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Expression analysis of Rab11 during zebrafish embryonic development

BACKGROUND: Rab proteins are GTPases responsible for intracellular vesicular trafficking regulation. Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. In zebrafish, there are 3 rab11 paralogues, known as rab11a, rab11ba and rab1...

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Autores principales: Zhang, Haijun, Gao, Yu, Qian, Peipei, Dong, Zhangji, Hao, Wenjin, Liu, Dong, Duan, Xuchu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936149/
https://www.ncbi.nlm.nih.gov/pubmed/31884948
http://dx.doi.org/10.1186/s12861-019-0207-7
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author Zhang, Haijun
Gao, Yu
Qian, Peipei
Dong, Zhangji
Hao, Wenjin
Liu, Dong
Duan, Xuchu
author_facet Zhang, Haijun
Gao, Yu
Qian, Peipei
Dong, Zhangji
Hao, Wenjin
Liu, Dong
Duan, Xuchu
author_sort Zhang, Haijun
collection PubMed
description BACKGROUND: Rab proteins are GTPases responsible for intracellular vesicular trafficking regulation. Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. In zebrafish, there are 3 rab11 paralogues, known as rab11a, rab11ba and rab11bb, sharing high identity with each other. However, the expression analysis of rab11 is so far lacking. RESULTS: Here, by phylogeny analysis, we found the three rab11 genes are highly conserved especially for their GTPase domains. We examined the expression patterns of rab11a, rab11ba and rab11bb using RT-PCR and in situ hybridization. We found that all the three genes were highly enriched in the central nervous system, but in different areas of the brain. Apart from brain, rab11a was also expressed in caudal vein, pronephric duct, proctodeum, pharyngeal arches and digestive duct, rab11ba was detected to express in muscle, and rab11bb was expressed in kidney, fin and spinal cord. Different from rab11a and rab11ba, which both have maternal expressions in embryos, rab11bb only expresses during 24hpf to 96hpf. CONCLUSIONS: Our results suggest that rab11 genes play important but distinct roles in the development of the nervous system in zebrafish. The findings could provide new evidences for better understanding the functions of rab11 in the development of zebrafish embryos.
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spelling pubmed-69361492019-12-31 Expression analysis of Rab11 during zebrafish embryonic development Zhang, Haijun Gao, Yu Qian, Peipei Dong, Zhangji Hao, Wenjin Liu, Dong Duan, Xuchu BMC Dev Biol Research Article BACKGROUND: Rab proteins are GTPases responsible for intracellular vesicular trafficking regulation. Rab11 proteins, members of the Rab GTPase family, are known to regulate vesicular recycling during embryonic development. In zebrafish, there are 3 rab11 paralogues, known as rab11a, rab11ba and rab11bb, sharing high identity with each other. However, the expression analysis of rab11 is so far lacking. RESULTS: Here, by phylogeny analysis, we found the three rab11 genes are highly conserved especially for their GTPase domains. We examined the expression patterns of rab11a, rab11ba and rab11bb using RT-PCR and in situ hybridization. We found that all the three genes were highly enriched in the central nervous system, but in different areas of the brain. Apart from brain, rab11a was also expressed in caudal vein, pronephric duct, proctodeum, pharyngeal arches and digestive duct, rab11ba was detected to express in muscle, and rab11bb was expressed in kidney, fin and spinal cord. Different from rab11a and rab11ba, which both have maternal expressions in embryos, rab11bb only expresses during 24hpf to 96hpf. CONCLUSIONS: Our results suggest that rab11 genes play important but distinct roles in the development of the nervous system in zebrafish. The findings could provide new evidences for better understanding the functions of rab11 in the development of zebrafish embryos. BioMed Central 2019-12-30 /pmc/articles/PMC6936149/ /pubmed/31884948 http://dx.doi.org/10.1186/s12861-019-0207-7 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Haijun
Gao, Yu
Qian, Peipei
Dong, Zhangji
Hao, Wenjin
Liu, Dong
Duan, Xuchu
Expression analysis of Rab11 during zebrafish embryonic development
title Expression analysis of Rab11 during zebrafish embryonic development
title_full Expression analysis of Rab11 during zebrafish embryonic development
title_fullStr Expression analysis of Rab11 during zebrafish embryonic development
title_full_unstemmed Expression analysis of Rab11 during zebrafish embryonic development
title_short Expression analysis of Rab11 during zebrafish embryonic development
title_sort expression analysis of rab11 during zebrafish embryonic development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936149/
https://www.ncbi.nlm.nih.gov/pubmed/31884948
http://dx.doi.org/10.1186/s12861-019-0207-7
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