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Oxidative Damage of DNA as Early Marker of Alzheimer’s Disease
Alzheimer’s Disease (AD) is the most common cause of dementia, and its characteristic histopathological hallmarks are neurofibrillary tangles and senile plaques. Among involved mechanisms, oxidative stress plays an important role in damaging cell components (e.g., proteins, nucleic acids). In this s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940966/ https://www.ncbi.nlm.nih.gov/pubmed/31817451 http://dx.doi.org/10.3390/ijms20246136 |
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author | Peña-Bautista, Carmen Tirle, Tania López-Nogueroles, Marina Vento, Máximo Baquero, Miguel Cháfer-Pericás, Consuelo |
author_facet | Peña-Bautista, Carmen Tirle, Tania López-Nogueroles, Marina Vento, Máximo Baquero, Miguel Cháfer-Pericás, Consuelo |
author_sort | Peña-Bautista, Carmen |
collection | PubMed |
description | Alzheimer’s Disease (AD) is the most common cause of dementia, and its characteristic histopathological hallmarks are neurofibrillary tangles and senile plaques. Among involved mechanisms, oxidative stress plays an important role in damaging cell components (e.g., proteins, nucleic acids). In this study, different oxidized products of proteins and DNA were determined in the urine samples from mild cognitive impairment due to AD patients (n = 53) and healthy controls (n = 27) by means of ultra-performance liquid chromatography-tandem mass spectrometry analysis. A multivariate model developed by partial least squares generated a diagnostic model for AD with an AUC-ROC (area under the curve-receiver operating characteristic) of 0.843. From the studied analytes, 8-OHdG (8-hydroxy-2’-deoxyguanosine) and the ratio 8-OHdG/2dG (2’-deoxyguanosine) were able to distinguish between AD and healthy participants, showing statistically significant differences between groups, postulating DNA oxidation as a molecular pathway involved in early AD. |
format | Online Article Text |
id | pubmed-6940966 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69409662020-01-09 Oxidative Damage of DNA as Early Marker of Alzheimer’s Disease Peña-Bautista, Carmen Tirle, Tania López-Nogueroles, Marina Vento, Máximo Baquero, Miguel Cháfer-Pericás, Consuelo Int J Mol Sci Article Alzheimer’s Disease (AD) is the most common cause of dementia, and its characteristic histopathological hallmarks are neurofibrillary tangles and senile plaques. Among involved mechanisms, oxidative stress plays an important role in damaging cell components (e.g., proteins, nucleic acids). In this study, different oxidized products of proteins and DNA were determined in the urine samples from mild cognitive impairment due to AD patients (n = 53) and healthy controls (n = 27) by means of ultra-performance liquid chromatography-tandem mass spectrometry analysis. A multivariate model developed by partial least squares generated a diagnostic model for AD with an AUC-ROC (area under the curve-receiver operating characteristic) of 0.843. From the studied analytes, 8-OHdG (8-hydroxy-2’-deoxyguanosine) and the ratio 8-OHdG/2dG (2’-deoxyguanosine) were able to distinguish between AD and healthy participants, showing statistically significant differences between groups, postulating DNA oxidation as a molecular pathway involved in early AD. MDPI 2019-12-05 /pmc/articles/PMC6940966/ /pubmed/31817451 http://dx.doi.org/10.3390/ijms20246136 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Peña-Bautista, Carmen Tirle, Tania López-Nogueroles, Marina Vento, Máximo Baquero, Miguel Cháfer-Pericás, Consuelo Oxidative Damage of DNA as Early Marker of Alzheimer’s Disease |
title | Oxidative Damage of DNA as Early Marker of Alzheimer’s Disease |
title_full | Oxidative Damage of DNA as Early Marker of Alzheimer’s Disease |
title_fullStr | Oxidative Damage of DNA as Early Marker of Alzheimer’s Disease |
title_full_unstemmed | Oxidative Damage of DNA as Early Marker of Alzheimer’s Disease |
title_short | Oxidative Damage of DNA as Early Marker of Alzheimer’s Disease |
title_sort | oxidative damage of dna as early marker of alzheimer’s disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6940966/ https://www.ncbi.nlm.nih.gov/pubmed/31817451 http://dx.doi.org/10.3390/ijms20246136 |
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