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Developmental Changes of Glutamate and GABA Receptor Densities in Wistar Rats
Neurotransmitters and their receptors are key molecules of signal transduction and subject to various changes during pre- and postnatal development. Previous studies addressed ontogeny at the level of neurotransmitters and expression of neurotransmitter receptor subunits. However, developmental chan...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933313/ https://www.ncbi.nlm.nih.gov/pubmed/31920569 http://dx.doi.org/10.3389/fnana.2019.00100 |
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author | Behuet, Sabrina Cremer, Jennifer Nadine Cremer, Markus Palomero-Gallagher, Nicola Zilles, Karl Amunts, Katrin |
author_facet | Behuet, Sabrina Cremer, Jennifer Nadine Cremer, Markus Palomero-Gallagher, Nicola Zilles, Karl Amunts, Katrin |
author_sort | Behuet, Sabrina |
collection | PubMed |
description | Neurotransmitters and their receptors are key molecules of signal transduction and subject to various changes during pre- and postnatal development. Previous studies addressed ontogeny at the level of neurotransmitters and expression of neurotransmitter receptor subunits. However, developmental changes in receptor densities to this day are not well understood. Here, we analyzed developmental changes in excitatory glutamate and inhibitory γ-aminobutyric acid (GABA) receptors in adjacent sections of the rat brain by means of quantitative in vitro receptor autoradiography. Receptor densities of the ionotropic glutamatergic receptors α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), kainate and N-methyl-D-aspartate (NMDA) as well as of the ionotropic GABA(A) and metabotropic GABA(B) receptors were investigated using specific high-affinity ligands. For each receptor binding site, significant density differences were demonstrated in the investigated regions of interest [olfactory bulb, striatum, hippocampus, and cerebellum] and developmental stages [postnatal day (P) 0, 10, 20, 30 and 90]. In particular, we showed that the glutamatergic and GABAergic receptor densities were already present between P0 and P10 in all regions of interest, which may indicate the early relevance of these receptors for brain development. A transient increase of glutamatergic receptor densities in the hippocampus was found, indicating their possible involvement in synaptic plasticity. We demonstrated a decline of NMDA receptor densities in the striatum and hippocampus from P30 to P90, which could be due to synapse elimination, a process that redefines neuronal networks in postnatal brains. Furthermore, the highest increase in GABA(A) receptor densities from P10 to P20 coincides with the developmental shift from excitatory to inhibitory GABA transmission. Moreover, the increase from P10 to P20 in GABA(A) receptor densities in the cerebellum corresponds to a point in time when functional GABAergic synapses are formed. Taken together, the present data reveal differential changes in glutamate and GABA receptor densities during postnatal rat brain development, which may contribute to their specific functions during ontogenesis, thus providing a deeper understanding of brain ontogenesis and receptor function. |
format | Online Article Text |
id | pubmed-6933313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69333132020-01-09 Developmental Changes of Glutamate and GABA Receptor Densities in Wistar Rats Behuet, Sabrina Cremer, Jennifer Nadine Cremer, Markus Palomero-Gallagher, Nicola Zilles, Karl Amunts, Katrin Front Neuroanat Neuroscience Neurotransmitters and their receptors are key molecules of signal transduction and subject to various changes during pre- and postnatal development. Previous studies addressed ontogeny at the level of neurotransmitters and expression of neurotransmitter receptor subunits. However, developmental changes in receptor densities to this day are not well understood. Here, we analyzed developmental changes in excitatory glutamate and inhibitory γ-aminobutyric acid (GABA) receptors in adjacent sections of the rat brain by means of quantitative in vitro receptor autoradiography. Receptor densities of the ionotropic glutamatergic receptors α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), kainate and N-methyl-D-aspartate (NMDA) as well as of the ionotropic GABA(A) and metabotropic GABA(B) receptors were investigated using specific high-affinity ligands. For each receptor binding site, significant density differences were demonstrated in the investigated regions of interest [olfactory bulb, striatum, hippocampus, and cerebellum] and developmental stages [postnatal day (P) 0, 10, 20, 30 and 90]. In particular, we showed that the glutamatergic and GABAergic receptor densities were already present between P0 and P10 in all regions of interest, which may indicate the early relevance of these receptors for brain development. A transient increase of glutamatergic receptor densities in the hippocampus was found, indicating their possible involvement in synaptic plasticity. We demonstrated a decline of NMDA receptor densities in the striatum and hippocampus from P30 to P90, which could be due to synapse elimination, a process that redefines neuronal networks in postnatal brains. Furthermore, the highest increase in GABA(A) receptor densities from P10 to P20 coincides with the developmental shift from excitatory to inhibitory GABA transmission. Moreover, the increase from P10 to P20 in GABA(A) receptor densities in the cerebellum corresponds to a point in time when functional GABAergic synapses are formed. Taken together, the present data reveal differential changes in glutamate and GABA receptor densities during postnatal rat brain development, which may contribute to their specific functions during ontogenesis, thus providing a deeper understanding of brain ontogenesis and receptor function. Frontiers Media S.A. 2019-12-20 /pmc/articles/PMC6933313/ /pubmed/31920569 http://dx.doi.org/10.3389/fnana.2019.00100 Text en Copyright © 2019 Behuet, Cremer, Cremer, Palomero-Gallagher, Zilles and Amunts. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Behuet, Sabrina Cremer, Jennifer Nadine Cremer, Markus Palomero-Gallagher, Nicola Zilles, Karl Amunts, Katrin Developmental Changes of Glutamate and GABA Receptor Densities in Wistar Rats |
title | Developmental Changes of Glutamate and GABA Receptor Densities in Wistar Rats |
title_full | Developmental Changes of Glutamate and GABA Receptor Densities in Wistar Rats |
title_fullStr | Developmental Changes of Glutamate and GABA Receptor Densities in Wistar Rats |
title_full_unstemmed | Developmental Changes of Glutamate and GABA Receptor Densities in Wistar Rats |
title_short | Developmental Changes of Glutamate and GABA Receptor Densities in Wistar Rats |
title_sort | developmental changes of glutamate and gaba receptor densities in wistar rats |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933313/ https://www.ncbi.nlm.nih.gov/pubmed/31920569 http://dx.doi.org/10.3389/fnana.2019.00100 |
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