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Simultaneous Enhancement of Thermostability and Catalytic Activity of a Metagenome-Derived β-Glucosidase Using Directed Evolution for the Biosynthesis of Butyl Glucoside

Butyl glucoside synthesis using bioenzymatic methods at high temperatures has gained increasing interest. Protein engineering using directed evolution of a metagenome-derived β-glucosidase of Bgl1D was performed to identify enzymes with improved activity and thermostability. An interesting mutant Bg...

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Detalles Bibliográficos
Autores principales:	Yin, Bangqiao, Hui, Qinyan, Kashif, Muhammad, Yu, Ran, Chen, Si, Ou, Qian, Wu, Bo, Jiang, Chengjian
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940790/ https://www.ncbi.nlm.nih.gov/pubmed/31835569 http://dx.doi.org/10.3390/ijms20246224

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