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Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke

Endogenous neurogenesis holds promise for brain repair and long‐term functional recovery after ischaemic stroke. However, the effects of exosomes from human urine‐derived stem cells (USC‐Exos) in neurogenesis remain unclear. This study aimed to investigate whether USC‐Exos enhanced neurogenesis and...

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Autores principales: Ling, Xiaozheng, Zhang, Guowei, Xia, Yuguo, Zhu, Qingwei, Zhang, Juntao, Li, Qing, Niu, Xin, Hu, Guowen, Yang, Yunlong, Wang, Yang, Deng, Zhifeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933407/
https://www.ncbi.nlm.nih.gov/pubmed/31667951
http://dx.doi.org/10.1111/jcmm.14774
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author Ling, Xiaozheng
Zhang, Guowei
Xia, Yuguo
Zhu, Qingwei
Zhang, Juntao
Li, Qing
Niu, Xin
Hu, Guowen
Yang, Yunlong
Wang, Yang
Deng, Zhifeng
author_facet Ling, Xiaozheng
Zhang, Guowei
Xia, Yuguo
Zhu, Qingwei
Zhang, Juntao
Li, Qing
Niu, Xin
Hu, Guowen
Yang, Yunlong
Wang, Yang
Deng, Zhifeng
author_sort Ling, Xiaozheng
collection PubMed
description Endogenous neurogenesis holds promise for brain repair and long‐term functional recovery after ischaemic stroke. However, the effects of exosomes from human urine‐derived stem cells (USC‐Exos) in neurogenesis remain unclear. This study aimed to investigate whether USC‐Exos enhanced neurogenesis and promoted functional recovery in brain ischaemia. By using an experimental stroke rat model, we found that intravenous injection of USC‐Exos enhanced neurogenesis and alleviated neurological deficits in post‐ischaemic stroke rats. We used neural stem cells (NSCs) subjected to oxygen‐glucose deprivation/reoxygenation (OGD/R) as an in vitro model of ischaemic stroke. The in vitro results suggested that USC‐Exos promoted both proliferation and neuronal differentiation of NSCs after OGD/R. Notably, a further mechanism study revealed that the pro‐neurogenesis effects of USC‐Exos may be partially attributed to histone deacetylase 6 (HDAC6) inhibition via the transfer of exosomal microRNA‐26a (miR‐26a). Taken together, this study indicates that USC‐Exos can be used as a novel promising strategy for brain ischaemia, which highlights the application of USC‐Exos.
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spelling pubmed-69334072020-01-01 Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke Ling, Xiaozheng Zhang, Guowei Xia, Yuguo Zhu, Qingwei Zhang, Juntao Li, Qing Niu, Xin Hu, Guowen Yang, Yunlong Wang, Yang Deng, Zhifeng J Cell Mol Med Original Articles Endogenous neurogenesis holds promise for brain repair and long‐term functional recovery after ischaemic stroke. However, the effects of exosomes from human urine‐derived stem cells (USC‐Exos) in neurogenesis remain unclear. This study aimed to investigate whether USC‐Exos enhanced neurogenesis and promoted functional recovery in brain ischaemia. By using an experimental stroke rat model, we found that intravenous injection of USC‐Exos enhanced neurogenesis and alleviated neurological deficits in post‐ischaemic stroke rats. We used neural stem cells (NSCs) subjected to oxygen‐glucose deprivation/reoxygenation (OGD/R) as an in vitro model of ischaemic stroke. The in vitro results suggested that USC‐Exos promoted both proliferation and neuronal differentiation of NSCs after OGD/R. Notably, a further mechanism study revealed that the pro‐neurogenesis effects of USC‐Exos may be partially attributed to histone deacetylase 6 (HDAC6) inhibition via the transfer of exosomal microRNA‐26a (miR‐26a). Taken together, this study indicates that USC‐Exos can be used as a novel promising strategy for brain ischaemia, which highlights the application of USC‐Exos. John Wiley and Sons Inc. 2019-10-31 2020-01 /pmc/articles/PMC6933407/ /pubmed/31667951 http://dx.doi.org/10.1111/jcmm.14774 Text en © 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Ling, Xiaozheng
Zhang, Guowei
Xia, Yuguo
Zhu, Qingwei
Zhang, Juntao
Li, Qing
Niu, Xin
Hu, Guowen
Yang, Yunlong
Wang, Yang
Deng, Zhifeng
Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke
title Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke
title_full Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke
title_fullStr Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke
title_full_unstemmed Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke
title_short Exosomes from human urine‐derived stem cells enhanced neurogenesis via miR‐26a/HDAC6 axis after ischaemic stroke
title_sort exosomes from human urine‐derived stem cells enhanced neurogenesis via mir‐26a/hdac6 axis after ischaemic stroke
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933407/
https://www.ncbi.nlm.nih.gov/pubmed/31667951
http://dx.doi.org/10.1111/jcmm.14774
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