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Insights into fungal diversity of a shallow-water hydrothermal vent field at Kueishan Island, Taiwan by culture-based and metabarcoding analyses

This paper reports the diversity of fungi associated with substrates collected at a shallow hydrothermal vent field at Kueishan Island, Taiwan, using both culture-based and metabarcoding methods. Culture of fungi from yellow sediment (with visible sulfur granules), black sediment (no visible sulfur...

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Autores principales: Pang, Ka-Lai, Guo, Sheng-Yu, Chen, I-An, Burgaud, Gäetan, Luo, Zhu-Hua, Dahms, Hans U., Hwang, Jiang-Shiou, Lin, Yi-Li, Huang, Jian-Shun, Ho, Tsz-Wai, Tsang, Ling-Ming, Chiang, Michael Wai-Lun, Cha, Hyo-Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936883/
https://www.ncbi.nlm.nih.gov/pubmed/31887170
http://dx.doi.org/10.1371/journal.pone.0226616
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author Pang, Ka-Lai
Guo, Sheng-Yu
Chen, I-An
Burgaud, Gäetan
Luo, Zhu-Hua
Dahms, Hans U.
Hwang, Jiang-Shiou
Lin, Yi-Li
Huang, Jian-Shun
Ho, Tsz-Wai
Tsang, Ling-Ming
Chiang, Michael Wai-Lun
Cha, Hyo-Jung
author_facet Pang, Ka-Lai
Guo, Sheng-Yu
Chen, I-An
Burgaud, Gäetan
Luo, Zhu-Hua
Dahms, Hans U.
Hwang, Jiang-Shiou
Lin, Yi-Li
Huang, Jian-Shun
Ho, Tsz-Wai
Tsang, Ling-Ming
Chiang, Michael Wai-Lun
Cha, Hyo-Jung
author_sort Pang, Ka-Lai
collection PubMed
description This paper reports the diversity of fungi associated with substrates collected at a shallow hydrothermal vent field at Kueishan Island, Taiwan, using both culture-based and metabarcoding methods. Culture of fungi from yellow sediment (with visible sulfur granules), black sediment (no visible sulfur granules), the vent crab Xenograpsus testudinatus, seawater and, animal egg samples resulted in a total of 94 isolates. Species identification based on the internal transcribed spacer regions of the rDNA revealed that the yellow sediment samples had the highest species richness with 25 species, followed by the black sediment (23) and the crab (13). The Ascomycota was dominant over the Basidiomycota; the dominant orders were Agaricales, Capnodiales, Eurotiales, Hypocreales, Pleosporales, Polyporales and Xylariales. Hortaea werneckii was the only common fungus isolated from the crab, seawater, yellow and black sediment samples. The metabarcoding analysis amplifying a small fragment of the rDNA (from 18S to 5.8S) recovered 7–27 species from the black sediment and 12–27 species from the yellow sediment samples and all species belonged to the Ascomycota and the Basidiomycota. In the yellow sediments, the dominant order was Pleosporales and this order was also dominant in the black sediment together with Sporidiobolales. Based on the results from both methods, 54 and 49 species were found in the black and yellow sediments, respectively. Overall, a higher proportion of Ascomycota (~70%) over Basidiomycota was recovered in the yellow sediment and the two phyla were equally abundant in the black sediment. The top five dominant fungal orders in descending order based on species richness were Pleosporales>Eurotiales>Polyporales>Hypocreales>Capnodiales in the black sediment samples, and Polyporales>Pleosporales>Eurotiales>Capnodiales>Hypocreales in the yellow sediment samples. This study is the first to observe a high diversity of fungi associated with various substrates at a marine shallow water hydrothermal vent ecosystem. While some fungi found in this study were terrestrial species and their airborne spores might have been deposited into the marine sediment, several pathogenic fungi of animals, including Acremonium spp., Aspergillus spp., Fusarium spp., Malassezia spp., Hortaea werneckii, Parengyodontium album, and Westerdykella dispersa, were recovered suggesting that these fungi may be able to cause diseases of marine animals.
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spelling pubmed-69368832020-01-07 Insights into fungal diversity of a shallow-water hydrothermal vent field at Kueishan Island, Taiwan by culture-based and metabarcoding analyses Pang, Ka-Lai Guo, Sheng-Yu Chen, I-An Burgaud, Gäetan Luo, Zhu-Hua Dahms, Hans U. Hwang, Jiang-Shiou Lin, Yi-Li Huang, Jian-Shun Ho, Tsz-Wai Tsang, Ling-Ming Chiang, Michael Wai-Lun Cha, Hyo-Jung PLoS One Research Article This paper reports the diversity of fungi associated with substrates collected at a shallow hydrothermal vent field at Kueishan Island, Taiwan, using both culture-based and metabarcoding methods. Culture of fungi from yellow sediment (with visible sulfur granules), black sediment (no visible sulfur granules), the vent crab Xenograpsus testudinatus, seawater and, animal egg samples resulted in a total of 94 isolates. Species identification based on the internal transcribed spacer regions of the rDNA revealed that the yellow sediment samples had the highest species richness with 25 species, followed by the black sediment (23) and the crab (13). The Ascomycota was dominant over the Basidiomycota; the dominant orders were Agaricales, Capnodiales, Eurotiales, Hypocreales, Pleosporales, Polyporales and Xylariales. Hortaea werneckii was the only common fungus isolated from the crab, seawater, yellow and black sediment samples. The metabarcoding analysis amplifying a small fragment of the rDNA (from 18S to 5.8S) recovered 7–27 species from the black sediment and 12–27 species from the yellow sediment samples and all species belonged to the Ascomycota and the Basidiomycota. In the yellow sediments, the dominant order was Pleosporales and this order was also dominant in the black sediment together with Sporidiobolales. Based on the results from both methods, 54 and 49 species were found in the black and yellow sediments, respectively. Overall, a higher proportion of Ascomycota (~70%) over Basidiomycota was recovered in the yellow sediment and the two phyla were equally abundant in the black sediment. The top five dominant fungal orders in descending order based on species richness were Pleosporales>Eurotiales>Polyporales>Hypocreales>Capnodiales in the black sediment samples, and Polyporales>Pleosporales>Eurotiales>Capnodiales>Hypocreales in the yellow sediment samples. This study is the first to observe a high diversity of fungi associated with various substrates at a marine shallow water hydrothermal vent ecosystem. While some fungi found in this study were terrestrial species and their airborne spores might have been deposited into the marine sediment, several pathogenic fungi of animals, including Acremonium spp., Aspergillus spp., Fusarium spp., Malassezia spp., Hortaea werneckii, Parengyodontium album, and Westerdykella dispersa, were recovered suggesting that these fungi may be able to cause diseases of marine animals. Public Library of Science 2019-12-30 /pmc/articles/PMC6936883/ /pubmed/31887170 http://dx.doi.org/10.1371/journal.pone.0226616 Text en © 2019 Pang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Pang, Ka-Lai
Guo, Sheng-Yu
Chen, I-An
Burgaud, Gäetan
Luo, Zhu-Hua
Dahms, Hans U.
Hwang, Jiang-Shiou
Lin, Yi-Li
Huang, Jian-Shun
Ho, Tsz-Wai
Tsang, Ling-Ming
Chiang, Michael Wai-Lun
Cha, Hyo-Jung
Insights into fungal diversity of a shallow-water hydrothermal vent field at Kueishan Island, Taiwan by culture-based and metabarcoding analyses
title Insights into fungal diversity of a shallow-water hydrothermal vent field at Kueishan Island, Taiwan by culture-based and metabarcoding analyses
title_full Insights into fungal diversity of a shallow-water hydrothermal vent field at Kueishan Island, Taiwan by culture-based and metabarcoding analyses
title_fullStr Insights into fungal diversity of a shallow-water hydrothermal vent field at Kueishan Island, Taiwan by culture-based and metabarcoding analyses
title_full_unstemmed Insights into fungal diversity of a shallow-water hydrothermal vent field at Kueishan Island, Taiwan by culture-based and metabarcoding analyses
title_short Insights into fungal diversity of a shallow-water hydrothermal vent field at Kueishan Island, Taiwan by culture-based and metabarcoding analyses
title_sort insights into fungal diversity of a shallow-water hydrothermal vent field at kueishan island, taiwan by culture-based and metabarcoding analyses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6936883/
https://www.ncbi.nlm.nih.gov/pubmed/31887170
http://dx.doi.org/10.1371/journal.pone.0226616
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