is an analgesic and psychoactive drug that acts primarily upon the central nervous system where it alters brain function, resulting in temporary changes in perception, mood, consciousness and behavior. The aim of present study was to analyze the genotoxicity and repair capability of DNA aft...

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Autores principales: Ali, Tayyaba, Rafiq, Maleeha, Samee Mubarik, Muhammad, Zahoor, Kashif, Asad, Farkhanda, Yaqoob, Sajid, Ahmad, Shahzad, Qamar, Samina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933236/
https://www.ncbi.nlm.nih.gov/pubmed/31889811
http://dx.doi.org/10.1016/j.sjbs.2019.03.008
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author Ali, Tayyaba
Rafiq, Maleeha
Samee Mubarik, Muhammad
Zahoor, Kashif
Asad, Farkhanda
Yaqoob, Sajid
Ahmad, Shahzad
Qamar, Samina
author_facet Ali, Tayyaba
Rafiq, Maleeha
Samee Mubarik, Muhammad
Zahoor, Kashif
Asad, Farkhanda
Yaqoob, Sajid
Ahmad, Shahzad
Qamar, Samina
author_sort Ali, Tayyaba
collection PubMed
description Tramadol is an analgesic and psychoactive drug that acts primarily upon the central nervous system where it alters brain function, resulting in temporary changes in perception, mood, consciousness and behavior. The aim of present study was to analyze the genotoxicity and repair capability of DNA after Tramadol exposure in albino mice (Mus musculus). For this purpose, forty mice were divided equally into four groups as; a control group (without drug) and three treatment groups that were treated with three doses of Tramadol as minimum dose group, Intermediate dose group and maximum dose group, corresponding to 25 mg/kg, 50 mg/kg and 75 mg/kg of body weight respectively. The dose was given orally for 15 days. After 15 days peripheral blood was drawn from half mice of each group and subjected to comet assay. While the remaining half mice were given a recovery period of 15 days and same procedure was used for blood collection and comet assay. Significant difference in various comet parameters was observed among control and exposed groups. Maximum damage was observed at highest concentration 75 mg/kg of Tramadol and minimum damage was observed at dose 25 mg/kg of Tramadol, while results of repaired mice group showed that repair capability of Tramadol was minor and recovery of Tramadol required a lot of time. It can be concluded that Tramadol cause genotoxicity that is dose dependent and has low repair capability.
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spelling pubmed-69332362019-12-30 Genotoxicity and repair capability of Mus musculus DNA following the oral exposure to Tramadol Ali, Tayyaba Rafiq, Maleeha Samee Mubarik, Muhammad Zahoor, Kashif Asad, Farkhanda Yaqoob, Sajid Ahmad, Shahzad Qamar, Samina Saudi J Biol Sci Article Tramadol is an analgesic and psychoactive drug that acts primarily upon the central nervous system where it alters brain function, resulting in temporary changes in perception, mood, consciousness and behavior. The aim of present study was to analyze the genotoxicity and repair capability of DNA after Tramadol exposure in albino mice (Mus musculus). For this purpose, forty mice were divided equally into four groups as; a control group (without drug) and three treatment groups that were treated with three doses of Tramadol as minimum dose group, Intermediate dose group and maximum dose group, corresponding to 25 mg/kg, 50 mg/kg and 75 mg/kg of body weight respectively. The dose was given orally for 15 days. After 15 days peripheral blood was drawn from half mice of each group and subjected to comet assay. While the remaining half mice were given a recovery period of 15 days and same procedure was used for blood collection and comet assay. Significant difference in various comet parameters was observed among control and exposed groups. Maximum damage was observed at highest concentration 75 mg/kg of Tramadol and minimum damage was observed at dose 25 mg/kg of Tramadol, while results of repaired mice group showed that repair capability of Tramadol was minor and recovery of Tramadol required a lot of time. It can be concluded that Tramadol cause genotoxicity that is dose dependent and has low repair capability. Elsevier 2020-01 2019-03-26 /pmc/articles/PMC6933236/ /pubmed/31889811 http://dx.doi.org/10.1016/j.sjbs.2019.03.008 Text en © 2019 Production and hosting by Elsevier B.V. on behalf of King Saud University. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Ali, Tayyaba
Rafiq, Maleeha
Samee Mubarik, Muhammad
Zahoor, Kashif
Asad, Farkhanda
Yaqoob, Sajid
Ahmad, Shahzad
Qamar, Samina
Genotoxicity and repair capability of Mus musculus DNA following the oral exposure to Tramadol
title Genotoxicity and repair capability of Mus musculus DNA following the oral exposure to Tramadol
title_full Genotoxicity and repair capability of Mus musculus DNA following the oral exposure to Tramadol
title_fullStr Genotoxicity and repair capability of Mus musculus DNA following the oral exposure to Tramadol
title_full_unstemmed Genotoxicity and repair capability of Mus musculus DNA following the oral exposure to Tramadol
title_short Genotoxicity and repair capability of Mus musculus DNA following the oral exposure to Tramadol
title_sort genotoxicity and repair capability of mus musculus dna following the oral exposure to tramadol
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933236/
https://www.ncbi.nlm.nih.gov/pubmed/31889811
http://dx.doi.org/10.1016/j.sjbs.2019.03.008
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