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LYVE1 Marks the Divergence of Yolk Sac Definitive Hemogenic Endothelium from the Primitive Erythroid Lineage

The contribution of the different waves and sites of developmental hematopoiesis to fetal and adult blood production remains unclear. Here, we identify lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) as a marker of yolk sac (YS) endothelium and definitive hematopoietic stem and progenitor...

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Autores principales: Lee, Lydia K., Ghorbanian, Yasamine, Wang, Wenyuan, Wang, Yanling, Kim, Yeon Joo, Weissman, Irving L., Inlay, Matthew A., Mikkola, Hanna K.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940422/
https://www.ncbi.nlm.nih.gov/pubmed/27880904
http://dx.doi.org/10.1016/j.celrep.2016.10.080
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author Lee, Lydia K.
Ghorbanian, Yasamine
Wang, Wenyuan
Wang, Yanling
Kim, Yeon Joo
Weissman, Irving L.
Inlay, Matthew A.
Mikkola, Hanna K.A.
author_facet Lee, Lydia K.
Ghorbanian, Yasamine
Wang, Wenyuan
Wang, Yanling
Kim, Yeon Joo
Weissman, Irving L.
Inlay, Matthew A.
Mikkola, Hanna K.A.
author_sort Lee, Lydia K.
collection PubMed
description The contribution of the different waves and sites of developmental hematopoiesis to fetal and adult blood production remains unclear. Here, we identify lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) as a marker of yolk sac (YS) endothelium and definitive hematopoietic stem and progenitor cells (HSPCs). Endothelium in mid-gestation YS and vitelline vessels, but not the dorsal aorta and placenta, were labeled by Lyve1-Cre. Most YSHSPCs and erythro-myeloid progenitors were Lyve1-Cre lineage traced, but primitive erythroid cells were not, suggesting that they represent distinct lineages. Fetal liver (FL) and adult HSPCs showed 35%–40% Lyve1-Cre marking. Analysis of circulation-deficient Ncx1(−/−) concepti identified the YS as a major source of Lyve1-Cre labeled HSPCs. FL proerythroblast marking was extensive at embryonic day (E) 11.5–13.5, but decreased to hematopoietic stem cell (HSC) levels by E16.5, suggesting that HSCs from multiple sources became responsible for erythropoiesis. Lyve1-Cre thus marks the divergence between YS primitive and definitive hematopoiesis and provides a tool for targeting YS definitive hematopoiesis and FL colonization.
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spelling pubmed-69404222020-01-03 LYVE1 Marks the Divergence of Yolk Sac Definitive Hemogenic Endothelium from the Primitive Erythroid Lineage Lee, Lydia K. Ghorbanian, Yasamine Wang, Wenyuan Wang, Yanling Kim, Yeon Joo Weissman, Irving L. Inlay, Matthew A. Mikkola, Hanna K.A. Cell Rep Article The contribution of the different waves and sites of developmental hematopoiesis to fetal and adult blood production remains unclear. Here, we identify lymphatic vessel endothelial hyaluronan receptor-1 (LYVE1) as a marker of yolk sac (YS) endothelium and definitive hematopoietic stem and progenitor cells (HSPCs). Endothelium in mid-gestation YS and vitelline vessels, but not the dorsal aorta and placenta, were labeled by Lyve1-Cre. Most YSHSPCs and erythro-myeloid progenitors were Lyve1-Cre lineage traced, but primitive erythroid cells were not, suggesting that they represent distinct lineages. Fetal liver (FL) and adult HSPCs showed 35%–40% Lyve1-Cre marking. Analysis of circulation-deficient Ncx1(−/−) concepti identified the YS as a major source of Lyve1-Cre labeled HSPCs. FL proerythroblast marking was extensive at embryonic day (E) 11.5–13.5, but decreased to hematopoietic stem cell (HSC) levels by E16.5, suggesting that HSCs from multiple sources became responsible for erythropoiesis. Lyve1-Cre thus marks the divergence between YS primitive and definitive hematopoiesis and provides a tool for targeting YS definitive hematopoiesis and FL colonization. 2016-11-22 /pmc/articles/PMC6940422/ /pubmed/27880904 http://dx.doi.org/10.1016/j.celrep.2016.10.080 Text en This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Lee, Lydia K.
Ghorbanian, Yasamine
Wang, Wenyuan
Wang, Yanling
Kim, Yeon Joo
Weissman, Irving L.
Inlay, Matthew A.
Mikkola, Hanna K.A.
LYVE1 Marks the Divergence of Yolk Sac Definitive Hemogenic Endothelium from the Primitive Erythroid Lineage
title LYVE1 Marks the Divergence of Yolk Sac Definitive Hemogenic Endothelium from the Primitive Erythroid Lineage
title_full LYVE1 Marks the Divergence of Yolk Sac Definitive Hemogenic Endothelium from the Primitive Erythroid Lineage
title_fullStr LYVE1 Marks the Divergence of Yolk Sac Definitive Hemogenic Endothelium from the Primitive Erythroid Lineage
title_full_unstemmed LYVE1 Marks the Divergence of Yolk Sac Definitive Hemogenic Endothelium from the Primitive Erythroid Lineage
title_short LYVE1 Marks the Divergence of Yolk Sac Definitive Hemogenic Endothelium from the Primitive Erythroid Lineage
title_sort lyve1 marks the divergence of yolk sac definitive hemogenic endothelium from the primitive erythroid lineage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940422/
https://www.ncbi.nlm.nih.gov/pubmed/27880904
http://dx.doi.org/10.1016/j.celrep.2016.10.080
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