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Measuring local-directional resolution and local anisotropy in cryo-EM maps
The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940361/ https://www.ncbi.nlm.nih.gov/pubmed/31896756 http://dx.doi.org/10.1038/s41467-019-13742-w |
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author | Vilas, Jose Luis Tagare, Hemant D. Vargas, Javier Carazo, Jose Maria Sorzano, Carlos Oscar S. |
author_facet | Vilas, Jose Luis Tagare, Hemant D. Vargas, Javier Carazo, Jose Maria Sorzano, Carlos Oscar S. |
author_sort | Vilas, Jose Luis |
collection | PubMed |
description | The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation. |
format | Online Article Text |
id | pubmed-6940361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69403612020-01-06 Measuring local-directional resolution and local anisotropy in cryo-EM maps Vilas, Jose Luis Tagare, Hemant D. Vargas, Javier Carazo, Jose Maria Sorzano, Carlos Oscar S. Nat Commun Article The introduction of local resolution has enormously helped the understanding of cryo-EM maps. Still, for any given pixel it is a global, aggregated value, that makes impossible the individual analysis of the contribution of the different projection directions. We introduce MonoDir, a fully automatic, parameter-free method that, starting only from the final cryo-EM map, decomposes local resolution into the different projection directions, providing a detailed level of analysis of the final map. Many applications of directional local resolution are possible, and we concentrate here on map quality and validation. Nature Publishing Group UK 2020-01-02 /pmc/articles/PMC6940361/ /pubmed/31896756 http://dx.doi.org/10.1038/s41467-019-13742-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Vilas, Jose Luis Tagare, Hemant D. Vargas, Javier Carazo, Jose Maria Sorzano, Carlos Oscar S. Measuring local-directional resolution and local anisotropy in cryo-EM maps |
title | Measuring local-directional resolution and local anisotropy in cryo-EM maps |
title_full | Measuring local-directional resolution and local anisotropy in cryo-EM maps |
title_fullStr | Measuring local-directional resolution and local anisotropy in cryo-EM maps |
title_full_unstemmed | Measuring local-directional resolution and local anisotropy in cryo-EM maps |
title_short | Measuring local-directional resolution and local anisotropy in cryo-EM maps |
title_sort | measuring local-directional resolution and local anisotropy in cryo-em maps |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940361/ https://www.ncbi.nlm.nih.gov/pubmed/31896756 http://dx.doi.org/10.1038/s41467-019-13742-w |
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