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Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae)
BACKGROUND: Orange jasmine has a complex nomenclatural history and is now known as Murraya paniculata (L.) Jack. Our interest in this common ornamental stemmed from the need to resolve its identity and the identities of closely related taxa as hosts of the pathogen ‘Candidatus Liberibacter asiaticus...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6937641/ https://www.ncbi.nlm.nih.gov/pubmed/31888450 http://dx.doi.org/10.1186/s12862-019-1555-4 |
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author | Nguyen, Chung Huy Beattie, G. Andrew C. Haigh, Anthony M. Astuti, Inggit Puji Mabberley, David J. Weston, Peter H. Holford, Paul |
author_facet | Nguyen, Chung Huy Beattie, G. Andrew C. Haigh, Anthony M. Astuti, Inggit Puji Mabberley, David J. Weston, Peter H. Holford, Paul |
author_sort | Nguyen, Chung Huy |
collection | PubMed |
description | BACKGROUND: Orange jasmine has a complex nomenclatural history and is now known as Murraya paniculata (L.) Jack. Our interest in this common ornamental stemmed from the need to resolve its identity and the identities of closely related taxa as hosts of the pathogen ‘Candidatus Liberibacter asiaticus’ and its vector Diaphorina citri. Understanding these microbe-vector-plant relationships has been hampered by taxonomic confusion surrounding Murraya at both the generic and specific levels. RESULTS: To resolve the taxonomic uncertainty, six regions of the maternally-inherited chloroplastal genome and part of the nuclear-encoded ITS region were amplified from 85 accessions of Murraya and Merrillia using the polymerase chain reaction (PCR). Clustering used maximum parsimony (MP), maximum likelihood (ML) and Bayesian inference (BI). Chronograms were produced for molecular dating, and to test the monophyly of Murraya rigorously, using selected accessions of Murraya and 26 accessions of the Rutaceae and Simarubaceae. Sequence data from the ITS and chloroplastal regions suggest that Murraya paniculata (sensu (Swingle WT and Reece CR, The Citrus Industry, p. 190–430, 1967)) can be separated into four distinct but morphologically somewhat cryptic taxa: Murraya paniculata (sensu (Mabberley DJ, Taxon 65:366–371, 2016)), M. elongata, M. sumatrana and M. lucida. In addition, Murraya omphalocarpa was identified as a putative hybrid of M. paniculata and M. lucida with two geographically isolated nothovarieties representing reciprocal crosses. Murraya is monophyletic, and molecular dating suggests that it diverged from Merrillia during the Miocene (23–5 Ma) with this Murraya group speciating and dispersing during the Middle Miocene onwards. CONCLUSIONS: The accessions from Asia and Australasia used in this study grouped into biogeographical regions that match herbarium specimen records for the taxa that suggest natural allopatric distributions with limited overlap and hybridity. Murraya paniculata has been distributed around the world as an ornamental plant. The division of the Murraya paniculata complex into four species with a rare hybrid also confirms morphological studies. |
format | Online Article Text |
id | pubmed-6937641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69376412019-12-31 Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae) Nguyen, Chung Huy Beattie, G. Andrew C. Haigh, Anthony M. Astuti, Inggit Puji Mabberley, David J. Weston, Peter H. Holford, Paul BMC Evol Biol Research Article BACKGROUND: Orange jasmine has a complex nomenclatural history and is now known as Murraya paniculata (L.) Jack. Our interest in this common ornamental stemmed from the need to resolve its identity and the identities of closely related taxa as hosts of the pathogen ‘Candidatus Liberibacter asiaticus’ and its vector Diaphorina citri. Understanding these microbe-vector-plant relationships has been hampered by taxonomic confusion surrounding Murraya at both the generic and specific levels. RESULTS: To resolve the taxonomic uncertainty, six regions of the maternally-inherited chloroplastal genome and part of the nuclear-encoded ITS region were amplified from 85 accessions of Murraya and Merrillia using the polymerase chain reaction (PCR). Clustering used maximum parsimony (MP), maximum likelihood (ML) and Bayesian inference (BI). Chronograms were produced for molecular dating, and to test the monophyly of Murraya rigorously, using selected accessions of Murraya and 26 accessions of the Rutaceae and Simarubaceae. Sequence data from the ITS and chloroplastal regions suggest that Murraya paniculata (sensu (Swingle WT and Reece CR, The Citrus Industry, p. 190–430, 1967)) can be separated into four distinct but morphologically somewhat cryptic taxa: Murraya paniculata (sensu (Mabberley DJ, Taxon 65:366–371, 2016)), M. elongata, M. sumatrana and M. lucida. In addition, Murraya omphalocarpa was identified as a putative hybrid of M. paniculata and M. lucida with two geographically isolated nothovarieties representing reciprocal crosses. Murraya is monophyletic, and molecular dating suggests that it diverged from Merrillia during the Miocene (23–5 Ma) with this Murraya group speciating and dispersing during the Middle Miocene onwards. CONCLUSIONS: The accessions from Asia and Australasia used in this study grouped into biogeographical regions that match herbarium specimen records for the taxa that suggest natural allopatric distributions with limited overlap and hybridity. Murraya paniculata has been distributed around the world as an ornamental plant. The division of the Murraya paniculata complex into four species with a rare hybrid also confirms morphological studies. BioMed Central 2019-12-30 /pmc/articles/PMC6937641/ /pubmed/31888450 http://dx.doi.org/10.1186/s12862-019-1555-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Nguyen, Chung Huy Beattie, G. Andrew C. Haigh, Anthony M. Astuti, Inggit Puji Mabberley, David J. Weston, Peter H. Holford, Paul Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae) |
title | Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae) |
title_full | Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae) |
title_fullStr | Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae) |
title_full_unstemmed | Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae) |
title_short | Molecular differentiation of the Murraya paniculata Complex (Rutaceae: Aurantioideae: Aurantieae) |
title_sort | molecular differentiation of the murraya paniculata complex (rutaceae: aurantioideae: aurantieae) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6937641/ https://www.ncbi.nlm.nih.gov/pubmed/31888450 http://dx.doi.org/10.1186/s12862-019-1555-4 |
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