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Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane
Alternative Splicing (AS) is a mechanism that generates different mature transcripts from precursor mRNAs (pre-mRNAs) of the same gene. In plants, a wide range of physiological and metabolic events are related to AS, as well as fast responses to changes in temperature. AS is present in around 60% of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936171/ https://www.ncbi.nlm.nih.gov/pubmed/31921258 http://dx.doi.org/10.3389/fpls.2019.01614 |
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author | Dantas, Luíza L. B. Calixto, Cristiane P. G. Dourado, Maira M. Carneiro, Monalisa S. Brown, John W. S. Hotta, Carlos T. |
author_facet | Dantas, Luíza L. B. Calixto, Cristiane P. G. Dourado, Maira M. Carneiro, Monalisa S. Brown, John W. S. Hotta, Carlos T. |
author_sort | Dantas, Luíza L. B. |
collection | PubMed |
description | Alternative Splicing (AS) is a mechanism that generates different mature transcripts from precursor mRNAs (pre-mRNAs) of the same gene. In plants, a wide range of physiological and metabolic events are related to AS, as well as fast responses to changes in temperature. AS is present in around 60% of intron-containing genes in Arabidopsis, 46% in rice, and 38% in maize and it is widespread among the circadian clock genes. Little is known about how AS influences the circadian clock of C4 plants, like commercial sugarcane, a C4 crop with a complex hybrid genome. This work aims to test if the daily dynamics of AS forms of circadian clock genes are regulated by environmental factors, such as temperature, in the field. A systematic search for AS in five sugarcane clock genes, ScLHY, ScPRR37, ScPRR73, ScPRR95, and ScTOC1 using different organs of sugarcane sampled during winter, with 4 months old plants, and during summer, with 9 months old plants, revealed temperature- and organ-dependent expression of at least one alternatively spliced isoform in all genes. Expression of AS isoforms varied according to the season. Our results suggest that AS events in circadian clock genes are correlated with temperature. |
format | Online Article Text |
id | pubmed-6936171 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69361712020-01-09 Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane Dantas, Luíza L. B. Calixto, Cristiane P. G. Dourado, Maira M. Carneiro, Monalisa S. Brown, John W. S. Hotta, Carlos T. Front Plant Sci Plant Science Alternative Splicing (AS) is a mechanism that generates different mature transcripts from precursor mRNAs (pre-mRNAs) of the same gene. In plants, a wide range of physiological and metabolic events are related to AS, as well as fast responses to changes in temperature. AS is present in around 60% of intron-containing genes in Arabidopsis, 46% in rice, and 38% in maize and it is widespread among the circadian clock genes. Little is known about how AS influences the circadian clock of C4 plants, like commercial sugarcane, a C4 crop with a complex hybrid genome. This work aims to test if the daily dynamics of AS forms of circadian clock genes are regulated by environmental factors, such as temperature, in the field. A systematic search for AS in five sugarcane clock genes, ScLHY, ScPRR37, ScPRR73, ScPRR95, and ScTOC1 using different organs of sugarcane sampled during winter, with 4 months old plants, and during summer, with 9 months old plants, revealed temperature- and organ-dependent expression of at least one alternatively spliced isoform in all genes. Expression of AS isoforms varied according to the season. Our results suggest that AS events in circadian clock genes are correlated with temperature. Frontiers Media S.A. 2019-12-23 /pmc/articles/PMC6936171/ /pubmed/31921258 http://dx.doi.org/10.3389/fpls.2019.01614 Text en Copyright © 2019 Dantas, Calixto, Dourado, Carneiro, Brown and Hotta http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Dantas, Luíza L. B. Calixto, Cristiane P. G. Dourado, Maira M. Carneiro, Monalisa S. Brown, John W. S. Hotta, Carlos T. Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane |
title | Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane |
title_full | Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane |
title_fullStr | Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane |
title_full_unstemmed | Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane |
title_short | Alternative Splicing of Circadian Clock Genes Correlates With Temperature in Field-Grown Sugarcane |
title_sort | alternative splicing of circadian clock genes correlates with temperature in field-grown sugarcane |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936171/ https://www.ncbi.nlm.nih.gov/pubmed/31921258 http://dx.doi.org/10.3389/fpls.2019.01614 |
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