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“Salicylic Acid Mutant Collection” as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment

The phytohormone salicylic acid (SA) has a crucial role in plant physiology. Its role is best described in the context of plant response to pathogen attack. During infection, SA is rapidly accumulated throughout the green tissues and is important for both local and systemic defences. However, some g...

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Autores principales: Pluhařová, Kamila, Leontovyčová, Hana, Stoudková, Věra, Pospíchalová, Romana, Maršík, Petr, Klouček, Pavel, Starodubtseva, Anastasiia, Iakovenko, Oksana, Krčková, Zuzana, Valentová, Olga, Burketová, Lenka, Janda, Martin, Kalachova, Tetiana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941003/
https://www.ncbi.nlm.nih.gov/pubmed/31861218
http://dx.doi.org/10.3390/ijms20246365
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author Pluhařová, Kamila
Leontovyčová, Hana
Stoudková, Věra
Pospíchalová, Romana
Maršík, Petr
Klouček, Pavel
Starodubtseva, Anastasiia
Iakovenko, Oksana
Krčková, Zuzana
Valentová, Olga
Burketová, Lenka
Janda, Martin
Kalachova, Tetiana
author_facet Pluhařová, Kamila
Leontovyčová, Hana
Stoudková, Věra
Pospíchalová, Romana
Maršík, Petr
Klouček, Pavel
Starodubtseva, Anastasiia
Iakovenko, Oksana
Krčková, Zuzana
Valentová, Olga
Burketová, Lenka
Janda, Martin
Kalachova, Tetiana
author_sort Pluhařová, Kamila
collection PubMed
description The phytohormone salicylic acid (SA) has a crucial role in plant physiology. Its role is best described in the context of plant response to pathogen attack. During infection, SA is rapidly accumulated throughout the green tissues and is important for both local and systemic defences. However, some genetic/metabolic variations can also result in SA overaccumulation in plants, even in basal conditions. To date, more than forty Arabidopsis thaliana mutants have been described as having enhanced endogenous SA levels or constitutively activated SA signalling pathways. In this study, we established a collection of mutants containing different SA levels due to diverse genetic modifications and distinct gene functions. We chose prototypic SA-overaccumulators (SA-OAs), such as bon1-1, but also “non-typical” ones such as exo70b1-1; the selection of OA is accompanied by their crosses with SA-deficient lines. Here, we extensively studied the plant development and SA level/signalling under various growth conditions in soil and in vitro, and showed a strong negative correlation between rosette size, SA content and PR1/ICS1 transcript signature. SA-OAs (namely cpr5, acd6, bon1-1, fah1/fah2 and pi4kβ1β2) had bigger rosettes under high light conditions, whereas WT plants did not. Our data provide new insights clarifying a link between SA and plant behaviour under environmental stresses. The presented SA mutant collection is thus a suitable tool to shed light on the mechanisms underlying trade-offs between growth and defence in plants.
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spelling pubmed-69410032020-01-09 “Salicylic Acid Mutant Collection” as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment Pluhařová, Kamila Leontovyčová, Hana Stoudková, Věra Pospíchalová, Romana Maršík, Petr Klouček, Pavel Starodubtseva, Anastasiia Iakovenko, Oksana Krčková, Zuzana Valentová, Olga Burketová, Lenka Janda, Martin Kalachova, Tetiana Int J Mol Sci Article The phytohormone salicylic acid (SA) has a crucial role in plant physiology. Its role is best described in the context of plant response to pathogen attack. During infection, SA is rapidly accumulated throughout the green tissues and is important for both local and systemic defences. However, some genetic/metabolic variations can also result in SA overaccumulation in plants, even in basal conditions. To date, more than forty Arabidopsis thaliana mutants have been described as having enhanced endogenous SA levels or constitutively activated SA signalling pathways. In this study, we established a collection of mutants containing different SA levels due to diverse genetic modifications and distinct gene functions. We chose prototypic SA-overaccumulators (SA-OAs), such as bon1-1, but also “non-typical” ones such as exo70b1-1; the selection of OA is accompanied by their crosses with SA-deficient lines. Here, we extensively studied the plant development and SA level/signalling under various growth conditions in soil and in vitro, and showed a strong negative correlation between rosette size, SA content and PR1/ICS1 transcript signature. SA-OAs (namely cpr5, acd6, bon1-1, fah1/fah2 and pi4kβ1β2) had bigger rosettes under high light conditions, whereas WT plants did not. Our data provide new insights clarifying a link between SA and plant behaviour under environmental stresses. The presented SA mutant collection is thus a suitable tool to shed light on the mechanisms underlying trade-offs between growth and defence in plants. MDPI 2019-12-17 /pmc/articles/PMC6941003/ /pubmed/31861218 http://dx.doi.org/10.3390/ijms20246365 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pluhařová, Kamila
Leontovyčová, Hana
Stoudková, Věra
Pospíchalová, Romana
Maršík, Petr
Klouček, Pavel
Starodubtseva, Anastasiia
Iakovenko, Oksana
Krčková, Zuzana
Valentová, Olga
Burketová, Lenka
Janda, Martin
Kalachova, Tetiana
“Salicylic Acid Mutant Collection” as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment
title “Salicylic Acid Mutant Collection” as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment
title_full “Salicylic Acid Mutant Collection” as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment
title_fullStr “Salicylic Acid Mutant Collection” as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment
title_full_unstemmed “Salicylic Acid Mutant Collection” as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment
title_short “Salicylic Acid Mutant Collection” as a Tool to Explore the Role of Salicylic Acid in Regulation of Plant Growth under a Changing Environment
title_sort “salicylic acid mutant collection” as a tool to explore the role of salicylic acid in regulation of plant growth under a changing environment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941003/
https://www.ncbi.nlm.nih.gov/pubmed/31861218
http://dx.doi.org/10.3390/ijms20246365
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