Cargando…

Strengthening antioxidant defense & cardio protection by Piper betle: An in-vitro study

INTRODUCTION: The purpose of this research work was to evaluate Piper betle ethyl acetate extract (PBEA) for its free radical scavenging, antioxidant, anti-apoptotic activities and its role in protecting against oxidative cardiac cell injury. METHODS: The Free radical scavenging activity and antioxi...

Descripción completa

Detalles Bibliográficos
Autores principales: Savsani, Hardik, Srivastava, Abhay, Gupta, Sarita, Patel, Kirti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6939052/
https://www.ncbi.nlm.nih.gov/pubmed/31909246
http://dx.doi.org/10.1016/j.heliyon.2019.e03041
_version_ 1783484159177523200
author Savsani, Hardik
Srivastava, Abhay
Gupta, Sarita
Patel, Kirti
author_facet Savsani, Hardik
Srivastava, Abhay
Gupta, Sarita
Patel, Kirti
author_sort Savsani, Hardik
collection PubMed
description INTRODUCTION: The purpose of this research work was to evaluate Piper betle ethyl acetate extract (PBEA) for its free radical scavenging, antioxidant, anti-apoptotic activities and its role in protecting against oxidative cardiac cell injury. METHODS: The Free radical scavenging activity and antioxidant potential of PBEA were evaluated using various non-cellular methods (1,1-Diphenyl-2-picrylhydrazyl, β-carotene bleaching, superoxide anion, hydroxyl radical, hydrogen peroxide(,) Reducing power, Total phenolics and Total flavonoids). PBEA was standardized with Eugenol by GC-FID analysis. Furthermore, PBEA was also assessed for its cytotoprotective effect against 100μM H(2)O(2) in H9c2 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Intracellular reactive oxygen species scavenging and anti-apoptoic activity of PBEA was assessed by using 2′, 7′-Dichlorofluorescein diacetate and Annexin- Propidium Iodide, respectively. RESULTS: PBEA exhibited radical scavenging and antioxidant defense response at different magnitudes of potency. Eugenol, a cardiac protective bioactive molecule in PBEA was found to be 43.43 ± 1.46 mg/g of PBEA extract. Further, pre-incubation of H9c2 cells with 10 μg/ml PBEA for 24 h exhibited remarkable cytoprotective effect against H(2)O(2) induced oxidative stress. PBEA at 10 μg/ml dose with 24 h contact with H9c2 cells significantly enhanced the activity of cellular defense system and significantly decreased intracellular ROS (P < 0.001) and apoptosis (P < 0.01) thereby protecting against the cytotoxic effects of H(2)O(2). CONCLUSION: These outcomes indicated that PBEA could shield against oxidative and apoptotic cardiac cell injury in invitro studies. Thus, PBEA might be a desirable antioxidant of natural origin that has future clinical implications in both health care and food industry.
format Online
Article
Text
id pubmed-6939052
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-69390522020-01-06 Strengthening antioxidant defense & cardio protection by Piper betle: An in-vitro study Savsani, Hardik Srivastava, Abhay Gupta, Sarita Patel, Kirti Heliyon Article INTRODUCTION: The purpose of this research work was to evaluate Piper betle ethyl acetate extract (PBEA) for its free radical scavenging, antioxidant, anti-apoptotic activities and its role in protecting against oxidative cardiac cell injury. METHODS: The Free radical scavenging activity and antioxidant potential of PBEA were evaluated using various non-cellular methods (1,1-Diphenyl-2-picrylhydrazyl, β-carotene bleaching, superoxide anion, hydroxyl radical, hydrogen peroxide(,) Reducing power, Total phenolics and Total flavonoids). PBEA was standardized with Eugenol by GC-FID analysis. Furthermore, PBEA was also assessed for its cytotoprotective effect against 100μM H(2)O(2) in H9c2 cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Intracellular reactive oxygen species scavenging and anti-apoptoic activity of PBEA was assessed by using 2′, 7′-Dichlorofluorescein diacetate and Annexin- Propidium Iodide, respectively. RESULTS: PBEA exhibited radical scavenging and antioxidant defense response at different magnitudes of potency. Eugenol, a cardiac protective bioactive molecule in PBEA was found to be 43.43 ± 1.46 mg/g of PBEA extract. Further, pre-incubation of H9c2 cells with 10 μg/ml PBEA for 24 h exhibited remarkable cytoprotective effect against H(2)O(2) induced oxidative stress. PBEA at 10 μg/ml dose with 24 h contact with H9c2 cells significantly enhanced the activity of cellular defense system and significantly decreased intracellular ROS (P < 0.001) and apoptosis (P < 0.01) thereby protecting against the cytotoxic effects of H(2)O(2). CONCLUSION: These outcomes indicated that PBEA could shield against oxidative and apoptotic cardiac cell injury in invitro studies. Thus, PBEA might be a desirable antioxidant of natural origin that has future clinical implications in both health care and food industry. Elsevier 2019-12-26 /pmc/articles/PMC6939052/ /pubmed/31909246 http://dx.doi.org/10.1016/j.heliyon.2019.e03041 Text en © 2019 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Savsani, Hardik
Srivastava, Abhay
Gupta, Sarita
Patel, Kirti
Strengthening antioxidant defense & cardio protection by Piper betle: An in-vitro study
title Strengthening antioxidant defense & cardio protection by Piper betle: An in-vitro study
title_full Strengthening antioxidant defense & cardio protection by Piper betle: An in-vitro study
title_fullStr Strengthening antioxidant defense & cardio protection by Piper betle: An in-vitro study
title_full_unstemmed Strengthening antioxidant defense & cardio protection by Piper betle: An in-vitro study
title_short Strengthening antioxidant defense & cardio protection by Piper betle: An in-vitro study
title_sort strengthening antioxidant defense & cardio protection by piper betle: an in-vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6939052/
https://www.ncbi.nlm.nih.gov/pubmed/31909246
http://dx.doi.org/10.1016/j.heliyon.2019.e03041
work_keys_str_mv AT savsanihardik strengtheningantioxidantdefensecardioprotectionbypiperbetleaninvitrostudy
AT srivastavaabhay strengtheningantioxidantdefensecardioprotectionbypiperbetleaninvitrostudy
AT guptasarita strengtheningantioxidantdefensecardioprotectionbypiperbetleaninvitrostudy
AT patelkirti strengtheningantioxidantdefensecardioprotectionbypiperbetleaninvitrostudy