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Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils

To develop biofertilizers for rice in Afghanistan, 98 plant growth-promoting rhizobacteria were isolated from rice plants and their morphological and physiological characteristics, such as indole-3-acetic acid production, acetylene reduction, phosphate and potassium solubilization, and siderophore p...

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Autores principales: Habibi, Safiullah, Djedidi, Salem, Ohkama-Ohtsu, Naoko, Sarhadi, Wakil Ahmad, Kojima, Katsuhiro, Rallos, Roland V., Ramirez, Maria Daniela Artigas, Yamaya, Hiroko, Sekimoto, Hitoshi, Yokoyama, Tadashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology (JSME)/Japanese Society of Soil Microbiology (JSSM)/Taiwan Society of Microbial Ecology (TSME)/Japanese Society of Plant Microbe Interactions (JSPMI)/Japanese Society for Extremophiles (JSE) 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934389/
https://www.ncbi.nlm.nih.gov/pubmed/31527341
http://dx.doi.org/10.1264/jsme2.ME18168
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author Habibi, Safiullah
Djedidi, Salem
Ohkama-Ohtsu, Naoko
Sarhadi, Wakil Ahmad
Kojima, Katsuhiro
Rallos, Roland V.
Ramirez, Maria Daniela Artigas
Yamaya, Hiroko
Sekimoto, Hitoshi
Yokoyama, Tadashi
author_facet Habibi, Safiullah
Djedidi, Salem
Ohkama-Ohtsu, Naoko
Sarhadi, Wakil Ahmad
Kojima, Katsuhiro
Rallos, Roland V.
Ramirez, Maria Daniela Artigas
Yamaya, Hiroko
Sekimoto, Hitoshi
Yokoyama, Tadashi
author_sort Habibi, Safiullah
collection PubMed
description To develop biofertilizers for rice in Afghanistan, 98 plant growth-promoting rhizobacteria were isolated from rice plants and their morphological and physiological characteristics, such as indole-3-acetic acid production, acetylene reduction, phosphate and potassium solubilization, and siderophore production, were evaluated. The genetic diversity of these bacteria was also analyzed based on 16S rRNA gene sequences. Of 98 bacteria, 89.7% produced IAA, 54.0% exhibited nitrogenase activity, and 40% showed phosphate solubilization and siderophore production. Some isolates assigned to Pseudomonas (brassicacearum, chengduensis, plecoglossicida, resinovorans, and straminea) formed a relationship with rice, and P. resinovorans and P. straminea showed nitrogen fixation. Rhizobium borbori and R. rosettiformans showed a relationship with rice plants and nitrogen fixation. Among the isolates examined, AF134 and AF137 belonging to Enterobacter ludwigii and P. putida produced large amounts of IAA (92.3 μg mL(−1)) and exhibited high nitrogenase activity (647.4 nmol C(2)H(4) h(−1)), respectively. In the plant growth test, more than 70% of the inoculated isolates showed significantly increased root and shoot dry weights. Highly diverse bacterial isolates showing promising rice growth-promoting traits were obtained from Afghanistan alkaline soils.
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spelling pubmed-69343892020-01-02 Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils Habibi, Safiullah Djedidi, Salem Ohkama-Ohtsu, Naoko Sarhadi, Wakil Ahmad Kojima, Katsuhiro Rallos, Roland V. Ramirez, Maria Daniela Artigas Yamaya, Hiroko Sekimoto, Hitoshi Yokoyama, Tadashi Microbes Environ Articles To develop biofertilizers for rice in Afghanistan, 98 plant growth-promoting rhizobacteria were isolated from rice plants and their morphological and physiological characteristics, such as indole-3-acetic acid production, acetylene reduction, phosphate and potassium solubilization, and siderophore production, were evaluated. The genetic diversity of these bacteria was also analyzed based on 16S rRNA gene sequences. Of 98 bacteria, 89.7% produced IAA, 54.0% exhibited nitrogenase activity, and 40% showed phosphate solubilization and siderophore production. Some isolates assigned to Pseudomonas (brassicacearum, chengduensis, plecoglossicida, resinovorans, and straminea) formed a relationship with rice, and P. resinovorans and P. straminea showed nitrogen fixation. Rhizobium borbori and R. rosettiformans showed a relationship with rice plants and nitrogen fixation. Among the isolates examined, AF134 and AF137 belonging to Enterobacter ludwigii and P. putida produced large amounts of IAA (92.3 μg mL(−1)) and exhibited high nitrogenase activity (647.4 nmol C(2)H(4) h(−1)), respectively. In the plant growth test, more than 70% of the inoculated isolates showed significantly increased root and shoot dry weights. Highly diverse bacterial isolates showing promising rice growth-promoting traits were obtained from Afghanistan alkaline soils. Japanese Society of Microbial Ecology (JSME)/Japanese Society of Soil Microbiology (JSSM)/Taiwan Society of Microbial Ecology (TSME)/Japanese Society of Plant Microbe Interactions (JSPMI)/Japanese Society for Extremophiles (JSE) 2019-12 2019-12-27 /pmc/articles/PMC6934389/ /pubmed/31527341 http://dx.doi.org/10.1264/jsme2.ME18168 Text en Copyright © 2019 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Habibi, Safiullah
Djedidi, Salem
Ohkama-Ohtsu, Naoko
Sarhadi, Wakil Ahmad
Kojima, Katsuhiro
Rallos, Roland V.
Ramirez, Maria Daniela Artigas
Yamaya, Hiroko
Sekimoto, Hitoshi
Yokoyama, Tadashi
Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils
title Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils
title_full Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils
title_fullStr Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils
title_full_unstemmed Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils
title_short Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils
title_sort isolation and screening of indigenous plant growth-promoting rhizobacteria from different rice cultivars in afghanistan soils
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934389/
https://www.ncbi.nlm.nih.gov/pubmed/31527341
http://dx.doi.org/10.1264/jsme2.ME18168
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