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One-shot phase-recovery using a cellphone RGB camera on a Jamin-Lebedeff microscope
Jamin-Lebedeff (JL) polarization interference microscopy is a classical method for determining the change in the optical path of transparent tissues. Whilst a differential interference contrast (DIC) microscopy interferes an image with itself shifted by half a point spread function, the shear betwee...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6938357/ https://www.ncbi.nlm.nih.gov/pubmed/31891618 http://dx.doi.org/10.1371/journal.pone.0227096 |
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author | Diederich, Benedict Marsikova, Barbora Amos, Brad Heintzmann, Rainer |
author_facet | Diederich, Benedict Marsikova, Barbora Amos, Brad Heintzmann, Rainer |
author_sort | Diederich, Benedict |
collection | PubMed |
description | Jamin-Lebedeff (JL) polarization interference microscopy is a classical method for determining the change in the optical path of transparent tissues. Whilst a differential interference contrast (DIC) microscopy interferes an image with itself shifted by half a point spread function, the shear between the object and reference image in a JL-microscope is about half the field of view. The optical path difference (OPD) between the sample and reference region (assumed to be empty) is encoded into a color by white-light interference. From a color-table, the Michel-Levy chart, the OPD can be deduced. In cytology JL-imaging can be used as a way to determine the OPD which closely corresponds to the dry mass per area of cells in a single image. Like in other interference microscopy methods (e.g. holography), we present a phase retrieval method relying on single-shot measurements only, thus allowing real-time quantitative phase measurements. This is achieved by adding several customized 3D-printed parts (e.g. rotational polarization-filter holders) and a modern cellphone with an RGB-camera to the Jamin-Lebedeff setup, thus bringing an old microscope back to life. The algorithm is calibrated using a reference image of a known phase object (e.g. optical fiber). A gradient-descent based inverse problem generates an inverse look-up-table (LUT) which is used to convert the measured RGB signal of a phase-sample into an OPD. To account for possible ambiguities in the phase-map or phase-unwrapping artifacts we introduce a total-variation based regularization. We present results from fixed and living biological samples as well as reference samples for comparison. |
format | Online Article Text |
id | pubmed-6938357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-69383572020-01-07 One-shot phase-recovery using a cellphone RGB camera on a Jamin-Lebedeff microscope Diederich, Benedict Marsikova, Barbora Amos, Brad Heintzmann, Rainer PLoS One Research Article Jamin-Lebedeff (JL) polarization interference microscopy is a classical method for determining the change in the optical path of transparent tissues. Whilst a differential interference contrast (DIC) microscopy interferes an image with itself shifted by half a point spread function, the shear between the object and reference image in a JL-microscope is about half the field of view. The optical path difference (OPD) between the sample and reference region (assumed to be empty) is encoded into a color by white-light interference. From a color-table, the Michel-Levy chart, the OPD can be deduced. In cytology JL-imaging can be used as a way to determine the OPD which closely corresponds to the dry mass per area of cells in a single image. Like in other interference microscopy methods (e.g. holography), we present a phase retrieval method relying on single-shot measurements only, thus allowing real-time quantitative phase measurements. This is achieved by adding several customized 3D-printed parts (e.g. rotational polarization-filter holders) and a modern cellphone with an RGB-camera to the Jamin-Lebedeff setup, thus bringing an old microscope back to life. The algorithm is calibrated using a reference image of a known phase object (e.g. optical fiber). A gradient-descent based inverse problem generates an inverse look-up-table (LUT) which is used to convert the measured RGB signal of a phase-sample into an OPD. To account for possible ambiguities in the phase-map or phase-unwrapping artifacts we introduce a total-variation based regularization. We present results from fixed and living biological samples as well as reference samples for comparison. Public Library of Science 2019-12-31 /pmc/articles/PMC6938357/ /pubmed/31891618 http://dx.doi.org/10.1371/journal.pone.0227096 Text en © 2019 Diederich et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Diederich, Benedict Marsikova, Barbora Amos, Brad Heintzmann, Rainer One-shot phase-recovery using a cellphone RGB camera on a Jamin-Lebedeff microscope |
title | One-shot phase-recovery using a cellphone RGB camera on a Jamin-Lebedeff microscope |
title_full | One-shot phase-recovery using a cellphone RGB camera on a Jamin-Lebedeff microscope |
title_fullStr | One-shot phase-recovery using a cellphone RGB camera on a Jamin-Lebedeff microscope |
title_full_unstemmed | One-shot phase-recovery using a cellphone RGB camera on a Jamin-Lebedeff microscope |
title_short | One-shot phase-recovery using a cellphone RGB camera on a Jamin-Lebedeff microscope |
title_sort | one-shot phase-recovery using a cellphone rgb camera on a jamin-lebedeff microscope |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6938357/ https://www.ncbi.nlm.nih.gov/pubmed/31891618 http://dx.doi.org/10.1371/journal.pone.0227096 |
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