Cargando…
Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils
To develop biofertilizers for rice in Afghanistan, 98 plant growth-promoting rhizobacteria were isolated from rice plants and their morphological and physiological characteristics, such as indole-3-acetic acid production, acetylene reduction, phosphate and potassium solubilization, and siderophore p...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Microbial Ecology (JSME)/Japanese Society of Soil Microbiology (JSSM)/Taiwan Society of Microbial Ecology (TSME)/Japanese Society of Plant Microbe Interactions (JSPMI)/Japanese Society for Extremophiles (JSE)
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934389/ https://www.ncbi.nlm.nih.gov/pubmed/31527341 http://dx.doi.org/10.1264/jsme2.ME18168 |
_version_ | 1783483376754229248 |
---|---|
author | Habibi, Safiullah Djedidi, Salem Ohkama-Ohtsu, Naoko Sarhadi, Wakil Ahmad Kojima, Katsuhiro Rallos, Roland V. Ramirez, Maria Daniela Artigas Yamaya, Hiroko Sekimoto, Hitoshi Yokoyama, Tadashi |
author_facet | Habibi, Safiullah Djedidi, Salem Ohkama-Ohtsu, Naoko Sarhadi, Wakil Ahmad Kojima, Katsuhiro Rallos, Roland V. Ramirez, Maria Daniela Artigas Yamaya, Hiroko Sekimoto, Hitoshi Yokoyama, Tadashi |
author_sort | Habibi, Safiullah |
collection | PubMed |
description | To develop biofertilizers for rice in Afghanistan, 98 plant growth-promoting rhizobacteria were isolated from rice plants and their morphological and physiological characteristics, such as indole-3-acetic acid production, acetylene reduction, phosphate and potassium solubilization, and siderophore production, were evaluated. The genetic diversity of these bacteria was also analyzed based on 16S rRNA gene sequences. Of 98 bacteria, 89.7% produced IAA, 54.0% exhibited nitrogenase activity, and 40% showed phosphate solubilization and siderophore production. Some isolates assigned to Pseudomonas (brassicacearum, chengduensis, plecoglossicida, resinovorans, and straminea) formed a relationship with rice, and P. resinovorans and P. straminea showed nitrogen fixation. Rhizobium borbori and R. rosettiformans showed a relationship with rice plants and nitrogen fixation. Among the isolates examined, AF134 and AF137 belonging to Enterobacter ludwigii and P. putida produced large amounts of IAA (92.3 μg mL(−1)) and exhibited high nitrogenase activity (647.4 nmol C(2)H(4) h(−1)), respectively. In the plant growth test, more than 70% of the inoculated isolates showed significantly increased root and shoot dry weights. Highly diverse bacterial isolates showing promising rice growth-promoting traits were obtained from Afghanistan alkaline soils. |
format | Online Article Text |
id | pubmed-6934389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Japanese Society of Microbial Ecology (JSME)/Japanese Society of Soil Microbiology (JSSM)/Taiwan Society of Microbial Ecology (TSME)/Japanese Society of Plant Microbe Interactions (JSPMI)/Japanese Society for Extremophiles (JSE) |
record_format | MEDLINE/PubMed |
spelling | pubmed-69343892020-01-02 Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils Habibi, Safiullah Djedidi, Salem Ohkama-Ohtsu, Naoko Sarhadi, Wakil Ahmad Kojima, Katsuhiro Rallos, Roland V. Ramirez, Maria Daniela Artigas Yamaya, Hiroko Sekimoto, Hitoshi Yokoyama, Tadashi Microbes Environ Articles To develop biofertilizers for rice in Afghanistan, 98 plant growth-promoting rhizobacteria were isolated from rice plants and their morphological and physiological characteristics, such as indole-3-acetic acid production, acetylene reduction, phosphate and potassium solubilization, and siderophore production, were evaluated. The genetic diversity of these bacteria was also analyzed based on 16S rRNA gene sequences. Of 98 bacteria, 89.7% produced IAA, 54.0% exhibited nitrogenase activity, and 40% showed phosphate solubilization and siderophore production. Some isolates assigned to Pseudomonas (brassicacearum, chengduensis, plecoglossicida, resinovorans, and straminea) formed a relationship with rice, and P. resinovorans and P. straminea showed nitrogen fixation. Rhizobium borbori and R. rosettiformans showed a relationship with rice plants and nitrogen fixation. Among the isolates examined, AF134 and AF137 belonging to Enterobacter ludwigii and P. putida produced large amounts of IAA (92.3 μg mL(−1)) and exhibited high nitrogenase activity (647.4 nmol C(2)H(4) h(−1)), respectively. In the plant growth test, more than 70% of the inoculated isolates showed significantly increased root and shoot dry weights. Highly diverse bacterial isolates showing promising rice growth-promoting traits were obtained from Afghanistan alkaline soils. Japanese Society of Microbial Ecology (JSME)/Japanese Society of Soil Microbiology (JSSM)/Taiwan Society of Microbial Ecology (TSME)/Japanese Society of Plant Microbe Interactions (JSPMI)/Japanese Society for Extremophiles (JSE) 2019-12 2019-12-27 /pmc/articles/PMC6934389/ /pubmed/31527341 http://dx.doi.org/10.1264/jsme2.ME18168 Text en Copyright © 2019 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Habibi, Safiullah Djedidi, Salem Ohkama-Ohtsu, Naoko Sarhadi, Wakil Ahmad Kojima, Katsuhiro Rallos, Roland V. Ramirez, Maria Daniela Artigas Yamaya, Hiroko Sekimoto, Hitoshi Yokoyama, Tadashi Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils |
title | Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils |
title_full | Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils |
title_fullStr | Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils |
title_full_unstemmed | Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils |
title_short | Isolation and Screening of Indigenous Plant Growth-promoting Rhizobacteria from Different Rice Cultivars in Afghanistan Soils |
title_sort | isolation and screening of indigenous plant growth-promoting rhizobacteria from different rice cultivars in afghanistan soils |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934389/ https://www.ncbi.nlm.nih.gov/pubmed/31527341 http://dx.doi.org/10.1264/jsme2.ME18168 |
work_keys_str_mv | AT habibisafiullah isolationandscreeningofindigenousplantgrowthpromotingrhizobacteriafromdifferentricecultivarsinafghanistansoils AT djedidisalem isolationandscreeningofindigenousplantgrowthpromotingrhizobacteriafromdifferentricecultivarsinafghanistansoils AT ohkamaohtsunaoko isolationandscreeningofindigenousplantgrowthpromotingrhizobacteriafromdifferentricecultivarsinafghanistansoils AT sarhadiwakilahmad isolationandscreeningofindigenousplantgrowthpromotingrhizobacteriafromdifferentricecultivarsinafghanistansoils AT kojimakatsuhiro isolationandscreeningofindigenousplantgrowthpromotingrhizobacteriafromdifferentricecultivarsinafghanistansoils AT rallosrolandv isolationandscreeningofindigenousplantgrowthpromotingrhizobacteriafromdifferentricecultivarsinafghanistansoils AT ramirezmariadanielaartigas isolationandscreeningofindigenousplantgrowthpromotingrhizobacteriafromdifferentricecultivarsinafghanistansoils AT yamayahiroko isolationandscreeningofindigenousplantgrowthpromotingrhizobacteriafromdifferentricecultivarsinafghanistansoils AT sekimotohitoshi isolationandscreeningofindigenousplantgrowthpromotingrhizobacteriafromdifferentricecultivarsinafghanistansoils AT yokoyamatadashi isolationandscreeningofindigenousplantgrowthpromotingrhizobacteriafromdifferentricecultivarsinafghanistansoils |