tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with si...

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Autores principales: Nieves, Daniel J., Hilzenrat, Geva, Tran, Jason, Yang, Zhengmin, MacRae, Hugh H., Baker, Matthew A. B., Gooding, J. Justin, Gaus, Katharina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2019
Materias:
Biochemistry & Biophysics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936279/
https://www.ncbi.nlm.nih.gov/pubmed/31903209
http://dx.doi.org/10.1098/rsos.191268
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author Nieves, Daniel J.
Hilzenrat, Geva
Tran, Jason
Yang, Zhengmin
MacRae, Hugh H.
Baker, Matthew A. B.
Gooding, J. Justin
Gaus, Katharina
author_facet Nieves, Daniel J.
Hilzenrat, Geva
Tran, Jason
Yang, Zhengmin
MacRae, Hugh H.
Baker, Matthew A. B.
Gooding, J. Justin
Gaus, Katharina
author_sort Nieves, Daniel J.
collection PubMed
description Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle.
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spelling pubmed-69362792020-01-03 tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging Nieves, Daniel J. Hilzenrat, Geva Tran, Jason Yang, Zhengmin MacRae, Hugh H. Baker, Matthew A. B. Gooding, J. Justin Gaus, Katharina R Soc Open Sci Biochemistry & Biophysics Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with single DNA-docking strands and application of SMLM via DNA-PAINT is demonstrated. tagPAINT is then used for T-cell receptor signalling proteins at the immune synapse as a proof of principle. The Royal Society 2019-12-11 /pmc/articles/PMC6936279/ /pubmed/31903209 http://dx.doi.org/10.1098/rsos.191268 Text en © 2019 The Authors. http://creativecommons.org/licenses/by/4.0/ Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
spellingShingle Biochemistry & Biophysics
Nieves, Daniel J.
Hilzenrat, Geva
Tran, Jason
Yang, Zhengmin
MacRae, Hugh H.
Baker, Matthew A. B.
Gooding, J. Justin
Gaus, Katharina
tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging
title tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging
title_full tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging
title_fullStr tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging
title_full_unstemmed tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging
title_short tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging
title_sort tagpaint: covalent labelling of genetically encoded protein tags for dna-paint imaging
topic Biochemistry & Biophysics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936279/
https://www.ncbi.nlm.nih.gov/pubmed/31903209
http://dx.doi.org/10.1098/rsos.191268
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