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Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s
There is a current clinical need for the development of bone void fillers and bioactive bone graft substitutes. The use of mesenchymal stem cells (MSCs) that are seeded into 3D scaffolds and induce bone generation in the event of MSCs osteogenic differentiation is highly promising. Since calcium ion...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940807/ https://www.ncbi.nlm.nih.gov/pubmed/31835689 http://dx.doi.org/10.3390/ijms20246242 |
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author | Nifant’ev, Ilya Bukharova, Tatiana Dyakonov, Alexander Goldshtein, Dmitry Galitsyna, Elena Kosarev, Maxim Shlyakhtin, Andrey Gavrilov, Dmitry Ivchenko, Pavel |
author_facet | Nifant’ev, Ilya Bukharova, Tatiana Dyakonov, Alexander Goldshtein, Dmitry Galitsyna, Elena Kosarev, Maxim Shlyakhtin, Andrey Gavrilov, Dmitry Ivchenko, Pavel |
author_sort | Nifant’ev, Ilya |
collection | PubMed |
description | There is a current clinical need for the development of bone void fillers and bioactive bone graft substitutes. The use of mesenchymal stem cells (MSCs) that are seeded into 3D scaffolds and induce bone generation in the event of MSCs osteogenic differentiation is highly promising. Since calcium ions and phosphates promote the osteogenic differentiation of MSCs, the use of the calcium complexes of phosphate-containing polymers is highly prospective in the development of osteogenic scaffolds. Calcium poly(ethylene phosphate)s (PEP-Ca) appear to be potentially suitable candidates primarily because of PEP’s biodegradability. In a series of experiments with human adipose-tissue-derived multipotent mesenchymal stem cells (ADSCs), we demonstrated that PEP-Ca are non-toxic and give rise to osteogenesis gene marker, bone morphogenetic protein 2 (BMP-2) and mineralization of the intercellular matrix. Owing to the synthetic availability of poly(ethylene phosphoric acid) block copolymers, these results hold out the possibility for the development of promising new polymer composites for orthopaedic and maxillofacial surgery. |
format | Online Article Text |
id | pubmed-6940807 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69408072020-01-09 Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s Nifant’ev, Ilya Bukharova, Tatiana Dyakonov, Alexander Goldshtein, Dmitry Galitsyna, Elena Kosarev, Maxim Shlyakhtin, Andrey Gavrilov, Dmitry Ivchenko, Pavel Int J Mol Sci Communication There is a current clinical need for the development of bone void fillers and bioactive bone graft substitutes. The use of mesenchymal stem cells (MSCs) that are seeded into 3D scaffolds and induce bone generation in the event of MSCs osteogenic differentiation is highly promising. Since calcium ions and phosphates promote the osteogenic differentiation of MSCs, the use of the calcium complexes of phosphate-containing polymers is highly prospective in the development of osteogenic scaffolds. Calcium poly(ethylene phosphate)s (PEP-Ca) appear to be potentially suitable candidates primarily because of PEP’s biodegradability. In a series of experiments with human adipose-tissue-derived multipotent mesenchymal stem cells (ADSCs), we demonstrated that PEP-Ca are non-toxic and give rise to osteogenesis gene marker, bone morphogenetic protein 2 (BMP-2) and mineralization of the intercellular matrix. Owing to the synthetic availability of poly(ethylene phosphoric acid) block copolymers, these results hold out the possibility for the development of promising new polymer composites for orthopaedic and maxillofacial surgery. MDPI 2019-12-11 /pmc/articles/PMC6940807/ /pubmed/31835689 http://dx.doi.org/10.3390/ijms20246242 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Nifant’ev, Ilya Bukharova, Tatiana Dyakonov, Alexander Goldshtein, Dmitry Galitsyna, Elena Kosarev, Maxim Shlyakhtin, Andrey Gavrilov, Dmitry Ivchenko, Pavel Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s |
title | Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s |
title_full | Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s |
title_fullStr | Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s |
title_full_unstemmed | Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s |
title_short | Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s |
title_sort | osteogenic differentiation of human adipose tissue-derived mscs by non-toxic calcium poly(ethylene phosphate)s |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940807/ https://www.ncbi.nlm.nih.gov/pubmed/31835689 http://dx.doi.org/10.3390/ijms20246242 |
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