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Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco

The present study was conducted in order to isolate, identify and characterize fowl aviadenovirus associated with inclusion body hepatitis (IBH) in three poultry farms (two of broiler chickens and one of breeder broiler chickens) in Morocco during 2015. Liver samples collected from affected three po...

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Autores principales: Abghour, Samira, Zro, Khalil, Mouahid, Mohammed, Tahiri, Fatima, Tarta, Meriam, Berrada, Jaouad, Kichou, Faouzi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6938405/
https://www.ncbi.nlm.nih.gov/pubmed/31891942
http://dx.doi.org/10.1371/journal.pone.0227004
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author Abghour, Samira
Zro, Khalil
Mouahid, Mohammed
Tahiri, Fatima
Tarta, Meriam
Berrada, Jaouad
Kichou, Faouzi
author_facet Abghour, Samira
Zro, Khalil
Mouahid, Mohammed
Tahiri, Fatima
Tarta, Meriam
Berrada, Jaouad
Kichou, Faouzi
author_sort Abghour, Samira
collection PubMed
description The present study was conducted in order to isolate, identify and characterize fowl aviadenovirus associated with inclusion body hepatitis (IBH) in three poultry farms (two of broiler chickens and one of breeder broiler chickens) in Morocco during 2015. Liver samples collected from affected three poultry farms were examined by histopathological examination. Tissue samples showing necrosis of hepatocytes associated with basophilic intranuclear inclusion bodies were homogenized and submitted to FAdV isolation in chicken embryo fibroblast (CEF) cell cultures and in SPF embryonated eggs. The cytopathic effect (CPE) was observed in the second passage with swelling and rounding of infected cells. The inoculated embryos were hemorrhagic and showed hepatitis with the presence of basophilic intra-nuclear inclusion bodies within hepatocytes. The presence of the virus was confirmed by conventional polymerase chain reaction based on hexon gene from all investigated samples. Moreover, phylogenetic analysis of the hexon gene revealed that FAdVs isolated from different affected poultry belonged to FAdV 11 serotype of the D genotype group. This work is the first isolation in cell culture and SPF embryonated eggs of FAdV from Moroccan broilers and breeder broiler chickens with IBH.
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spelling pubmed-69384052020-01-07 Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco Abghour, Samira Zro, Khalil Mouahid, Mohammed Tahiri, Fatima Tarta, Meriam Berrada, Jaouad Kichou, Faouzi PLoS One Research Article The present study was conducted in order to isolate, identify and characterize fowl aviadenovirus associated with inclusion body hepatitis (IBH) in three poultry farms (two of broiler chickens and one of breeder broiler chickens) in Morocco during 2015. Liver samples collected from affected three poultry farms were examined by histopathological examination. Tissue samples showing necrosis of hepatocytes associated with basophilic intranuclear inclusion bodies were homogenized and submitted to FAdV isolation in chicken embryo fibroblast (CEF) cell cultures and in SPF embryonated eggs. The cytopathic effect (CPE) was observed in the second passage with swelling and rounding of infected cells. The inoculated embryos were hemorrhagic and showed hepatitis with the presence of basophilic intra-nuclear inclusion bodies within hepatocytes. The presence of the virus was confirmed by conventional polymerase chain reaction based on hexon gene from all investigated samples. Moreover, phylogenetic analysis of the hexon gene revealed that FAdVs isolated from different affected poultry belonged to FAdV 11 serotype of the D genotype group. This work is the first isolation in cell culture and SPF embryonated eggs of FAdV from Moroccan broilers and breeder broiler chickens with IBH. Public Library of Science 2019-12-31 /pmc/articles/PMC6938405/ /pubmed/31891942 http://dx.doi.org/10.1371/journal.pone.0227004 Text en © 2019 Abghour et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Abghour, Samira
Zro, Khalil
Mouahid, Mohammed
Tahiri, Fatima
Tarta, Meriam
Berrada, Jaouad
Kichou, Faouzi
Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco
title Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco
title_full Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco
title_fullStr Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco
title_full_unstemmed Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco
title_short Isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in Morocco
title_sort isolation and characterization of fowl aviadenovirus serotype 11 from chickens with inclusion body hepatitis in morocco
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6938405/
https://www.ncbi.nlm.nih.gov/pubmed/31891942
http://dx.doi.org/10.1371/journal.pone.0227004
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