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Effects of cold exposure revealed by global transcriptomic analysis in ferret peripheral blood mononuclear cells

Animal studies, mostly performed in rodents, show the beneficial anti-obesity effects of cold studies. This is due to thermogenic activation of brown adipose tissue (BAT), a tissue also recently discovered in adult humans. Studies in humans, however, are hampered by the accessibility of most tissues...

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Autores principales: Reynés, Bàrbara, van Schothorst, Evert M., Keijer, Jaap, Palou, Andreu, Oliver, Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934835/
https://www.ncbi.nlm.nih.gov/pubmed/31882687
http://dx.doi.org/10.1038/s41598-019-56354-6
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author Reynés, Bàrbara
van Schothorst, Evert M.
Keijer, Jaap
Palou, Andreu
Oliver, Paula
author_facet Reynés, Bàrbara
van Schothorst, Evert M.
Keijer, Jaap
Palou, Andreu
Oliver, Paula
author_sort Reynés, Bàrbara
collection PubMed
description Animal studies, mostly performed in rodents, show the beneficial anti-obesity effects of cold studies. This is due to thermogenic activation of brown adipose tissue (BAT), a tissue also recently discovered in adult humans. Studies in humans, however, are hampered by the accessibility of most tissues. In contrast, peripheral blood mononuclear cells (PBMC) are accessible and share the expression profile of different sets of genes with other tissues, including those that reflect metabolic responses. Ferrets are an animal model physiologically closer to humans than rodents. Here, we investigated the effects on ferrets of one-week acclimation to 4 °C by analysing the PBMC transcriptome. Cold exposure deeply affected PBMC gene expression, producing a widespread down-regulation of genes involved in different biological pathways (cell cycle, gene expression regulation/protein synthesis, immune response, signal transduction, and genes related to extracellular matrix/cytoskeleton), while thermogenic and glycogenolysis-related processes were increased. Results obtained in PBMC reflected those of adipose tissue, but hardly those of the liver. Our study, using ferret as a model, reinforce PBMC usefulness as sentinel biological material for cold-exposure studies in order to deepen our understanding of the general and specific pathways affected by cold acclimation. This is relevant for future development of therapies to be used clinically.
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spelling pubmed-69348352019-12-31 Effects of cold exposure revealed by global transcriptomic analysis in ferret peripheral blood mononuclear cells Reynés, Bàrbara van Schothorst, Evert M. Keijer, Jaap Palou, Andreu Oliver, Paula Sci Rep Article Animal studies, mostly performed in rodents, show the beneficial anti-obesity effects of cold studies. This is due to thermogenic activation of brown adipose tissue (BAT), a tissue also recently discovered in adult humans. Studies in humans, however, are hampered by the accessibility of most tissues. In contrast, peripheral blood mononuclear cells (PBMC) are accessible and share the expression profile of different sets of genes with other tissues, including those that reflect metabolic responses. Ferrets are an animal model physiologically closer to humans than rodents. Here, we investigated the effects on ferrets of one-week acclimation to 4 °C by analysing the PBMC transcriptome. Cold exposure deeply affected PBMC gene expression, producing a widespread down-regulation of genes involved in different biological pathways (cell cycle, gene expression regulation/protein synthesis, immune response, signal transduction, and genes related to extracellular matrix/cytoskeleton), while thermogenic and glycogenolysis-related processes were increased. Results obtained in PBMC reflected those of adipose tissue, but hardly those of the liver. Our study, using ferret as a model, reinforce PBMC usefulness as sentinel biological material for cold-exposure studies in order to deepen our understanding of the general and specific pathways affected by cold acclimation. This is relevant for future development of therapies to be used clinically. Nature Publishing Group UK 2019-12-27 /pmc/articles/PMC6934835/ /pubmed/31882687 http://dx.doi.org/10.1038/s41598-019-56354-6 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Reynés, Bàrbara
van Schothorst, Evert M.
Keijer, Jaap
Palou, Andreu
Oliver, Paula
Effects of cold exposure revealed by global transcriptomic analysis in ferret peripheral blood mononuclear cells
title Effects of cold exposure revealed by global transcriptomic analysis in ferret peripheral blood mononuclear cells
title_full Effects of cold exposure revealed by global transcriptomic analysis in ferret peripheral blood mononuclear cells
title_fullStr Effects of cold exposure revealed by global transcriptomic analysis in ferret peripheral blood mononuclear cells
title_full_unstemmed Effects of cold exposure revealed by global transcriptomic analysis in ferret peripheral blood mononuclear cells
title_short Effects of cold exposure revealed by global transcriptomic analysis in ferret peripheral blood mononuclear cells
title_sort effects of cold exposure revealed by global transcriptomic analysis in ferret peripheral blood mononuclear cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934835/
https://www.ncbi.nlm.nih.gov/pubmed/31882687
http://dx.doi.org/10.1038/s41598-019-56354-6
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