Microscale pressure measurements based on an immiscible fluid/fluid interface

A method of microscale pressure measurement based on immiscible fluid/fluid interface is proposed. This method utilizes observed curvature changes in a fluid/fluid interface, and can accurately report hydraulic pressure in fluids at length scales of 10 microns. The method is especially suited for me...

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Autores principales: Yang, Jing, Duan, Xing, Fraser, Andrew K., Choudhury, Mohammad Ikbal, Ewald, Andrew J., Li, Rong, Sun, Sean X.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934680/
https://www.ncbi.nlm.nih.gov/pubmed/31882951
http://dx.doi.org/10.1038/s41598-019-56573-x
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author Yang, Jing
Duan, Xing
Fraser, Andrew K.
Choudhury, Mohammad Ikbal
Ewald, Andrew J.
Li, Rong
Sun, Sean X.
author_facet Yang, Jing
Duan, Xing
Fraser, Andrew K.
Choudhury, Mohammad Ikbal
Ewald, Andrew J.
Li, Rong
Sun, Sean X.
author_sort Yang, Jing
collection PubMed
description A method of microscale pressure measurement based on immiscible fluid/fluid interface is proposed. This method utilizes observed curvature changes in a fluid/fluid interface, and can accurately report hydraulic pressure in fluids at length scales of 10 microns. The method is especially suited for measuring fluid pressure in micro-scale biological samples. Using this method, we probe fluid pressure build up in epithelial domes, murine mammary gland organoids embedded in hydrogel, and lumen pressure in the developing mouse embryo. Results reveal that the pressure developed across epithelial barriers is on the order of 100~300 Pa, and is modulated by ion channel activity.
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spelling pubmed-69346802019-12-30 Microscale pressure measurements based on an immiscible fluid/fluid interface Yang, Jing Duan, Xing Fraser, Andrew K. Choudhury, Mohammad Ikbal Ewald, Andrew J. Li, Rong Sun, Sean X. Sci Rep Article A method of microscale pressure measurement based on immiscible fluid/fluid interface is proposed. This method utilizes observed curvature changes in a fluid/fluid interface, and can accurately report hydraulic pressure in fluids at length scales of 10 microns. The method is especially suited for measuring fluid pressure in micro-scale biological samples. Using this method, we probe fluid pressure build up in epithelial domes, murine mammary gland organoids embedded in hydrogel, and lumen pressure in the developing mouse embryo. Results reveal that the pressure developed across epithelial barriers is on the order of 100~300 Pa, and is modulated by ion channel activity. Nature Publishing Group UK 2019-12-27 /pmc/articles/PMC6934680/ /pubmed/31882951 http://dx.doi.org/10.1038/s41598-019-56573-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yang, Jing
Duan, Xing
Fraser, Andrew K.
Choudhury, Mohammad Ikbal
Ewald, Andrew J.
Li, Rong
Sun, Sean X.
Microscale pressure measurements based on an immiscible fluid/fluid interface
title Microscale pressure measurements based on an immiscible fluid/fluid interface
title_full Microscale pressure measurements based on an immiscible fluid/fluid interface
title_fullStr Microscale pressure measurements based on an immiscible fluid/fluid interface
title_full_unstemmed Microscale pressure measurements based on an immiscible fluid/fluid interface
title_short Microscale pressure measurements based on an immiscible fluid/fluid interface
title_sort microscale pressure measurements based on an immiscible fluid/fluid interface
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934680/
https://www.ncbi.nlm.nih.gov/pubmed/31882951
http://dx.doi.org/10.1038/s41598-019-56573-x
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