Biological role of mycorrhizal fungi on the assimilation and transportation of carbon and nitrogen to Anacamptis palustris and Anacamptis laxiflor

Fungal is a physiological trail and its understanding in the assimilation with the transfer of carbon (C) cum nitrogen (N) or (C/N) to orchid-seedlings have not been determined. Labelled stable isotopes (13)C and (15)N were used to plan the flow of C and N between orchid plants and mycorrhizal connotations in-terms of bulk transfer for C/N. This study attends to comprehend the mechanism, supporting mycorrhizal fungi which influences on orchid-seedling growth. Determined integration and transfer of C/N from amino acids (AA), ammonium nitrate (NH(4)NO(3)) and sugar for orchid-plant may lead to understand these mechanisms. This current study tries to estimate the importance of organic compounds as a source for C/N over the inorganic-NH(4)NO(3). Generally, after begging of germination and when it is found to be associated to the nutrient resource, organic compound enhance the biomass accumulation of two orchid species. AA significantly increase the mass of (13)C assimilated by two species. With amino acids the concentration of (13)C in two species was greater than with NH(4)NO(3) and sugar. At another phase, amount of (15)N content shoots was a higher value in Anacamptis laxiflora shoots assimilated substantially additional of (15)N with NH(4)NO(3) plus sugar compared with ammonium nitrate only. This study showed that two terrestrial orchids species are reliant on organic compounds as a source of carbon and nitrogen more than inorganic compounds.