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Impact of spaceflight on the murine thymus and mitigation by exposure to artificial gravity during spaceflight

The environment experienced during spaceflight may impact the immune system and the thymus appears to undergo atrophy during spaceflight. However, molecular aspects of this thymic atrophy remain to be elucidated. In this study, we analysed the thymi of mice on board the international space station (...

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Autores principales: Horie, Kenta, Kato, Tamotsu, Kudo, Takashi, Sasanuma, Hiroki, Miyauchi, Maki, Akiyama, Nobuko, Miyao, Takahisa, Seki, Takao, Ishikawa, Tatsuya, Takakura, Yuki, Shirakawa, Masaki, Shiba, Dai, Hamada, Michito, Jeon, Hyojung, Yoshida, Nobuaki, Inoue, Jun-ichiro, Muratani, Masafumi, Takahashi, Satoru, Ohno, Hiroshi, Akiyama, Taishin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934594/
https://www.ncbi.nlm.nih.gov/pubmed/31882694
http://dx.doi.org/10.1038/s41598-019-56432-9
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author Horie, Kenta
Kato, Tamotsu
Kudo, Takashi
Sasanuma, Hiroki
Miyauchi, Maki
Akiyama, Nobuko
Miyao, Takahisa
Seki, Takao
Ishikawa, Tatsuya
Takakura, Yuki
Shirakawa, Masaki
Shiba, Dai
Hamada, Michito
Jeon, Hyojung
Yoshida, Nobuaki
Inoue, Jun-ichiro
Muratani, Masafumi
Takahashi, Satoru
Ohno, Hiroshi
Akiyama, Taishin
author_facet Horie, Kenta
Kato, Tamotsu
Kudo, Takashi
Sasanuma, Hiroki
Miyauchi, Maki
Akiyama, Nobuko
Miyao, Takahisa
Seki, Takao
Ishikawa, Tatsuya
Takakura, Yuki
Shirakawa, Masaki
Shiba, Dai
Hamada, Michito
Jeon, Hyojung
Yoshida, Nobuaki
Inoue, Jun-ichiro
Muratani, Masafumi
Takahashi, Satoru
Ohno, Hiroshi
Akiyama, Taishin
author_sort Horie, Kenta
collection PubMed
description The environment experienced during spaceflight may impact the immune system and the thymus appears to undergo atrophy during spaceflight. However, molecular aspects of this thymic atrophy remain to be elucidated. In this study, we analysed the thymi of mice on board the international space station (ISS) for approximately 1 month. Thymic size was significantly reduced after spaceflight. Notably, exposure of mice to 1 × g using centrifugation cages in the ISS significantly mitigated the reduction in thymic size. Although spaceflight caused thymic atrophy, the global thymic structure was not largely changed. However, RNA sequencing analysis of the thymus showed significantly reduced expression of cell cycle-regulating genes in two independent spaceflight samples. These reductions were partially countered by 1 × g exposure during the space flights. Thus, our data suggest that spaceflight leads to reduced proliferation of thymic cells, thereby reducing the size of the thymus, and exposure to 1 × g might alleviate the impairment of thymus homeostasis induced by spaceflight.
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spelling pubmed-69345942019-12-29 Impact of spaceflight on the murine thymus and mitigation by exposure to artificial gravity during spaceflight Horie, Kenta Kato, Tamotsu Kudo, Takashi Sasanuma, Hiroki Miyauchi, Maki Akiyama, Nobuko Miyao, Takahisa Seki, Takao Ishikawa, Tatsuya Takakura, Yuki Shirakawa, Masaki Shiba, Dai Hamada, Michito Jeon, Hyojung Yoshida, Nobuaki Inoue, Jun-ichiro Muratani, Masafumi Takahashi, Satoru Ohno, Hiroshi Akiyama, Taishin Sci Rep Article The environment experienced during spaceflight may impact the immune system and the thymus appears to undergo atrophy during spaceflight. However, molecular aspects of this thymic atrophy remain to be elucidated. In this study, we analysed the thymi of mice on board the international space station (ISS) for approximately 1 month. Thymic size was significantly reduced after spaceflight. Notably, exposure of mice to 1 × g using centrifugation cages in the ISS significantly mitigated the reduction in thymic size. Although spaceflight caused thymic atrophy, the global thymic structure was not largely changed. However, RNA sequencing analysis of the thymus showed significantly reduced expression of cell cycle-regulating genes in two independent spaceflight samples. These reductions were partially countered by 1 × g exposure during the space flights. Thus, our data suggest that spaceflight leads to reduced proliferation of thymic cells, thereby reducing the size of the thymus, and exposure to 1 × g might alleviate the impairment of thymus homeostasis induced by spaceflight. Nature Publishing Group UK 2019-12-27 /pmc/articles/PMC6934594/ /pubmed/31882694 http://dx.doi.org/10.1038/s41598-019-56432-9 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Horie, Kenta
Kato, Tamotsu
Kudo, Takashi
Sasanuma, Hiroki
Miyauchi, Maki
Akiyama, Nobuko
Miyao, Takahisa
Seki, Takao
Ishikawa, Tatsuya
Takakura, Yuki
Shirakawa, Masaki
Shiba, Dai
Hamada, Michito
Jeon, Hyojung
Yoshida, Nobuaki
Inoue, Jun-ichiro
Muratani, Masafumi
Takahashi, Satoru
Ohno, Hiroshi
Akiyama, Taishin
Impact of spaceflight on the murine thymus and mitigation by exposure to artificial gravity during spaceflight
title Impact of spaceflight on the murine thymus and mitigation by exposure to artificial gravity during spaceflight
title_full Impact of spaceflight on the murine thymus and mitigation by exposure to artificial gravity during spaceflight
title_fullStr Impact of spaceflight on the murine thymus and mitigation by exposure to artificial gravity during spaceflight
title_full_unstemmed Impact of spaceflight on the murine thymus and mitigation by exposure to artificial gravity during spaceflight
title_short Impact of spaceflight on the murine thymus and mitigation by exposure to artificial gravity during spaceflight
title_sort impact of spaceflight on the murine thymus and mitigation by exposure to artificial gravity during spaceflight
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934594/
https://www.ncbi.nlm.nih.gov/pubmed/31882694
http://dx.doi.org/10.1038/s41598-019-56432-9
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