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Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia

A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992–2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009–2011, 2015...

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Autores principales: Matović, Kazimir, Vidanović, Dejan, Manić, Marija, Stojiljković, Marko, Radojičić, Sonja, Debeljak, Zoran, Šekler, Milanko, Ćirić, Jelena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933281/
https://www.ncbi.nlm.nih.gov/pubmed/31889877
http://dx.doi.org/10.1016/j.sjbs.2019.11.012
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author Matović, Kazimir
Vidanović, Dejan
Manić, Marija
Stojiljković, Marko
Radojičić, Sonja
Debeljak, Zoran
Šekler, Milanko
Ćirić, Jelena
author_facet Matović, Kazimir
Vidanović, Dejan
Manić, Marija
Stojiljković, Marko
Radojičić, Sonja
Debeljak, Zoran
Šekler, Milanko
Ćirić, Jelena
author_sort Matović, Kazimir
collection PubMed
description A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992–2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009–2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.
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spelling pubmed-69332812019-12-30 Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia Matović, Kazimir Vidanović, Dejan Manić, Marija Stojiljković, Marko Radojičić, Sonja Debeljak, Zoran Šekler, Milanko Ćirić, Jelena Saudi J Biol Sci Article A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992–2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009–2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection. Elsevier 2020-01 2019-11-21 /pmc/articles/PMC6933281/ /pubmed/31889877 http://dx.doi.org/10.1016/j.sjbs.2019.11.012 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Matović, Kazimir
Vidanović, Dejan
Manić, Marija
Stojiljković, Marko
Radojičić, Sonja
Debeljak, Zoran
Šekler, Milanko
Ćirić, Jelena
Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
title Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
title_full Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
title_fullStr Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
title_full_unstemmed Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
title_short Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
title_sort twenty-five-year study of nosema spp. in honey bees (apis mellifera) in serbia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933281/
https://www.ncbi.nlm.nih.gov/pubmed/31889877
http://dx.doi.org/10.1016/j.sjbs.2019.11.012
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