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Elevated expression of AGGF1 predicts poor prognosis and promotes the metastasis of colorectal cancer
BACKGROUND: Angiogenic factor with G-patch and FHA domains 1 (AGGF1) can promote angiogenesis and increasing evidence has highlighted the important roles of AGGF1 in tumorigenesis. However, the differential expression as well as the biological functions of AGGF1 in colorectal cancer (CRC) remain to...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935059/ https://www.ncbi.nlm.nih.gov/pubmed/31881864 http://dx.doi.org/10.1186/s12885-019-6474-7 |
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author | Zhang, Xin Sun, Huimin Chen, Wanyuan He, Xianglei |
author_facet | Zhang, Xin Sun, Huimin Chen, Wanyuan He, Xianglei |
author_sort | Zhang, Xin |
collection | PubMed |
description | BACKGROUND: Angiogenic factor with G-patch and FHA domains 1 (AGGF1) can promote angiogenesis and increasing evidence has highlighted the important roles of AGGF1 in tumorigenesis. However, the differential expression as well as the biological functions of AGGF1 in colorectal cancer (CRC) remain to be established. The purpose of the present study is therefore to identify the effect of AGGF1 on prognosis and metastasis in CRC patients. METHODS: The expression level of AGGF1 in CRC was examined by qPCR, western blot and immunohistochemistry in a tissue microarray containing 236 CRC specimens and paired normal mucosae. And the effect of AGGF1 on CRC cell malignance was investigated in our established stable AGGF1 upregulated and knockdown CRC cell lines. RESULTS: The expression level of AGGF1 in CRC tissue was not significantly different to that in adjacent normal mucosa at the mRNA level. However, at the protein level, AGGF1 expression in CRC tissues was significantly higher than in paired normal mucosa, which showed a clear association with TNM stage, AJCC stage, vascular invasion, and differentiation. Further, we revealed an apparent correlation between AGGF1 expression and poorer disease-free survival and overall survival of CRC patients. In addition, we discovered that AGGF1 significantly promoted CRC cell wound healing, migration, and invasion in vitro and distant metastasis in vivo. CONCLUSIONS: Our study demonstrates the aberrant overexpression of AGGF1 in CRC and provides a basis on which to explore the application of AGGF1 as a potential therapeutic target for CRC patients, especially for CRC patients with distant metastasis. |
format | Online Article Text |
id | pubmed-6935059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69350592019-12-30 Elevated expression of AGGF1 predicts poor prognosis and promotes the metastasis of colorectal cancer Zhang, Xin Sun, Huimin Chen, Wanyuan He, Xianglei BMC Cancer Research Article BACKGROUND: Angiogenic factor with G-patch and FHA domains 1 (AGGF1) can promote angiogenesis and increasing evidence has highlighted the important roles of AGGF1 in tumorigenesis. However, the differential expression as well as the biological functions of AGGF1 in colorectal cancer (CRC) remain to be established. The purpose of the present study is therefore to identify the effect of AGGF1 on prognosis and metastasis in CRC patients. METHODS: The expression level of AGGF1 in CRC was examined by qPCR, western blot and immunohistochemistry in a tissue microarray containing 236 CRC specimens and paired normal mucosae. And the effect of AGGF1 on CRC cell malignance was investigated in our established stable AGGF1 upregulated and knockdown CRC cell lines. RESULTS: The expression level of AGGF1 in CRC tissue was not significantly different to that in adjacent normal mucosa at the mRNA level. However, at the protein level, AGGF1 expression in CRC tissues was significantly higher than in paired normal mucosa, which showed a clear association with TNM stage, AJCC stage, vascular invasion, and differentiation. Further, we revealed an apparent correlation between AGGF1 expression and poorer disease-free survival and overall survival of CRC patients. In addition, we discovered that AGGF1 significantly promoted CRC cell wound healing, migration, and invasion in vitro and distant metastasis in vivo. CONCLUSIONS: Our study demonstrates the aberrant overexpression of AGGF1 in CRC and provides a basis on which to explore the application of AGGF1 as a potential therapeutic target for CRC patients, especially for CRC patients with distant metastasis. BioMed Central 2019-12-27 /pmc/articles/PMC6935059/ /pubmed/31881864 http://dx.doi.org/10.1186/s12885-019-6474-7 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Zhang, Xin Sun, Huimin Chen, Wanyuan He, Xianglei Elevated expression of AGGF1 predicts poor prognosis and promotes the metastasis of colorectal cancer |
title | Elevated expression of AGGF1 predicts poor prognosis and promotes the metastasis of colorectal cancer |
title_full | Elevated expression of AGGF1 predicts poor prognosis and promotes the metastasis of colorectal cancer |
title_fullStr | Elevated expression of AGGF1 predicts poor prognosis and promotes the metastasis of colorectal cancer |
title_full_unstemmed | Elevated expression of AGGF1 predicts poor prognosis and promotes the metastasis of colorectal cancer |
title_short | Elevated expression of AGGF1 predicts poor prognosis and promotes the metastasis of colorectal cancer |
title_sort | elevated expression of aggf1 predicts poor prognosis and promotes the metastasis of colorectal cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935059/ https://www.ncbi.nlm.nih.gov/pubmed/31881864 http://dx.doi.org/10.1186/s12885-019-6474-7 |
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