Cargando…
Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region
BACKGROUND: Short internodes contribute to plant dwarfism, which is exceedingly beneficial for crop production. However, the underlying mechanisms of internode elongation are complicated and have been not fully understood. RESULTS: Here, we report a maize dwarf mutant, dwarf2014 (d2014), which displ...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935237/ https://www.ncbi.nlm.nih.gov/pubmed/31881837 http://dx.doi.org/10.1186/s12870-019-2200-5 |
_version_ | 1783483548893708288 |
---|---|
author | Zhang, Xiangge Hou, Xianbin Liu, Yinghong Zheng, Lanjie Yi, Qiang Zhang, Haojun Huang, Xinrong Zhang, Junjie Hu, Yufeng Yu, Guowu Liu, Hanmei Li, Yangping Huang, Huanhuan Zhan, Feilong Chen, Lin Tang, Jihua Huang, Yubi |
author_facet | Zhang, Xiangge Hou, Xianbin Liu, Yinghong Zheng, Lanjie Yi, Qiang Zhang, Haojun Huang, Xinrong Zhang, Junjie Hu, Yufeng Yu, Guowu Liu, Hanmei Li, Yangping Huang, Huanhuan Zhan, Feilong Chen, Lin Tang, Jihua Huang, Yubi |
author_sort | Zhang, Xiangge |
collection | PubMed |
description | BACKGROUND: Short internodes contribute to plant dwarfism, which is exceedingly beneficial for crop production. However, the underlying mechanisms of internode elongation are complicated and have been not fully understood. RESULTS: Here, we report a maize dwarf mutant, dwarf2014 (d2014), which displays shortened lower internodes. Map-based cloning revealed that the d2014 gene is a novel br2 allele with a splicing variation, resulting in a higher expression of BR2-T02 instead of normal BR2-T01. Then, we found that the internode elongation in d2014/br2 exhibited a pattern of inhibition-normality-inhibition (transient for the ear-internode), correspondingly, at the 6-leaf, 12-leaf and 14-leaf stages. Indeed, BR2 encodes a P-glycoprotein1 (PGP1) protein that functions in auxin efflux, and our in situ hybridization assay showed that BR2 was mainly expressed in vascular bundles of the node and internode. Furthermore, significantly higher auxin concentration was detected in the stem apex of d2014 at the 6-leaf stage and strictly in the node region for the ear-internode at the 14-leaf stage. In such context, we propose that BR2/PGP1 transports auxin from node to internode through the vascular bundles, and excessive auxin accumulation in the node (immediately next to the intercalary meristem) region suppresses internode elongation of d2014. CONCLUSIONS: These findings suggest that low auxin levels mediated by BR2/PGP1 in the intercalary meristem region are crucial for internode elongation. |
format | Online Article Text |
id | pubmed-6935237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69352372019-12-30 Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region Zhang, Xiangge Hou, Xianbin Liu, Yinghong Zheng, Lanjie Yi, Qiang Zhang, Haojun Huang, Xinrong Zhang, Junjie Hu, Yufeng Yu, Guowu Liu, Hanmei Li, Yangping Huang, Huanhuan Zhan, Feilong Chen, Lin Tang, Jihua Huang, Yubi BMC Plant Biol Research Article BACKGROUND: Short internodes contribute to plant dwarfism, which is exceedingly beneficial for crop production. However, the underlying mechanisms of internode elongation are complicated and have been not fully understood. RESULTS: Here, we report a maize dwarf mutant, dwarf2014 (d2014), which displays shortened lower internodes. Map-based cloning revealed that the d2014 gene is a novel br2 allele with a splicing variation, resulting in a higher expression of BR2-T02 instead of normal BR2-T01. Then, we found that the internode elongation in d2014/br2 exhibited a pattern of inhibition-normality-inhibition (transient for the ear-internode), correspondingly, at the 6-leaf, 12-leaf and 14-leaf stages. Indeed, BR2 encodes a P-glycoprotein1 (PGP1) protein that functions in auxin efflux, and our in situ hybridization assay showed that BR2 was mainly expressed in vascular bundles of the node and internode. Furthermore, significantly higher auxin concentration was detected in the stem apex of d2014 at the 6-leaf stage and strictly in the node region for the ear-internode at the 14-leaf stage. In such context, we propose that BR2/PGP1 transports auxin from node to internode through the vascular bundles, and excessive auxin accumulation in the node (immediately next to the intercalary meristem) region suppresses internode elongation of d2014. CONCLUSIONS: These findings suggest that low auxin levels mediated by BR2/PGP1 in the intercalary meristem region are crucial for internode elongation. BioMed Central 2019-12-27 /pmc/articles/PMC6935237/ /pubmed/31881837 http://dx.doi.org/10.1186/s12870-019-2200-5 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Zhang, Xiangge Hou, Xianbin Liu, Yinghong Zheng, Lanjie Yi, Qiang Zhang, Haojun Huang, Xinrong Zhang, Junjie Hu, Yufeng Yu, Guowu Liu, Hanmei Li, Yangping Huang, Huanhuan Zhan, Feilong Chen, Lin Tang, Jihua Huang, Yubi Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region |
title | Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region |
title_full | Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region |
title_fullStr | Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region |
title_full_unstemmed | Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region |
title_short | Maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region |
title_sort | maize brachytic2 (br2) suppresses the elongation of lower internodes for excessive auxin accumulation in the intercalary meristem region |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6935237/ https://www.ncbi.nlm.nih.gov/pubmed/31881837 http://dx.doi.org/10.1186/s12870-019-2200-5 |
work_keys_str_mv | AT zhangxiangge maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT houxianbin maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT liuyinghong maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT zhenglanjie maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT yiqiang maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT zhanghaojun maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT huangxinrong maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT zhangjunjie maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT huyufeng maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT yuguowu maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT liuhanmei maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT liyangping maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT huanghuanhuan maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT zhanfeilong maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT chenlin maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT tangjihua maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion AT huangyubi maizebrachytic2br2suppressestheelongationoflowerinternodesforexcessiveauxinaccumulationintheintercalarymeristemregion |