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Measuring local-directional resolution and local anisotropy in cryo-EM maps

The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic...

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Detalles Bibliográficos
Autores principales: Vilas, Jose Luis, Tagare, Hemant D., Vargas, Javier, Carazo, Jose Maria, Sorzano, Carlos Oscar S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940361/
https://www.ncbi.nlm.nih.gov/pubmed/31896756
http://dx.doi.org/10.1038/s41467-019-13742-w
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author Vilas, Jose Luis
Tagare, Hemant D.
Vargas, Javier
Carazo, Jose Maria
Sorzano, Carlos Oscar S.
author_facet Vilas, Jose Luis
Tagare, Hemant D.
Vargas, Javier
Carazo, Jose Maria
Sorzano, Carlos Oscar S.
author_sort Vilas, Jose Luis
collection PubMed
description The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation.
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spelling pubmed-69403612020-01-06 Measuring local-directional resolution and local anisotropy in cryo-EM maps Vilas, Jose Luis Tagare, Hemant D. Vargas, Javier Carazo, Jose Maria Sorzano, Carlos Oscar S. Nat Commun Article The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation. Nature Publishing Group UK 2020-01-02 /pmc/articles/PMC6940361/ /pubmed/31896756 http://dx.doi.org/10.1038/s41467-019-13742-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Vilas, Jose Luis
Tagare, Hemant D.
Vargas, Javier
Carazo, Jose Maria
Sorzano, Carlos Oscar S.
Measuring local-directional resolution and local anisotropy in cryo-EM maps
title Measuring local-directional resolution and local anisotropy in cryo-EM maps
title_full Measuring local-directional resolution and local anisotropy in cryo-EM maps
title_fullStr Measuring local-directional resolution and local anisotropy in cryo-EM maps
title_full_unstemmed Measuring local-directional resolution and local anisotropy in cryo-EM maps
title_short Measuring local-directional resolution and local anisotropy in cryo-EM maps
title_sort measuring local-directional resolution and local anisotropy in cryo-em maps
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940361/
https://www.ncbi.nlm.nih.gov/pubmed/31896756
http://dx.doi.org/10.1038/s41467-019-13742-w
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