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tagPAINT: covalent labelling of genetically encoded protein tags for DNA-PAINT imaging

Recently, DNA-PAINT single-molecule localization microscopy (SMLM) has shown great promise for quantitative imaging; however, labelling strategies thus far have relied on multivalent and affinity-based approaches. Here, the covalent labelling of expressed protein tags (SNAP tag and Halo tag) with si...

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Detalles Bibliográficos
Autores principales: Nieves, Daniel J., Hilzenrat, Geva, Tran, Jason, Yang, Zhengmin, MacRae, Hugh H., Baker, Matthew A. B., Gooding, J. Justin, Gaus, Katharina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936279/
https://www.ncbi.nlm.nih.gov/pubmed/31903209
http://dx.doi.org/10.1098/rsos.191268