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Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles
The cellular vesicle is a fluid-filled structure separated from the surrounding environment by a biological membrane. Here, we isolated nanovesicles (NVs) from the juice of clementines using a discontinuous density gradient ultracentrifugation method. To gain information about the protein content of...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941005/ https://www.ncbi.nlm.nih.gov/pubmed/31835328 http://dx.doi.org/10.3390/ijms20246205 |
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author | Stanly, Christopher Moubarak, Maneea Fiume, Immacolata Turiák, Lilla Pocsfalvi, Gabriella |
author_facet | Stanly, Christopher Moubarak, Maneea Fiume, Immacolata Turiák, Lilla Pocsfalvi, Gabriella |
author_sort | Stanly, Christopher |
collection | PubMed |
description | The cellular vesicle is a fluid-filled structure separated from the surrounding environment by a biological membrane. Here, we isolated nanovesicles (NVs) from the juice of clementines using a discontinuous density gradient ultracentrifugation method. To gain information about the protein content of vesicles, mass spectrometry-based organelle proteomics and bioinformatics were applied to the exosome-like vesicle fraction isolated in the 1 mol/L sucrose/D(2)O cushion. Analysis of 1018 identified proteins revealed a highly complex mixture of different intra, extracellular and artificially-formed vesicle populations. In particular, clathrin-coated vesicles were significantly expressed in this sample. Membrane transporters are significantly represented in clementines nanovesicles. We have found 162 proteins associated with the transport Gene Ontology term (GO: 0006810) which includes; 71 transmembrane transport related, 53 vesicle mediated and 50 intracellular transporters. Platellin-3 like carrier protein containing a Sec14 domain is known to have a role in plant-virus interaction and that is one of the most abundant proteins in our dataset. The presence of transmembrane transporters like ATPases, aquaporins, ATP Binding Cassette (ABC) transporters and tetraspanins, regulators of protein trafficking suggests that nanovesicles of clementines can actively interact with their environment in a controlled way. |
format | Online Article Text |
id | pubmed-6941005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69410052020-01-09 Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles Stanly, Christopher Moubarak, Maneea Fiume, Immacolata Turiák, Lilla Pocsfalvi, Gabriella Int J Mol Sci Article The cellular vesicle is a fluid-filled structure separated from the surrounding environment by a biological membrane. Here, we isolated nanovesicles (NVs) from the juice of clementines using a discontinuous density gradient ultracentrifugation method. To gain information about the protein content of vesicles, mass spectrometry-based organelle proteomics and bioinformatics were applied to the exosome-like vesicle fraction isolated in the 1 mol/L sucrose/D(2)O cushion. Analysis of 1018 identified proteins revealed a highly complex mixture of different intra, extracellular and artificially-formed vesicle populations. In particular, clathrin-coated vesicles were significantly expressed in this sample. Membrane transporters are significantly represented in clementines nanovesicles. We have found 162 proteins associated with the transport Gene Ontology term (GO: 0006810) which includes; 71 transmembrane transport related, 53 vesicle mediated and 50 intracellular transporters. Platellin-3 like carrier protein containing a Sec14 domain is known to have a role in plant-virus interaction and that is one of the most abundant proteins in our dataset. The presence of transmembrane transporters like ATPases, aquaporins, ATP Binding Cassette (ABC) transporters and tetraspanins, regulators of protein trafficking suggests that nanovesicles of clementines can actively interact with their environment in a controlled way. MDPI 2019-12-09 /pmc/articles/PMC6941005/ /pubmed/31835328 http://dx.doi.org/10.3390/ijms20246205 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Stanly, Christopher Moubarak, Maneea Fiume, Immacolata Turiák, Lilla Pocsfalvi, Gabriella Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles |
title | Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles |
title_full | Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles |
title_fullStr | Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles |
title_full_unstemmed | Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles |
title_short | Membrane Transporters in Citrus clementina Fruit Juice-Derived Nanovesicles |
title_sort | membrane transporters in citrus clementina fruit juice-derived nanovesicles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6941005/ https://www.ncbi.nlm.nih.gov/pubmed/31835328 http://dx.doi.org/10.3390/ijms20246205 |
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