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Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography
Large or complex bone fractures often need clinical treatments for sufficient bone repair. New treatment strategies have pursued the idea of using mesenchymal stromal cells (MSCs) in combination with osteoinductive materials to guide differentiation of MSCs into bone cells ensuring complete bone reg...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934613/ https://www.ncbi.nlm.nih.gov/pubmed/31882795 http://dx.doi.org/10.1038/s41598-019-56508-6 |
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author | Raic, Annamarija Friedrich, Frank Kratzer, Domenic Bieback, Karen Lahann, Joerg Lee-Thedieck, Cornelia |
author_facet | Raic, Annamarija Friedrich, Frank Kratzer, Domenic Bieback, Karen Lahann, Joerg Lee-Thedieck, Cornelia |
author_sort | Raic, Annamarija |
collection | PubMed |
description | Large or complex bone fractures often need clinical treatments for sufficient bone repair. New treatment strategies have pursued the idea of using mesenchymal stromal cells (MSCs) in combination with osteoinductive materials to guide differentiation of MSCs into bone cells ensuring complete bone regeneration. To overcome the challenge of developing such materials, fundamental studies are needed to analyze and understand the MSC behavior on modified surfaces of applicable materials for bone healing. For this purpose, we developed a fibrous scaffold resembling the bone/bone marrow extracellular matrix (ECM) based on protein without addition of synthetic polymers. With this biomimetic in vitro model we identified the fibrous structure as well as the charge of the material to be responsible for its effects on MSC differentiation. Positive charge was introduced via cationization that additionally supported the stability of the scaffold in cell culture, and acted as nucleation point for mineralization during osteogenesis. Furthermore, we revealed enhanced focal adhesion formation and osteogenic differentiation of MSCs cultured on positively charged protein fibers. This pure protein-based and chemically modifiable, fibrous ECM model allows the investigation of MSC behavior on biomimetic materials to unfold new vistas how to direct cells’ differentiation for the development of new bone regenerating strategies. |
format | Online Article Text |
id | pubmed-6934613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69346132019-12-30 Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography Raic, Annamarija Friedrich, Frank Kratzer, Domenic Bieback, Karen Lahann, Joerg Lee-Thedieck, Cornelia Sci Rep Article Large or complex bone fractures often need clinical treatments for sufficient bone repair. New treatment strategies have pursued the idea of using mesenchymal stromal cells (MSCs) in combination with osteoinductive materials to guide differentiation of MSCs into bone cells ensuring complete bone regeneration. To overcome the challenge of developing such materials, fundamental studies are needed to analyze and understand the MSC behavior on modified surfaces of applicable materials for bone healing. For this purpose, we developed a fibrous scaffold resembling the bone/bone marrow extracellular matrix (ECM) based on protein without addition of synthetic polymers. With this biomimetic in vitro model we identified the fibrous structure as well as the charge of the material to be responsible for its effects on MSC differentiation. Positive charge was introduced via cationization that additionally supported the stability of the scaffold in cell culture, and acted as nucleation point for mineralization during osteogenesis. Furthermore, we revealed enhanced focal adhesion formation and osteogenic differentiation of MSCs cultured on positively charged protein fibers. This pure protein-based and chemically modifiable, fibrous ECM model allows the investigation of MSC behavior on biomimetic materials to unfold new vistas how to direct cells’ differentiation for the development of new bone regenerating strategies. Nature Publishing Group UK 2019-12-27 /pmc/articles/PMC6934613/ /pubmed/31882795 http://dx.doi.org/10.1038/s41598-019-56508-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Raic, Annamarija Friedrich, Frank Kratzer, Domenic Bieback, Karen Lahann, Joerg Lee-Thedieck, Cornelia Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography |
title | Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography |
title_full | Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography |
title_fullStr | Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography |
title_full_unstemmed | Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography |
title_short | Potential of electrospun cationic BSA fibers to guide osteogenic MSC differentiation via surface charge and fibrous topography |
title_sort | potential of electrospun cationic bsa fibers to guide osteogenic msc differentiation via surface charge and fibrous topography |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934613/ https://www.ncbi.nlm.nih.gov/pubmed/31882795 http://dx.doi.org/10.1038/s41598-019-56508-6 |
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