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Increased Expression of Immature Mannose-Containing Glycoproteins and Sialic Acid in Aged Mouse Brains
Aging represents the accumulation of changes in an individual over time, encompassing physical, psychological, and social changes. Posttranslational modifications of proteins such as glycosylation, including sialylation or glycation, are proposed to be involved in this process, since they modulate a...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940728/ https://www.ncbi.nlm.nih.gov/pubmed/31817246 http://dx.doi.org/10.3390/ijms20246118 |
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author | Simon, Frieder Bork, Kaya Gnanapragassam, Vinayaga S. Baldensperger, Tim Glomb, Marcus A. Di Sanzo, Simone Ori, Alessandro Horstkorte, Rüdiger |
author_facet | Simon, Frieder Bork, Kaya Gnanapragassam, Vinayaga S. Baldensperger, Tim Glomb, Marcus A. Di Sanzo, Simone Ori, Alessandro Horstkorte, Rüdiger |
author_sort | Simon, Frieder |
collection | PubMed |
description | Aging represents the accumulation of changes in an individual over time, encompassing physical, psychological, and social changes. Posttranslational modifications of proteins such as glycosylation, including sialylation or glycation, are proposed to be involved in this process, since they modulate a variety of molecular and cellular functions. In this study, we analyzed selected posttranslational modifications and the respective proteins on which they occur in young and old mouse brains. The expression of neural cell adhesion molecule (NCAM), receptor for advanced glycation endproducts (RAGE), as well as the carbohydrate-epitopes paucimannose and high-mannose, polysialic acid, and O-GlcNAc were examined. We demonstrated that mannose-containing glycans increased on glycoproteins in aged mouse brains and identified synapsin-1 as one major carrier of paucimannose in aged brains. In addition, we found an accumulation of so-called advanced glycation endproducts, which are generated by non-enzymatic reactions and interfere with protein function. Furthermore, we analyzed the expression of sialic acid and found also an increase during aging. |
format | Online Article Text |
id | pubmed-6940728 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69407282020-01-09 Increased Expression of Immature Mannose-Containing Glycoproteins and Sialic Acid in Aged Mouse Brains Simon, Frieder Bork, Kaya Gnanapragassam, Vinayaga S. Baldensperger, Tim Glomb, Marcus A. Di Sanzo, Simone Ori, Alessandro Horstkorte, Rüdiger Int J Mol Sci Article Aging represents the accumulation of changes in an individual over time, encompassing physical, psychological, and social changes. Posttranslational modifications of proteins such as glycosylation, including sialylation or glycation, are proposed to be involved in this process, since they modulate a variety of molecular and cellular functions. In this study, we analyzed selected posttranslational modifications and the respective proteins on which they occur in young and old mouse brains. The expression of neural cell adhesion molecule (NCAM), receptor for advanced glycation endproducts (RAGE), as well as the carbohydrate-epitopes paucimannose and high-mannose, polysialic acid, and O-GlcNAc were examined. We demonstrated that mannose-containing glycans increased on glycoproteins in aged mouse brains and identified synapsin-1 as one major carrier of paucimannose in aged brains. In addition, we found an accumulation of so-called advanced glycation endproducts, which are generated by non-enzymatic reactions and interfere with protein function. Furthermore, we analyzed the expression of sialic acid and found also an increase during aging. MDPI 2019-12-04 /pmc/articles/PMC6940728/ /pubmed/31817246 http://dx.doi.org/10.3390/ijms20246118 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Simon, Frieder Bork, Kaya Gnanapragassam, Vinayaga S. Baldensperger, Tim Glomb, Marcus A. Di Sanzo, Simone Ori, Alessandro Horstkorte, Rüdiger Increased Expression of Immature Mannose-Containing Glycoproteins and Sialic Acid in Aged Mouse Brains |
title | Increased Expression of Immature Mannose-Containing Glycoproteins and Sialic Acid in Aged Mouse Brains |
title_full | Increased Expression of Immature Mannose-Containing Glycoproteins and Sialic Acid in Aged Mouse Brains |
title_fullStr | Increased Expression of Immature Mannose-Containing Glycoproteins and Sialic Acid in Aged Mouse Brains |
title_full_unstemmed | Increased Expression of Immature Mannose-Containing Glycoproteins and Sialic Acid in Aged Mouse Brains |
title_short | Increased Expression of Immature Mannose-Containing Glycoproteins and Sialic Acid in Aged Mouse Brains |
title_sort | increased expression of immature mannose-containing glycoproteins and sialic acid in aged mouse brains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940728/ https://www.ncbi.nlm.nih.gov/pubmed/31817246 http://dx.doi.org/10.3390/ijms20246118 |
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