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Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging

Our ability to observe biochemical events with high spatial and temporal resolution is essential for understanding the functioning of living systems. Intrinsically fluorescent proteins such as the green fluorescent protein (GFP) have revolutionized the way biologists study cells and organisms. The f...

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Detalles Bibliográficos
Autores principales: Péresse, Tiphaine, Gautier, Arnaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940837/
https://www.ncbi.nlm.nih.gov/pubmed/31817528
http://dx.doi.org/10.3390/ijms20246142
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author Péresse, Tiphaine
Gautier, Arnaud
author_facet Péresse, Tiphaine
Gautier, Arnaud
author_sort Péresse, Tiphaine
collection PubMed
description Our ability to observe biochemical events with high spatial and temporal resolution is essential for understanding the functioning of living systems. Intrinsically fluorescent proteins such as the green fluorescent protein (GFP) have revolutionized the way biologists study cells and organisms. The fluorescence toolbox has been recently extended with new fluorescent reporters composed of a genetically encoded tag that binds endogenously present or exogenously applied fluorogenic chromophores (so-called fluorogens) and activates their fluorescence. This review presents the toolbox of fluorogen-based reporters and biosensors available to biologists. Various applications are detailed to illustrate the possible uses and opportunities offered by this new generation of fluorescent probes and sensors for advanced bioimaging.
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spelling pubmed-69408372020-01-09 Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging Péresse, Tiphaine Gautier, Arnaud Int J Mol Sci Review Our ability to observe biochemical events with high spatial and temporal resolution is essential for understanding the functioning of living systems. Intrinsically fluorescent proteins such as the green fluorescent protein (GFP) have revolutionized the way biologists study cells and organisms. The fluorescence toolbox has been recently extended with new fluorescent reporters composed of a genetically encoded tag that binds endogenously present or exogenously applied fluorogenic chromophores (so-called fluorogens) and activates their fluorescence. This review presents the toolbox of fluorogen-based reporters and biosensors available to biologists. Various applications are detailed to illustrate the possible uses and opportunities offered by this new generation of fluorescent probes and sensors for advanced bioimaging. MDPI 2019-12-05 /pmc/articles/PMC6940837/ /pubmed/31817528 http://dx.doi.org/10.3390/ijms20246142 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Péresse, Tiphaine
Gautier, Arnaud
Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging
title Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging
title_full Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging
title_fullStr Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging
title_full_unstemmed Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging
title_short Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging
title_sort next-generation fluorogen-based reporters and biosensors for advanced bioimaging
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940837/
https://www.ncbi.nlm.nih.gov/pubmed/31817528
http://dx.doi.org/10.3390/ijms20246142
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