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How can schistosome circulating antigen assays be best applied for diagnosing male genital schistosomiasis (MGS): an appraisal using exemplar MGS cases from a longitudinal cohort study among fishermen on the south shoreline of Lake Malawi
We provide an update on diagnostic methods for the detection of urogenital schistosomiasis (UGS) in men and highlight that satisfactory urine-antigen diagnostics for UGS lag much behind that for intestinal schistosomiasis, where application of a urine-based point-of-care strip assay, the circulating...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cambridge University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6939168/ https://www.ncbi.nlm.nih.gov/pubmed/31452477 http://dx.doi.org/10.1017/S0031182019000969 |
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author | Kayuni, S. A. Corstjens, P. L. A. M. LaCourse, E. J. Bartlett, K. E. Fawcett, J. Shaw, A. Makaula, P. Lampiao, F. Juziwelo, L. de Dood, C. J. Hoekstra, P. T. Verweij, J. J. Leutscher, P. D. C. van Dam, G. J. van Lieshout, L. Stothard, J. R. |
author_facet | Kayuni, S. A. Corstjens, P. L. A. M. LaCourse, E. J. Bartlett, K. E. Fawcett, J. Shaw, A. Makaula, P. Lampiao, F. Juziwelo, L. de Dood, C. J. Hoekstra, P. T. Verweij, J. J. Leutscher, P. D. C. van Dam, G. J. van Lieshout, L. Stothard, J. R. |
author_sort | Kayuni, S. A. |
collection | PubMed |
description | We provide an update on diagnostic methods for the detection of urogenital schistosomiasis (UGS) in men and highlight that satisfactory urine-antigen diagnostics for UGS lag much behind that for intestinal schistosomiasis, where application of a urine-based point-of-care strip assay, the circulating cathodic antigen (CCA) test, is now advocated. Making specific reference to male genital schistosomiasis (MGS), we place greater emphasis on parasitological detection methods and clinical assessment of internal genitalia with ultrasonography. Unlike the advances made in defining a clinical standard protocol for female genital schistosomiasis, MGS remains inadequately defined. Whilst urine filtration with microscopic examination for ova of Schistosoma haematobium is a convenient but error-prone proxy of MGS, we describe a novel low-cost sampling and direct visualization method for the enumeration of ova in semen. Using exemplar clinical cases of MGS from our longitudinal cohort study among fishermen along the shoreline of Lake Malawi, the portfolio of diagnostic needs is appraised including: the use of symptomatology questionnaires, urine analysis (egg count and CCA measurement), semen analysis (egg count, circulating anodic antigen measurement and real-time polymerase chain reaction analysis) alongside clinical assessment with portable ultrasonography. |
format | Online Article Text |
id | pubmed-6939168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Cambridge University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-69391682020-01-07 How can schistosome circulating antigen assays be best applied for diagnosing male genital schistosomiasis (MGS): an appraisal using exemplar MGS cases from a longitudinal cohort study among fishermen on the south shoreline of Lake Malawi Kayuni, S. A. Corstjens, P. L. A. M. LaCourse, E. J. Bartlett, K. E. Fawcett, J. Shaw, A. Makaula, P. Lampiao, F. Juziwelo, L. de Dood, C. J. Hoekstra, P. T. Verweij, J. J. Leutscher, P. D. C. van Dam, G. J. van Lieshout, L. Stothard, J. R. Parasitology Research Article We provide an update on diagnostic methods for the detection of urogenital schistosomiasis (UGS) in men and highlight that satisfactory urine-antigen diagnostics for UGS lag much behind that for intestinal schistosomiasis, where application of a urine-based point-of-care strip assay, the circulating cathodic antigen (CCA) test, is now advocated. Making specific reference to male genital schistosomiasis (MGS), we place greater emphasis on parasitological detection methods and clinical assessment of internal genitalia with ultrasonography. Unlike the advances made in defining a clinical standard protocol for female genital schistosomiasis, MGS remains inadequately defined. Whilst urine filtration with microscopic examination for ova of Schistosoma haematobium is a convenient but error-prone proxy of MGS, we describe a novel low-cost sampling and direct visualization method for the enumeration of ova in semen. Using exemplar clinical cases of MGS from our longitudinal cohort study among fishermen along the shoreline of Lake Malawi, the portfolio of diagnostic needs is appraised including: the use of symptomatology questionnaires, urine analysis (egg count and CCA measurement), semen analysis (egg count, circulating anodic antigen measurement and real-time polymerase chain reaction analysis) alongside clinical assessment with portable ultrasonography. Cambridge University Press 2019-12 2019-09-23 /pmc/articles/PMC6939168/ /pubmed/31452477 http://dx.doi.org/10.1017/S0031182019000969 Text en © Cambridge University Press 2019 http://creativecommons.org/licenses/by/4.0/ This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Kayuni, S. A. Corstjens, P. L. A. M. LaCourse, E. J. Bartlett, K. E. Fawcett, J. Shaw, A. Makaula, P. Lampiao, F. Juziwelo, L. de Dood, C. J. Hoekstra, P. T. Verweij, J. J. Leutscher, P. D. C. van Dam, G. J. van Lieshout, L. Stothard, J. R. How can schistosome circulating antigen assays be best applied for diagnosing male genital schistosomiasis (MGS): an appraisal using exemplar MGS cases from a longitudinal cohort study among fishermen on the south shoreline of Lake Malawi |
title | How can schistosome circulating antigen assays be best applied for diagnosing male genital schistosomiasis (MGS): an appraisal using exemplar MGS cases from a longitudinal cohort study among fishermen on the south shoreline of Lake Malawi |
title_full | How can schistosome circulating antigen assays be best applied for diagnosing male genital schistosomiasis (MGS): an appraisal using exemplar MGS cases from a longitudinal cohort study among fishermen on the south shoreline of Lake Malawi |
title_fullStr | How can schistosome circulating antigen assays be best applied for diagnosing male genital schistosomiasis (MGS): an appraisal using exemplar MGS cases from a longitudinal cohort study among fishermen on the south shoreline of Lake Malawi |
title_full_unstemmed | How can schistosome circulating antigen assays be best applied for diagnosing male genital schistosomiasis (MGS): an appraisal using exemplar MGS cases from a longitudinal cohort study among fishermen on the south shoreline of Lake Malawi |
title_short | How can schistosome circulating antigen assays be best applied for diagnosing male genital schistosomiasis (MGS): an appraisal using exemplar MGS cases from a longitudinal cohort study among fishermen on the south shoreline of Lake Malawi |
title_sort | how can schistosome circulating antigen assays be best applied for diagnosing male genital schistosomiasis (mgs): an appraisal using exemplar mgs cases from a longitudinal cohort study among fishermen on the south shoreline of lake malawi |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6939168/ https://www.ncbi.nlm.nih.gov/pubmed/31452477 http://dx.doi.org/10.1017/S0031182019000969 |
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