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Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke
Endogenous neurogenesis holds promise for brain repair and long‐term functional recovery after ischaemic stroke. However, the effects of exosomes from human urine‐derived stem cells (USC‐Exos) in neurogenesis remain unclear. This study aimed to investigate whether USC‐Exos enhanced neurogenesis and...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933407/ https://www.ncbi.nlm.nih.gov/pubmed/31667951 http://dx.doi.org/10.1111/jcmm.14774 |
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author | Ling, Xiaozheng Zhang, Guowei Xia, Yuguo Zhu, Qingwei Zhang, Juntao Li, Qing Niu, Xin Hu, Guowen Yang, Yunlong Wang, Yang Deng, Zhifeng |
author_facet | Ling, Xiaozheng Zhang, Guowei Xia, Yuguo Zhu, Qingwei Zhang, Juntao Li, Qing Niu, Xin Hu, Guowen Yang, Yunlong Wang, Yang Deng, Zhifeng |
author_sort | Ling, Xiaozheng |
collection | PubMed |
description | Endogenous neurogenesis holds promise for brain repair and long‐term functional recovery after ischaemic stroke. However, the effects of exosomes from human urine‐derived stem cells (USC‐Exos) in neurogenesis remain unclear. This study aimed to investigate whether USC‐Exos enhanced neurogenesis and promoted functional recovery in brain ischaemia. By using an experimental stroke rat model, we found that intravenous injection of USC‐Exos enhanced neurogenesis and alleviated neurological deficits in post‐ischaemic stroke rats. We used neural stem cells (NSCs) subjected to oxygen‐glucose deprivation/reoxygenation (OGD/R) as an in vitro model of ischaemic stroke. The in vitro results suggested that USC‐Exos promoted both proliferation and neuronal differentiation of NSCs after OGD/R. Notably, a further mechanism study revealed that the pro‐neurogenesis effects of USC‐Exos may be partially attributed to histone deacetylase 6 (HDAC6) inhibition via the transfer of exosomal microRNA‐26a (miR‐26a). Taken together, this study indicates that USC‐Exos can be used as a novel promising strategy for brain ischaemia, which highlights the application of USC‐Exos. |
format | Online Article Text |
id | pubmed-6933407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69334072020-01-01 Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke Ling, Xiaozheng Zhang, Guowei Xia, Yuguo Zhu, Qingwei Zhang, Juntao Li, Qing Niu, Xin Hu, Guowen Yang, Yunlong Wang, Yang Deng, Zhifeng J Cell Mol Med Original Articles Endogenous neurogenesis holds promise for brain repair and long‐term functional recovery after ischaemic stroke. However, the effects of exosomes from human urine‐derived stem cells (USC‐Exos) in neurogenesis remain unclear. This study aimed to investigate whether USC‐Exos enhanced neurogenesis and promoted functional recovery in brain ischaemia. By using an experimental stroke rat model, we found that intravenous injection of USC‐Exos enhanced neurogenesis and alleviated neurological deficits in post‐ischaemic stroke rats. We used neural stem cells (NSCs) subjected to oxygen‐glucose deprivation/reoxygenation (OGD/R) as an in vitro model of ischaemic stroke. The in vitro results suggested that USC‐Exos promoted both proliferation and neuronal differentiation of NSCs after OGD/R. Notably, a further mechanism study revealed that the pro‐neurogenesis effects of USC‐Exos may be partially attributed to histone deacetylase 6 (HDAC6) inhibition via the transfer of exosomal microRNA‐26a (miR‐26a). Taken together, this study indicates that USC‐Exos can be used as a novel promising strategy for brain ischaemia, which highlights the application of USC‐Exos. John Wiley and Sons Inc. 2019-10-31 2020-01 /pmc/articles/PMC6933407/ /pubmed/31667951 http://dx.doi.org/10.1111/jcmm.14774 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Ling, Xiaozheng Zhang, Guowei Xia, Yuguo Zhu, Qingwei Zhang, Juntao Li, Qing Niu, Xin Hu, Guowen Yang, Yunlong Wang, Yang Deng, Zhifeng Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke |
title | Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke |
title_full | Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke |
title_fullStr | Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke |
title_full_unstemmed | Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke |
title_short | Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke |
title_sort | exosomes from human urine‐derived stem cells enhanced neurogenesis via mir‐26a/hdac6 axis after ischaemic stroke |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933407/ https://www.ncbi.nlm.nih.gov/pubmed/31667951 http://dx.doi.org/10.1111/jcmm.14774 |
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