A simple enzymatic assay for the quantification of C1-specific cellulose oxidation by lytic polysaccharide monooxygenases

OBJECTIVE: The development of an enzymatic assay for the specific quantification of the C1-oxidation product, i.e. gluconic acid of cellulose active lytic polysaccharide monooxygenases (LPMOs). RESULTS: In combination with a β-glucosidase, the spectrophotometrical assay can reliably quantify the spe...

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Detalles Bibliográficos
Autores principales: Keller, M. B., Felby, C., Labate, C. A., Pellegrini, V. O. A., Higasi, P., Singh, R. K., Polikarpov, I., Blossom, B. M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2019
Materias:
Original Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940319/
https://www.ncbi.nlm.nih.gov/pubmed/31745843
http://dx.doi.org/10.1007/s10529-019-02760-9

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