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Numerical investigation of particle dispersion in the preprocessing stage for a static cell cultivation
In order to produce high-quality cells in a static culture, the initial placement of the cells is one of the most important factors. Dense distribution of the cells increases the risk of cell death. Thus, the cells need to be uniformly distributed during the preprocessing of a static culture. This p...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society for Regenerative Medicine
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933446/ https://www.ncbi.nlm.nih.gov/pubmed/31890770 http://dx.doi.org/10.1016/j.reth.2019.04.003 |
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author | Sekimoto, Atsushi Kanemaru, Yoshiki Okano, Yasunori Kanie, Kei Kato, Ryuji Kino-oka, Masahiro |
author_facet | Sekimoto, Atsushi Kanemaru, Yoshiki Okano, Yasunori Kanie, Kei Kato, Ryuji Kino-oka, Masahiro |
author_sort | Sekimoto, Atsushi |
collection | PubMed |
description | In order to produce high-quality cells in a static culture, the initial placement of the cells is one of the most important factors. Dense distribution of the cells increases the risk of cell death. Thus, the cells need to be uniformly distributed during the preprocessing of a static culture. This process depends on the operator's experience and has not been standardized. In this study, we have performed numerical simulations to investigate the efficiency of cell dispersion by using OpenFOAM. The numerical domain is a square-shaped dish. Two shaking methods, one-direction and multi-direction reciprocal shaking, were considered and calculations were conducted under five oscillation frequencies. The cell colony was assumed as a solid spherical particle. The initial particles were densely positioned at the center. The numerical result showed that the multi-direction reciprocal shaking was more effective to disperse the particles than the one-direction reciprocal shaking. In addition, at a low frequency, almost all particles sank to the bottom and hardly dispersed. These results indicate that strong fluctuations can lift particles from the bottom and that frequent changes in the flow direction make for more even distribution. |
format | Online Article Text |
id | pubmed-6933446 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Japanese Society for Regenerative Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-69334462019-12-30 Numerical investigation of particle dispersion in the preprocessing stage for a static cell cultivation Sekimoto, Atsushi Kanemaru, Yoshiki Okano, Yasunori Kanie, Kei Kato, Ryuji Kino-oka, Masahiro Regen Ther Article In order to produce high-quality cells in a static culture, the initial placement of the cells is one of the most important factors. Dense distribution of the cells increases the risk of cell death. Thus, the cells need to be uniformly distributed during the preprocessing of a static culture. This process depends on the operator's experience and has not been standardized. In this study, we have performed numerical simulations to investigate the efficiency of cell dispersion by using OpenFOAM. The numerical domain is a square-shaped dish. Two shaking methods, one-direction and multi-direction reciprocal shaking, were considered and calculations were conducted under five oscillation frequencies. The cell colony was assumed as a solid spherical particle. The initial particles were densely positioned at the center. The numerical result showed that the multi-direction reciprocal shaking was more effective to disperse the particles than the one-direction reciprocal shaking. In addition, at a low frequency, almost all particles sank to the bottom and hardly dispersed. These results indicate that strong fluctuations can lift particles from the bottom and that frequent changes in the flow direction make for more even distribution. Japanese Society for Regenerative Medicine 2019-04-25 /pmc/articles/PMC6933446/ /pubmed/31890770 http://dx.doi.org/10.1016/j.reth.2019.04.003 Text en © 2019 The Japanese Society for Regenerative Medicine. Production and hosting by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Sekimoto, Atsushi Kanemaru, Yoshiki Okano, Yasunori Kanie, Kei Kato, Ryuji Kino-oka, Masahiro Numerical investigation of particle dispersion in the preprocessing stage for a static cell cultivation |
title | Numerical investigation of particle dispersion in the preprocessing stage for a static cell cultivation |
title_full | Numerical investigation of particle dispersion in the preprocessing stage for a static cell cultivation |
title_fullStr | Numerical investigation of particle dispersion in the preprocessing stage for a static cell cultivation |
title_full_unstemmed | Numerical investigation of particle dispersion in the preprocessing stage for a static cell cultivation |
title_short | Numerical investigation of particle dispersion in the preprocessing stage for a static cell cultivation |
title_sort | numerical investigation of particle dispersion in the preprocessing stage for a static cell cultivation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6933446/ https://www.ncbi.nlm.nih.gov/pubmed/31890770 http://dx.doi.org/10.1016/j.reth.2019.04.003 |
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