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Intraoperative assessment of skull base tumors using stimulated Raman scattering microscopy

Intraoperative consultations, used to guide tumor resection, can present histopathological findings that are challenging to interpret due to artefacts from tissue cryosectioning and conventional staining. Stimulated Raman histology (SRH), a label-free imaging technique for unprocessed biospecimens,...

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Autores principales: Shin, Kseniya S., Francis, Andrew T., Hill, Andrew H., Laohajaratsang, Mint, Cimino, Patrick J., Latimer, Caitlin S., Gonzalez-Cuyar, Luis F., Sekhar, Laligam N., Juric-Sekhar, Gordana, Fu, Dan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6938502/
https://www.ncbi.nlm.nih.gov/pubmed/31892723
http://dx.doi.org/10.1038/s41598-019-56932-8
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author Shin, Kseniya S.
Francis, Andrew T.
Hill, Andrew H.
Laohajaratsang, Mint
Cimino, Patrick J.
Latimer, Caitlin S.
Gonzalez-Cuyar, Luis F.
Sekhar, Laligam N.
Juric-Sekhar, Gordana
Fu, Dan
author_facet Shin, Kseniya S.
Francis, Andrew T.
Hill, Andrew H.
Laohajaratsang, Mint
Cimino, Patrick J.
Latimer, Caitlin S.
Gonzalez-Cuyar, Luis F.
Sekhar, Laligam N.
Juric-Sekhar, Gordana
Fu, Dan
author_sort Shin, Kseniya S.
collection PubMed
description Intraoperative consultations, used to guide tumor resection, can present histopathological findings that are challenging to interpret due to artefacts from tissue cryosectioning and conventional staining. Stimulated Raman histology (SRH), a label-free imaging technique for unprocessed biospecimens, has demonstrated promise in a limited subset of tumors. Here, we target unexplored skull base tumors using a fast simultaneous two-channel stimulated Raman scattering (SRS) imaging technique and a new pseudo-hematoxylin and eosin (H&E) recoloring methodology. To quantitatively evaluate the efficacy of our approach, we use modularized assessment of diagnostic accuracy beyond cancer/non-cancer determination and neuropathologist confidence for SRH images contrasted to H&E-stained frozen and formalin-fixed paraffin-embedded (FFPE) tissue sections. Our results reveal that SRH is effective for establishing a diagnosis using fresh tissue in most cases with 87% accuracy relative to H&E-stained FFPE sections. Further analysis of discrepant case interpretation suggests that pseudo-H&E recoloring underutilizes the rich chemical information offered by SRS imaging, and an improved diagnosis can be achieved if full SRS information is used. In summary, our findings show that pseudo-H&E recolored SRS images in combination with lipid and protein chemical information can maximize the use of SRS during intraoperative pathologic consultation with implications for tissue preservation and augmented diagnostic utility.
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spelling pubmed-69385022020-01-06 Intraoperative assessment of skull base tumors using stimulated Raman scattering microscopy Shin, Kseniya S. Francis, Andrew T. Hill, Andrew H. Laohajaratsang, Mint Cimino, Patrick J. Latimer, Caitlin S. Gonzalez-Cuyar, Luis F. Sekhar, Laligam N. Juric-Sekhar, Gordana Fu, Dan Sci Rep Article Intraoperative consultations, used to guide tumor resection, can present histopathological findings that are challenging to interpret due to artefacts from tissue cryosectioning and conventional staining. Stimulated Raman histology (SRH), a label-free imaging technique for unprocessed biospecimens, has demonstrated promise in a limited subset of tumors. Here, we target unexplored skull base tumors using a fast simultaneous two-channel stimulated Raman scattering (SRS) imaging technique and a new pseudo-hematoxylin and eosin (H&E) recoloring methodology. To quantitatively evaluate the efficacy of our approach, we use modularized assessment of diagnostic accuracy beyond cancer/non-cancer determination and neuropathologist confidence for SRH images contrasted to H&E-stained frozen and formalin-fixed paraffin-embedded (FFPE) tissue sections. Our results reveal that SRH is effective for establishing a diagnosis using fresh tissue in most cases with 87% accuracy relative to H&E-stained FFPE sections. Further analysis of discrepant case interpretation suggests that pseudo-H&E recoloring underutilizes the rich chemical information offered by SRS imaging, and an improved diagnosis can be achieved if full SRS information is used. In summary, our findings show that pseudo-H&E recolored SRS images in combination with lipid and protein chemical information can maximize the use of SRS during intraoperative pathologic consultation with implications for tissue preservation and augmented diagnostic utility. Nature Publishing Group UK 2019-12-31 /pmc/articles/PMC6938502/ /pubmed/31892723 http://dx.doi.org/10.1038/s41598-019-56932-8 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Shin, Kseniya S.
Francis, Andrew T.
Hill, Andrew H.
Laohajaratsang, Mint
Cimino, Patrick J.
Latimer, Caitlin S.
Gonzalez-Cuyar, Luis F.
Sekhar, Laligam N.
Juric-Sekhar, Gordana
Fu, Dan
Intraoperative assessment of skull base tumors using stimulated Raman scattering microscopy
title Intraoperative assessment of skull base tumors using stimulated Raman scattering microscopy
title_full Intraoperative assessment of skull base tumors using stimulated Raman scattering microscopy
title_fullStr Intraoperative assessment of skull base tumors using stimulated Raman scattering microscopy
title_full_unstemmed Intraoperative assessment of skull base tumors using stimulated Raman scattering microscopy
title_short Intraoperative assessment of skull base tumors using stimulated Raman scattering microscopy
title_sort intraoperative assessment of skull base tumors using stimulated raman scattering microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6938502/
https://www.ncbi.nlm.nih.gov/pubmed/31892723
http://dx.doi.org/10.1038/s41598-019-56932-8
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